Tea seedling mycorrhization cutting breeding new method
A new method, mycorrhizalization technology, applied in botany equipment and methods, vegetative propagation, horticulture, etc., can solve problems such as low survival rate, poor quality of tea seedlings, and long breeding cycle
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[0026] The new method for cutting and breeding tea seedlings of the invention is to promote the cutting and breeding of tea seedlings by inoculating arbuscular mycorrhizal fungi. Include the following steps:
[0027] a. Separation of strains
[0028] The arbuscular mycorrhizal fungi were derived from the dominant species in the rhizosphere of tea trees. The tea tree rhizosphere soil was taken, and the dominant monospores of arbuscular mycorrhizal fungi were isolated by wet sieving-sucrose centrifugation.
[0029] b. Propagation of bacteria
[0030] Take yellow sand soil (need to pass through a 2mm sieve) and river sand in a ratio of 1:1, sterilize it by irradiation (12.5kGy60Co), and then plant white clover for propagation. The clover seeds are washed with 10% NaOH for 3-5 minutes, washed with distilled water several times, soaked in 75% alcohol for 20 minutes, washed with distilled water, dried and sown with water and air.
[0031] c. Seedbed preparation
[0032] The tea...
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