Method for measuring content of exogenous KGN in articular cavity

A measurement method and joint cavity technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of simple measurement method, good specificity and low cost

Inactive Publication Date: 2019-07-12
GUANGXI MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no relevant literature report on the detection method of KGN in synovial fluid and articular cartilage.

Method used

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  • Method for measuring content of exogenous KGN in articular cavity
  • Method for measuring content of exogenous KGN in articular cavity
  • Method for measuring content of exogenous KGN in articular cavity

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Embodiment Construction

[0018] The principles and features of the present invention are described below in conjunction with the accompanying drawings, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0019] The KGN standard product used in this example was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd., its production batch number is 1802066, and its high-performance liquid chromatography (HPLC) measured purity ≥ 98%. Twelve SD adult male rats: The weight is about 160-200g, provided by the Animal Experiment Center of Guangxi Medical University (experimental animals have passed the review of the ethics committee), and the human synovial fluid is provided by the First Affiliated Hospital of Guangxi Medical University.

[0020] 1. Configuration of standard stock solution

[0021] Accurately weigh 10mg KGN standard, place it in a 10mL volumetric flask, add 1-3mL DMSO to dissolve, preferably 1mL, di...

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Abstract

The invention discloses a method for measuring the content of exogenous KGN in an articular cavity. The method comprises the following steps: step S1: preparation of a KGN standard stock solution, step S2: joint sampling, step S3: preparation of a standard curve gradient solution, and step S4: measurement of a standard curve and measurement of the content of KGN in the articular cavity of a KGN medication model: preparing the standard curve gradient solution by using synovial fluid or grinding fluid of articular cartilage tissues of a KGN standard product and a KGN non-medication model, so asto draw the standard curve in an HPLC system, wherein a peak area is used as the abscissa of the standard curve, concentration is used as the vertical coordinate of the standard curve, measuring thepeak area obtained by the KGN in the synovial fluid or the articular cartilage tissues of the KGN medication model by using the HPLC system under the same conditions, and then taking the vertical coordinate of the standard curve corresponding to the peak area as the content of the KGN, meanwhile, the measurement method is simple, convenient, fast, time-saving, low in cost, high in accuracy and good in specificity.

Description

technical field [0001] The invention relates to a method for measuring the content of KGN, in particular to a method for measuring the content of exogenous KGN inside and outside the joint cavity of a KGN medication model. Background technique [0002] Kartogenin (KGN) is a synthetic small molecular heterocyclic compound discovered in recent years, which can induce endogenous mesenchymal stem cells (MSCs) to differentiate into chondrocytes. It was first discovered in the study of cartilage repair in osteoarthritis of. KGN's IUPAC name is 2-[((1,1'-Biphenyl)-4-ylamino)carbonyl]benzoic acid, the molecular formula is C 20 h 15 NO 3 1 / 4H 2 O, soluble in DMSO, is off-white solid, odorless or almost odorless, and has not been recorded in the Pharmacopoeia of the People's Republic of China. Since bone and joints lack obvious cartilage vascular tissue, drugs in synovial fluid and articular cartilage tissue generally enter the body through the lymphatic circulation, and KGN is m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/884
Inventor 雷丹青陈海敏王则涛
Owner GUANGXI MEDICAL UNIVERSITY
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