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application of uridine diphosphate glucuronosyl transferase 1A1 subtype to DON (deoxynivalenol) metabolism

A technology of uridine diphosphate glucose and transferase, applied in the field of molecular biology

Active Publication Date: 2019-07-26
SHANGHAI ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Benefits of technology

This patented technology helps researchers study how dietary fats are broken down by ruminant bacteria called Actinosynnema californicae (a type of gram-positive rods). It suggests that certain chemicals found within these food may help humans breakdown their own fat or reduce harmful substances from them. By measuring this process it allows scientists to better predict what animal's body will absorb more nutrients during different stages of life than usual without having to actually consume any excessive amounts of those products themselves.

Problems solved by technology

Technological Problem addressed in this patents relating to managing industrial wastewater from various sources like fish farms, dairy industry, sheep industries, and crops during their use due to these factors. Current methods involve monitoring changes made over time in biochemistry processes associated with heavy metal accumulations within the bodies' bloodstreams, making them difficult to identify potential hazards. To address this problem, new techniques involving studying specific chemical structures called uranium dioxydehydes(UFOX).

Method used

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  • application of uridine diphosphate glucuronosyl transferase 1A1 subtype to DON (deoxynivalenol) metabolism
  • application of uridine diphosphate glucuronosyl transferase 1A1 subtype to DON (deoxynivalenol) metabolism
  • application of uridine diphosphate glucuronosyl transferase 1A1 subtype to DON (deoxynivalenol) metabolism

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Embodiment 1

[0042] 1. Materials

[0043] 1.1 Pig liver cells

[0044] Pig liver cells were obtained from one-week-old Panama miniature piglets, provided by the Zhuanghang Experimental Station of Shanghai Academy of Agricultural Sciences.

[0045] 1.2 Materials

[0046] DON standard products were purchased from Romer Company; collagenase IV, DMEM medium, calf serum, trypsin digestion solution, penicillin and streptomycin were purchased from American GIBCO Company; Dulbecco's phosphate buffer, Dulbecco's Hanks' balanced salt and Taiwan Pan blue was purchased from Sigma; Trizol reagent was purchased from Invitrogen; restriction enzymes, T4 DNA ligase, and fluorescent quantitative PCR kit (SYBR Green Premix Ex Taq) were purchased from TaKaRa; fetal bovine serum was purchased from Hangzhou Sijiqing Company ; KOD-plus DNA polymerase was purchased from TOYOBO Company; plasmid extraction kit and gel recovery kit were purchased from Tiangen Biochemical Technology Co., Ltd.; mouse-derived anti-Hi...

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Abstract

The invention discloses application of a uridine diphosphate glucuronosyl transferase 1A1 subtype (UGT 1A1) gene to preparing detoxicants for promoting DON metabolism of animals and belongs to the field of molecular biology. Therefore, the invention provides a key regulation factors of liver metabolism of DON, provides a scientific basis for exposure assessment of DON in feeds and also provides aconvenient and effective index for DON metabolism assessment of animals. The invention also discloses a method for assessing DON metabolism of animals by detecting the transcriptional level of the UGT1A1. The method for assessing DON metabolism of animals by detecting the transcriptional level of the UGT 1A1 can accurately, conveniently and rapidly complete assessment. The application of the UGT1A1 to preparing the detoxicants for animals is of high significance to development of new detoxicants for promoting excretion, reducing residues and reducing DON toxicity.

Description

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Claims

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Application Information

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Owner SHANGHAI ACAD OF AGRI SCI
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