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A.C.C. extract having anti-inflammatory and antibacterial effects and composition containing the same as active ingredient

A technology of extracts and weight percentages, applied in the field of extracts and antibacterial and anti-inflammatory compositions, which can solve problems such as aggravated inflammation

Inactive Publication Date: 2019-04-19
禹勇奎
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is well known that NO in the body plays an important role in antibacterial and tumor resection, but the NO formed by iNOS causes vascular permeability increase, edema and other reactions, promotes the synthesis of inflammatory mediators, and aggravates inflammation

Method used

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  • A.C.C. extract having anti-inflammatory and antibacterial effects and composition containing the same as active ingredient
  • A.C.C. extract having anti-inflammatory and antibacterial effects and composition containing the same as active ingredient
  • A.C.C. extract having anti-inflammatory and antibacterial effects and composition containing the same as active ingredient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The selection of embodiment 1. material and the preparation of A.C.C. extract

[0030] Based on Materia Medica, a document survey was carried out, and a total of 14 kinds of Chinese medicinal materials were selected. Chinese herbal medicines were purchased from CK Co., Ltd. in Yangnyeong-si, Seoul, which were packaged and sold as crude drug preparations. Dulbecco’s modified eagle’s medium (DMEM) and fetal bovine serum (FBS) were purchased from Hyclone (Logan, UT, USA). Penicillin (100U / mL) and streptomycin (100ug / ml) were purchased from Gibco (Life Technology Inc., Gaithersburg, MD, USA, Life technology 1nc., Gaithersburg, MD, USA) . LPS, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (3-(4,5-Dimethylthiazol-2-y1)-2,5- diphenyl tetrazolium bromide, MTT), sodium nitrite (SodiumNitrite), dimethyl sulfoxide (Dimethyl sulfoxide) (DMSO) were purchased from Sigma Chemical Co. (St.Louis, MO, USA). The Nitrite oxide (NO) detection kit (detection Kit) was purch...

experiment example 1

[0035] Experimental example 1: Cytotoxicity assay based on MTT assay

[0036] Culture of Raw 264.7 cells

[0037] Raw 264.7 cells were purchased from Korea Cell Line Bank. Using DMEM medium containing Penicillin / streptomycin 100 unit / ml and 10% FBS, cultured in 37°C, 5% CO2 incubator, and subcultured once every 3 days.

[0038] Raw 264.7 cell viability assay, the 96-well plate (well plate) was adjusted to 2 × 10 4 After the concentration of cells / well, culture for 24 hours, perform stabilization. After 24 hours, the medium of the well was completely removed. The A.C.C. extract was diluted in the culture medium so that the final concentration of the A.C.C. extract was 1, 5, and 10 μg / ml, and treated with the cell line, and cultured for 3 hours. After 3 hours, the wells except the control group were treated with LPS 1 μg / ml. After 24 hours, the medium was removed, and 100 μl of MTT solution (0.2 μg / ml in phenol red free DMEM, 0.2 μg / ml in phenol red free DMEM) was added to ...

experiment example 2

[0039] Experimental Example 2: Measurement of Nitric Oxide Production

[0040] In 6well plate, with 1×10 5 For the concentration of cells / well, add Raw 264.7 cells. After storage at 37°C in a 5% CO2 incubator for 24 hours, Raw 264.7 cells were treated with 1, 5, and 10 μg / ml A.C.C. extract, 3 hours later, treated with LPS 1 μg / ml, and cultured for 24 hours. Nitric oxide was analyzed using a nitrite oxide (NO) detection kit (iNtron) based on the Griess reaction. Griess Reagent was added to the cell culture supernatant and mixed, reacted at room temperature for 10 minutes, and measured at 520 nm absorbance using a microplate reader. Standard curves based on different concentrations of sodium nitrite (Sodium nitrite) are suitable for use in the formula to determine the NO concentration in the culture solution.

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Abstract

The present invention provides an A.C.C. extract and a composition containing the same as active ingredient. The A.C.C. extract is prepared by combining Chinese herbal medicines of Phellodendron bark,Scutellaria baicalensis, Paeonia lactiflora Pall, Dictamnus dasycarpus Turcz, Anemarrhena asphodeloides, Alumen, Dryobalanops aromatica Gaertner, Mentha arvensis var. Piperascens, Inula helenium, Syringa velutina var. Kamibayashii, Corydalis incisa, Eclipta prostrata, Lonicera japonica and Glycyrrhiza uralensis. The A.C.C. extract of the present invention exhibits reduction of tumor necrosis factor-alpha (TNF-alpha), interleukin-1Beta (IL-1Beta) and interleukin-6 (IL-6), is a substance that regulates the expression of iNOS to inhibit the anti-inflammatory effect of NO production, is a substance that relieves symptoms such as diaper rash and lice by clinical anti-inflammatory and anti-bacterial effects, and can be used as an effective inflammatory therapeutic agent.

Description

technical field [0001] The present invention relates to an extract having anti-inflammatory and anti-bacterial effects and an anti-bacterial and anti-inflammatory composition. Background technique [0002] The inflammatory response caused in the body is one of the important body defense mechanisms for recovering damaged parts when receiving physical and chemical stimuli such as scars or bacterial infections. When stimulated, vasoactive substances are locally released, thereby increasing vascular permeability, Induce inflammation. In addition, the inflammatory reaction is a recovery system formed in the living body according to external stimuli, and it appears clinically with seizures, fever, swelling, pain, and the like. Inflammation-related cells include macrophages, monocytes, lymphocytes, mast cells, fibroblasts, platelets, etc. Macrophages are called the main cells that cause inflammation. Or activated by cytokines secreted by immune cells to generate nitric oxide (NO)...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8964A61P29/00A61P31/04A61K33/06
CPCA61K36/8964A61P29/00A61P31/04A61K33/06A61K36/185A61K36/28A61K36/355A61K36/484A61K36/534A61K36/539A61K36/63A61K36/65A61K36/66A61K36/71A61K36/75A61K36/756A61K2300/00A61K36/505
Inventor 禹勇奎
Owner 禹勇奎
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