Preparation method of phosphatidyl glycerol inhibitor

Abstract

The invention relates to a preparation method of a phosphatidyl glycerol inhibitor, belonging to the technical field of separation and purification of natural medicines. The method comprises the following steps: firstly, soaking macroporous cation exchange resin in an acid solution, draining the acid solution, and rinsing with water; then, soaking in an alkali solution, and rinsing with water; then, soaking in the acid solution, rinsing with deionized water, and then using lower alcohol for replacing; adding glycerol phosphate into the lower alcohol, adding alkali for carrying out a reaction,and then performing suction filtration; washing with the low alcohol, adjusting pH to be weakly alkaline, concentrating under reduced pressure and cooling, then transferring into a separating funnel,standing, and separating out a lower layer lower alcohol phase; packing the glycerol phosphate and phenolic resin in a column, carrying out on-column absorption on alcoholysis mixed liquid, washing the resin with the lower alcohol for removing impurities, and eluting the resin with pure water; carrying out decolorization by using a decolorizing agent, and concentrating under reduced pressure to obtain the finished product. The preparation method has the advantages that the technological steps are simple and short, and the requirements of industrial production are met; the resources are saved,and the cost is lowered; the total yield of the finished product reaches 65.47-86.08%, the external standard content reaches 99% or above, and the optical purity reaches 99%.

Description

technical field [0001] The invention belongs to the technical field of separation and purification of natural medicines, and in particular relates to a preparation method of a phosphatidylglycerol inhibitor. Background technique [0002] Phosphatidylglycerol is widely distributed in the biological world, and in microorganisms, it is sometimes the main component of phospholipids. Like cardiolipin, phosphatidylinositol, is an acidic phospholipid. In growing E. coli, it is metabolized at a higher rate than other phospholipids. It is biosynthesized from CDP glyceride and phosphoglycerol to phosphatidylglycerol, which is then dephosphorylated to form phosphatidylglycerol. The natural phosphatidylglycerol is diacyl-L-3-phosphoglycerol-D-3-glycerol. Through the action of phospholipase A, lysophosphatidylglycerol is generated. In microorganisms, there are also O-amino acid esters of phosphatidylglycerol, and the known amino acids are alanine, lysine, glutamic acid, aspartic acid...

AI Technical Summary

Problems solved by technology

It can be seen that the efficiency of this method is low, the process is more complicated, and the final yield is not ideal

Subsequently, Brockerhoff used the recrystallization method to prepare a new type of pure phosphatidylglycerol, but it was necessary to remove the catalyst and recrystallize and purify the product. The whole process was complicated and costly, and it was not suitable for commercial scale production.