Hemolytic agent for leukocyte and basophilic granulocyte counting and hemoglobinometry

Abstract

The invention discloses a hemolytic agent for leukocyte and basophilic granulocyte counting and hemoglobinometry, and belongs to the technical field of the blood analysis. The problem that the leukocyte and basophilic granulocyte counting and hemoglobin in the human whole blood cannot be determined at the same time in the prior art is solved. The hemolytic agent is a buffer solution containing cationic surfactant, a nonionic surfactant and hemoglobin stabilizer, wherein the concentration of the cationic surfactant is 0.1g / L to 50g / L, the concentration of the nonionic surfactant is 0.5g / L to 100g / L, the concentration of the hemoglobin stabilizer is 0.1g / L to 50g / L, and a pH value of the buffer solution is 1.8-4.8. The component of hemolytic agent is simplified, the hemolysis time only needs10-18s without using a heating device, namely accomplishing the classified counting on the leukocyte and basophilic granulocyte and the determination on the hemoglobin; the operation is simple and fast and high in accuracy.

Description

technical field [0001] The invention relates to a hemolytic agent, in particular to a hemolytic agent used for counting white blood cells and basophils and measuring hemoglobin, and belongs to the technical field of blood analysis. Background technique [0002] Basophils are the granulocytes that account for the least proportion in human whole blood, and account for 0% to 1% of the total number of white blood cells in the blood of healthy people. Clinically, myeloproliferative diseases, chronic myelogenous leukemia, The number of basophils will change significantly in diseases such as infection and certain food allergies, so the accurate classification and counting of basophils in human whole blood has direct guiding significance for the correct diagnosis of these diseases. [0003] At present, the methods for accurate classification and counting of basophils in human whole blood mainly include impedance method and optical method. When adopting these two kinds of methods to...

AI Technical Summary

Problems solved by technology

When adopting these two kinds of methods to measure, all can not measure hemoglobin at the same time, as CN1113241C discloses a kind of in acid medium with polyethylene oxide type nonionic surfactant and a kind of cationic surfactant to basophilic granule The method of measuring cells, the detection system of this method is the laser light scattering method, and the hemoglobin cannot be measured at the same time

Another example is US5196346, which discloses a reagent that measures basophils by protecting basophils with an electrical impedance method, but this reagent cannot measure hemoglobin, and has high requirements for operating temperature, slow hemolysis speed, and is not easy to use.

In addition, when measured by impedance method, large platelets and Plasmodium will affect the count and classification of white blood cells and basophils

Another example is that US3874852 and US3854914 describe a cyanide reagent of the same type, but the main disadvantage of this reagent is that it contains cyanide and cannot classify and count leukocyte subpopulations in blood samples

US5242832 also describes a similar leukocyte differential reagent, but this reagent cannot be used to identify basophils and can only analyze lymphocytes, monocytes and granulocytes

Therefore, when measuring white blood cells, basophil counts and hemoglobin in human whole blood at the same time, it is necessary to use more than two kinds of reagents at the same time, such as various hemolysins used in conjunction with the SYSMEX XS-800i hemocytometer, reagent components Many, and the operation is complicated

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