Porcine frozen semen unfreezing liquid as well as preparation method and unfreezing method thereof

A technology of thawing liquid and frozen semen, which is applied in the field of pig frozen semen, can solve the problems of high price, unfavorable promotion of frozen semen, unsatisfactory effect of thawing frozen semen, etc.

Inactive Publication Date: 2019-05-14
北京田园奥瑞生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no domestic special thawing solution or dilution powder for frozen semen in the market at present, and the effect of thawing frozen semen with fresh semen diluent is not ideal; while the thawing effect of imported products is good, but the price is expensive, which is not conducive to the promotion of frozen semen

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The pig frozen semen thawing solution provided in this example contains, per 1000ml: 24.54g of anhydrous glucose, 7.02g of anhydrous sodium citrate, 6g of Tris, 2.65g of anhydrous citric acid, 1.42g of potassium dihydrogen phosphate and cysteine 1mM.

[0029] The preparation method of this pig frozen semen thawing liquid is as follows:

[0030] Weigh: Glucose monohydrate 27g, sodium citrate dihydrate 8g, Tris 6g, citric acid monohydrate 2.9g, potassium dihydrogen phosphate 1.42g and cysteine ​​hydrochloride 0.175g, dissolve in 600ml double distilled water or super Pure water, dilute to 1000ml.

[0031] Osmotic pressure: 330mOsm; pH: 6.8-7.2.

experiment example

[0033] In addition, an imported thawing solution Androhep CryoGuard Thaw Extender (purchased from Mofa Company, USA) was prepared as a control thawing solution according to the instructions.

[0034] Put 1.5 ml of the two thawing solutions (the thawing solution of Example 1 and the control thawing solution) in a 2 ml centrifuge tube, and place in a 30° C. water bath to preheat. Adjust the temperature of another water bath to 50°C, take a 0.5ml thin tube (the specification is a thin tube of 0.5ml) and thaw the frozen pig semen in a water bath at 50°C for 15-16s, wipe the thin tube clean, and divide the thin tube semen into two equal parts , were added to the two thawing solutions, mixed, incubated for 15-20min.

[0035] Two semen samples were analyzed with the CASA system and PM (proportion of forward motile sperm) and TM (proportion of total motile sperm) were recorded. Repeat 6 times. The results are shown in Table 1.

[0036] Table 1

[0037]

[0038] As can be...

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PUM

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Abstract

The invention discloses a porcine frozen semen unfreezing liquid as well as a preparation method and an unfreezing method thereof, and relates to the technical field of porcine frozen semens. The porcine frozen semen comprises the following components: glucose, sodium citrate, Tris, citric acid, monopotassium phosphate and cysteine. The porcine frozen semen unfreezing liquid has a good unfreezingeffect, so that the porcine semens can keep reactively good activity.

Description

technical field [0001] The invention relates to the technical field of frozen pig semen, in particular to a thawing liquid for frozen pig semen, a preparation method and a thawing method thereof. Background technique [0002] As a very mature and practical technology, artificial insemination with normal temperature semen of pigs is widely used in pig production. However, due to the fact that normal temperature semen is not suitable for long-term storage and long-distance transportation, there are certain limitations in its use. [0003] Pig frozen semen technology plays an important role in improving the utilization efficiency of breeding pigs and preserving germplasm resources. However, there is no domestic special thawing solution or dilution powder for pig frozen semen in the market at present, and the effect of thawing frozen semen with fresh semen diluent is unsatisfactory; while imported products have good thawing effect, but they are expensive, which is not conducive ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 张士海孟克杰朱宽峰邓晓彬
Owner 北京田园奥瑞生物科技有限公司
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