The invention belongs to the technical field of quick-frozen food processing, and particularly relates to a quick-frozen conditioning grilled fish stick and a preparation method thereof. The quick-frozen conditioning grilled fish stick provided by the invention comprises stuffing and wrappers; and the preparation method comprises the following steps: carrying out microwave unfreezing on pork and chicken breast and cleaning, and cutting and mincing the pork; adding starch, ice water, phosphate, white sugar, table salt, aginomoto, black pepper, plant haematochrome and edible flavor and fragrance, and chopping to prepare the stuffing; carrying out vacuum unfreezing on fresh water fish, chicken and pork and cleaning, and cutting and mincing the pork; adding starch, emulsion paste, ice water, phosphate, salt, white sugar, aginomoto, plant haematochrome and edible flavor and fragrance, and chopping to prepare the stuffing and the wrappers; and molding the stuffing and the wrappers, stewing, quickly freezing, and packing to prepare the quick-frozen conditioning grilled fish stick.