Perfume prepared by Debaryomyces hansenii yeast fermentation
A technology for fermentation of flavors and fragrances and yeast, applied in the field of flavors and fragrances, can solve the problems of fermentation and production of natural flavors and fragrances, obstacles, etc., and achieve the effects of low cost, simple process and high safety
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Embodiment 1
[0051] Embodiment 1: Debaria hansenii+Sophora japonica
[0052] Specific steps are as follows:
[0053] (1) Take a loop of the yeast strain stored at 4°C and transfer it to the YPD seed medium (sterilized at 115°C for 20 minutes), at 30°C, with a rotation speed of 200r min -1 Cultivate for 2 days under certain conditions to obtain the activation solution;
[0054] (2) Take 80mL of YPD seed medium and place it in a 250mL Erlenmeyer flask (sterilized at 115°C for 20min), take 200μL of the activation solution obtained in (1) and inoculate it into the YPD seed medium, at a temperature of 30°C and a rotation speed of 200r·min -1 Cultivate for 2 days under the condition of 9 CFU / mL;
[0055] (3) Take 100g of fermentation medium (sterilized at 105°C for 20min) and place it in a 250mL shake flask, inoculate the seed liquid into the fermentation medium according to the parameters in Table 2 for fermentation, and obtain fermentation product A 0 、A 1 、A 2 、A 3 ; where, A 0 group ...
Embodiment 2
[0075] Embodiment 2: Debaryoyces hansenii+Paeoniae Alba
[0076] Specific steps are as follows:
[0077] Sophora pagoda in embodiment 1 is replaced into Radix Paeoniae Alba, repeats A in embodiment 1 1 Group experiment, get fermented product A 4 . For the fermented product A obtained 4 Smell the fragrance, and the results are shown in Table 6.
Embodiment 3
[0078] Embodiment 3: Debaria hansenii + lavender
[0079] Specific steps are as follows:
[0080] Sophora pagoda in embodiment 1 is replaced with lavender, repeats A in embodiment 1 1 Group experiment, get fermented product A 5 . For the fermented product A obtained 5 Smell the fragrance, and the results are shown in Table 6.
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