A method for preparing r-4-chloro-3-hydroxybutyric acid ethyl ester by coupling extraction with enzyme membrane reactor

Abstract

The invention relates to a method for preparing r-4-chloro-3-hydroxybutyrate ethyl by coupling extraction with an enzyme-membrane reactor, comprising an enzyme-membrane reactor, an organic solvent barrel and a substrate barrel, the enzyme-membrane reactor The interior of the enzyme membrane reactor is distributed with a filter membrane, and the upper end of the enzyme membrane reactor is connected to a feed liquid outlet, one side of the enzyme membrane reactor is provided with an organic solvent outlet, and the lower end of the enzyme membrane reactor is connected to a feed liquid inlet, An organic solution inlet is installed on the other side of the enzyme membrane reactor. The method is simple in process, reduces the emulsification phenomenon in the reaction and extraction process, improves the yield, reduces the loss of the organic solvent, does not need to extract multiple times, is simple and convenient to operate, is conducive to large-scale industrial production, and can continuously collect products. The inhibition of the product to the enzyme or the toxicity to the cell is removed, and the molar conversion rate is improved, which is more conducive to industrial production.

Description

technical field

[0001] The invention relates to the technical field of enzyme engineering, in particular to a method for preparing ethyl r-4-chloro-3-hydroxybutyrate by coupled extraction with an enzyme-membrane reactor. Background technique

[0002] Ethyl r-4-chloro-3-hydroxybutyrate is an important intermediate for the synthesis of L-carnitine and chiral compounds such as macrolactam A and (R)-γ-amino-β-hydroxybutyric acid (GABOB) body, the market demand is huge.

[0003] The preparation of r-4-chloro-3-hydroxybutyric acid ethyl ester mainly adopts biological methods. At present, the commonly used method is to use microbial methods to prepare r-carbonyl reductase and coenzyme regeneration system, and then use these two enzymes to biocatalyze 4-chloro Ethyl acetoacetate synthesizes ethyl r-4-chloro-3-hydroxybutyrate. Since ethyl r-4-chloro-3-hydroxybutyrate is insoluble in water and has greater toxicity to cells / enzymes, it is generally Water / organic solvent two-phase sys...

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