Method for the determination of benzo[a]pyrene in cigarette smoke with matrix-matched solvent calibration

Abstract

The invention discloses a method for calibrating and measuring the content of benzo[a]pyrene in mainstream cigarette smoke based on a matrix matching solvent, including preparation of an internal standard solution, preparation of a matrix matching solvent, preparation of a series of standard working solutions, preparation of a sample solution, and gas chromatography ‑Tandem mass spectrometry (GC‑MS / MS) analysis and standard working curve establishment and sample result calculation. For the first time, the method was calibrated based on matrix-matched solvents, and GC‑MS / MS was used to measure the release of benzo[a]pyrene in mainstream cigarette smoke. The advantages are: (1) The method of preparing matrix matching standard solution reduces the influence of matrix effect. (2) The sample pretreatment only needs to carry out solvent extraction on the smoke particulate matter, which is simple and practical. Compared with the current standard detection method (GB / T 21130‑2007), steps such as sample purification and concentration are omitted, which greatly simplifies the sample processing process and reduces the use of organic solvents and consumables; (3) The target object is sensitive, The method can ensure the accuracy of the quantitative analysis results of the target substance in the low-tar cigarette smoke.

Description

technical field [0001] The invention belongs to the technical field of index determination of cigarette mainstream smoke, and in particular relates to a method for calibrating and measuring the content of benzo[a]pyrene in cigarette mainstream smoke based on matrix matching solvents. Background technique [0002] In recent years, as the public has paid increasing attention to smoking and health issues, the harmful components released in cigarette smoke have also become the focus of attention, and the amount of harmful components released has gradually become the basic basis for evaluating the harmfulness of cigarettes. At present, some countries and regions such as Canada and Germany have enacted relevant laws, requiring cigarette manufacturers to provide the release amount of the main harmful components in cigarette smoke. At the same time, based on the needs of sustainable and stable development, scientific research institutions in the tobacco industry, on the basis of sum...

AI Technical Summary

Problems solved by technology

Compared with the measures taken by the existing standard methods, in order to simplify the sample pretreatment process, if the extraction solution (without purification treatment) is used to directly enter the instrument for analysis, the recovery rate of the target substance in the smoke is low, especially for low-tar cigarettes ( Especially in recent years popular slim cigarettes) quantitative analysis of B[a]P release in mainstream smoke will have an impact

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