application of uridine diphosphate glucuronosyl transferase 1A1 subtype to DON (deoxynivalenol) metabolism
A technology of uridine diphosphate glucose and transferase, applied in the field of molecular biology
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[0042] 1. Materials
[0043] 1.1 Pig liver cells
[0044] Pig liver cells were obtained from one-week-old Panama miniature piglets, provided by the Zhuanghang Experimental Station of Shanghai Academy of Agricultural Sciences.
[0045] 1.2 Materials
[0046] DON standard products were purchased from Romer Company; collagenase IV, DMEM medium, calf serum, trypsin digestion solution, penicillin and streptomycin were purchased from American GIBCO Company; Dulbecco's phosphate buffer, Dulbecco's Hanks' balanced salt and Taiwan Pan blue was purchased from Sigma; Trizol reagent was purchased from Invitrogen; restriction enzymes, T4 DNA ligase, and fluorescent quantitative PCR kit (SYBR Green Premix Ex Taq) were purchased from TaKaRa; fetal bovine serum was purchased from Hangzhou Sijiqing Company ; KOD-plus DNA polymerase was purchased from TOYOBO Company; plasmid extraction kit and gel recovery kit were purchased from Tiangen Biochemical Technology Co., Ltd.; mouse-derived anti-Hi...
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