Method for detecting behcet diseases

Abstract

The invention relates to a method for detecting behcet diseases. The method comprises the steps that data of 15 behcet disease HLA allelic gene polymorphism types is collected, a behcet disease HLA allelic gene polymorphism database is established, according to the 15 behcet disease HLA allelic gene polymorphism types in the behcet disease HLA allelic gene polymorphism database, 15 behcet diseaseHLA typing specific primers are designed, after a to-be-detected DNA sample is extracted, the behcet disease HLA typing specific primers and the to-be-detected DNA sample are added into a sequencing chip, amplification and sequencing are performed on the to-be-detected DNA sample, the HLA type of the to-be-detected DNA sample in a sequencing result is compared with the 15 behcet disease HLA allelic gene polymorphism types in the database, a comparison result is judged by a physician, and the auxiliary diagnosis of the behcet diseases is achieved.

Description

technical field [0001] The invention relates to the field of computer technology, in particular to a detection method for Behcet's disease. Background technique [0002] Uveitis is a common ophthalmic disease, which is often accompanied by a series of ocular and systemic complications, and is a global blinding eye disease. There are various types of uveitis, and the clinical manifestations vary greatly among individuals. Therefore, doctors have great challenges in diagnosis and treatment. [0003] Human leukocyte antigen (human lymphocyte antigen, HLA) is a highly polymorphic alloantigen, its chemical essence is a type of glycoprotein, which consists of an α heavy chain (glycosylated) and a β light chain non-covalently bound. Its distribution and function are divided into class I antigens and class II antigens. HLA polymorphisms depend on the β light chain. It is conservatively estimated that there are at least 1300 different haplotypes, corresponding to about 17×107 geno...

AI Technical Summary

Problems solved by technology

Although Leiden University has established a database (LOVD) of human gene mutations and polymorphisms associated with various diseases, it does not cover the relevant data of uveitis

The possible reasons are that, as mentioned above, there are various types of uveitis, large individual differences in the clinical manifestations of the population, and the lack of a unified collation and management model in the global collection of HLA polymorphism data

[0006] Moreover, due to the diversity of uveitis types and the large individual differences in clinical manifestations, there will still be a high rate of misdiagnosis based on the existing clinical manifestations, especially for young doctors. It is a big challenge, so in addition to clinical manifestations and routine testing methods, the diagnosis of uveitis still needs more auxiliary examination methods, such as the genetic diagnosis mentioned above

[0007] At present, clinicians mainly diagnose Behcet's disease through routine methods such as patients' clinical manifestations and ultrasonography. The examination methods are very limited, and it is difficult to distinguish it from other diseases.

Due to the large individual differences in clinical manifestations, there will still be a high rate of misdiagnosis based on existing clinical manifestations. Especially for young doctors, the diagnosis of Behcet’s disease is very challenging. Therefore, in addition to clinical manifestations and Conventional inspection methods require more auxiliary inspection methods to judge Behcet's disease

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