Test strip for detecting wide spectrum type salmonella in food and preparation and use method thereof
A Salmonella, broad-spectrum technology, applied in the field of test strips for detecting broad-spectrum Salmonella in food, can solve the problems of complex operation, time-consuming detection, and high equipment requirements, and achieve the effects of simple process, convenient operation and disease prevention.
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[0038] In one embodiment, the preparation method includes the following steps:
[0039] a. utilize chloroauric acid solution and sodium citrate solution to prepare colloidal gold, obtain colloidal gold solution;
[0040] b. Under alkaline conditions, adding Salmonella polymonoclonal antibody DSP1 to the colloidal gold solution for labeling treatment, after sealing, centrifugation, and resuspension, the colloidal gold-labeled Salmonella polymonoclonal antibody DSP1 was obtained;
[0041] c. Soak the glass cellulose membrane in the conjugation pad treatment solution to prepare a gold standard pad; use colloidal gold-labeled Salmonella polymonoclonal antibody DSP1 to spray gold on the gold standard pad, and dry to obtain a conjugation pad ;
[0042] d. Take the Salmonella monoclonal antibody SP1 and the goat anti-rabbit IgG antibody, and draw them to the positions of the detection line and the quality control line of the nitrocellulose membrane with a film-drawing instrument;
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Embodiment 1
[0066] A test strip for detecting broad-spectrum salmonella in food
[0067] Such as figure 1 As shown, test strips for detecting broad-spectrum Salmonella in food,
[0068] Including a PVC bottom plate and a nitrocellulose membrane 4 combined in the middle of at least one surface of the PVC bottom plate 1, and an absorbent filter paper 5 arranged at one end of at least one surface of the PVC bottom plate 1 and adjacent to the nitrocellulose membrane 4. The other end of at least one surface of the PVC bottom plate 1 and the bonding pad 3 adjacent to the nitrocellulose membrane 4, and the sample adjacent to the bonding pad 3 and arranged at the end away from the nitrocellulose membrane 4 Pad 2; wherein, the nitrocellulose membrane 4 is provided with a detection line 41 and a quality control line 42 near the binding pad 3 and the water-absorbing filter paper 5 respectively; the detection line 41 is coated with Salmonella monoclonal antibody SP1; the The quality control line 42...
Embodiment 2
[0070] A method for preparing a test strip for detecting broad-spectrum salmonella in food, comprising the steps of:
[0071] a. Measure 120mL of purified water into a conical flask, heat to 340°C, stir at 300rpm until boiling, add 2mL of 1% chloroauric acid solution, heat for 2min, and quickly add 2mL of 2% lemon Sodium acid solution, continue heating for 5min, the color of the liquid is stable to wine red, cool at room temperature, and obtain a colloidal gold solution with a particle size of 35-45nm;
[0072] b. Vector take 10mL colloidal gold solution, add 0.2M K 2 CO 3 solution, adjust the pH to 8.2±0.05, place it on a shaker at a speed of 80rpm for 5min, add 150μg of Salmonella antibody, carry out labeling treatment for 30min, add 100M 10% BSA solution, and carry out blocking treatment for 30min, at 15000rpm, 4°C Centrifuge for 15 minutes, discard the supernatant, and resuspend the precipitate with 600 μL gold-labeled resuspension solution to obtain colloidal gold-label...
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