Dual-channel dual-wavelength phase microscopy imaging system and method under non-orthogonal basis

Abstract

The invention provides a dual-channel dual-wavelength phase microscopic imaging system and method under a non-orthogonal basis, including a light source, a 2f mirror, a beam splitting aperture diaphragm, a beam expander collimating mirror, a sample platform, an objective lens, a first channel, a second Two channels and computer; the first channel and the second channel are placed side by side; the first channel includes the first diaphragm, the first collimating mirror, the first grating, the first Fourier lens, the first spatial light modulator, the second Fourier lens and the first CCD camera; the structure of the second channel is the same as that of the first channel; the light beam emitted by the light source is divided into two non-orthogonal parallel beams after passing through the 2f mirror, the beam splitter aperture diaphragm, and the beam expander collimator in turn , and then pass through the sample platform and objective lens in turn, and enter the first channel and the second channel respectively to obtain the interference fringe pattern, which is transmitted to the computer for pattern processing to obtain the three-dimensional shape of the sample. The present invention can perform one-time synchronous phase microscopic imaging of dual-wavelength and dual-path light, obtain the spatial phase distribution of the phase body according to the interference micrograph, and use the reconstruction algorithm to obtain the three-dimensional shape of the sample, and is especially suitable for biological cells Morphological Transient Microscopy.

Description

technical field [0001] The invention belongs to the technical field of phase microscopic imaging, and in particular relates to a dual-channel dual-wavelength phase microscopic imaging system and method under a non-orthogonal basis. Background technique [0002] Light microscopy is a vital tool in the fields of biology and medicine because most cells and tissues are "transparent" under the microscope and have traditionally been made "visible" by staining them. A series of technological innovations in recent years have broken through the past limitations of phase microscopy, which has led to the emergence of quantitative imaging microscopy (QPI). QPI operates on unlabeled samples, thus, it is a complement to established fluorescence microscopy with low phototoxicity and no photobleaching. Since the image represents a quantitative map of the optical path length delay introduced by the sample, QPI provides an objective measure of morphology and kinetics independent of contrast ...

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