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A method for correcting the influence of hemolysis on the detection of red blood cell series parameters

A technology for parameter detection and red blood cell count, which is applied in the field of correcting the influence of hemolysis on the detection of red blood cell series parameters, and the field of correcting the influence of hemolysis on the measurement of red blood cell count and related parameters. and other problems, to achieve the effect of simple operation, high accuracy and time saving

Active Publication Date: 2020-09-01
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It not only increases the physical burden and mental stress of patients, wastes testing resources, but also may affect clinical diagnosis and treatment due to delays in test reports

Method used

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  • A method for correcting the influence of hemolysis on the detection of red blood cell series parameters
  • A method for correcting the influence of hemolysis on the detection of red blood cell series parameters
  • A method for correcting the influence of hemolysis on the detection of red blood cell series parameters

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] EDTAK 2 (Potassium oxalate tetraacetate) anticoagulated (special concentration for blood routine) normal fresh whole blood (no hemolysis, observed with the naked eye, the plasma is light yellow and transparent after erythrocyte sedimentation), 40 parts were divided into 4 groups, 10 parts in each group , 2ml per serving.

[0022] (1) Complete blood cell count for each part of normal fresh whole blood

[0023] When the XN-L 330 hematology analyzer is ready (the green Ready light is on), invert the test tube containing normal fresh whole blood 8-10 times to mix the blood evenly, then remove the cap of the test tube, and place the normal fresh whole blood in the Under the instrument's sampling needle, confirm that the sampling needle enters the middle of the blood, press the green counting button to aspirate the sample, when the instrument's suction sound stops, remove the test tube, and the concentration of red blood cells (RBC) and hemoglobin will be automatically displ...

Embodiment 2

[0033] The venous blood routine samples submitted for clinical examination in a children's hospital for seven consecutive days in June 2018 were selected, and abnormal samples such as blood coagulation and insufficient sample volume were excluded. All experiments were completed on the day of sample collection.

[0034] (1) Perform a complete blood count on all clinical samples

[0035] All clinical EDTA-K 2 The anticoagulant venous blood sample is automatically detected by the XN2800 blood analyzer, and the test tubes containing the whole blood sample are placed on the special test tube rack in sequence. When the instrument is ready (the green Ready light is on), the test tube rack is placed in the sampling area. The instrument automatically injects, mixes, sucks and completes the test, and automatically displays red blood cells (RBC), hemoglobin concentration (HGB), hematocrit (HCT), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) meas...

Embodiment 3

[0062] Selected 15 discarded venous whole blood samples from a children's hospital on a certain day in June 2018 for clinical testing, no hemolysis (observed with the naked eye, the plasma was pale yellow and transparent after erythrocyte sedimentation).

[0063] (1) Perform a complete blood count on each clinical sample

[0064] EDTA-K above 2 The anticoagulant venous blood sample is automatically detected by the XN2800 blood analyzer, and the test tubes containing the whole blood sample are placed on the special test tube rack in sequence. When the instrument is ready (the green Ready light is on), the test tube rack is placed in the sampling area. The instrument automatically injects, mixes, sucks and completes the test, and automatically displays red blood cells (RBC), hemoglobin concentration (HGB), hematocrit (HCT), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) measured value.

[0065] (2) In vitro artificial hemolysis of blood ...

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Abstract

The invention provides a method for correcting influence of hemolysis on detection of red blood cell series parameters, and the method comprises the following steps of obtaining a red blood cell countmeasurement value, a hemoglobin concentration measurement value, a blood cell specific product measurement value and an average red blood cell hemoglobin measurement value by complete blood cell count; separating the red blood cells and the plasma to obtain the hemoglobin concentration measurement value in the plasma, obtaining a corresponding correction value, and evaluating the result. The redblood cell series parameters of the hemolyzed blood obtained by the method provided by the invention have high numerical accuracy, and the difference from the true value is less than + / -0.5% on average. The method provided by the invention is simple in operation, and the detecting instrument and centrifuge used are laboratory routine equipment, thereby facilitating the popularization and application in various hospitals. The method provided by the invention requires a short time and can quickly obtain the detection result, that is, saving time and saving cost.

Description

technical field [0001] The invention belongs to the field of biological measurement, relates to the measurement of red blood cells and related parameters of blood, in particular to a method for correcting the influence of hemolysis on the detection of red blood cell series parameters, and is a method for correcting the influence of hemolysis on the determination of red blood cell count and related parameters. Background technique [0002] Hemolysis is a phenomenon in which hemoglobin is released into the plasma after some red blood cells in the blood are destroyed. After hemolysis occurs, the red blood cell count in routine blood tests will be falsely reduced, and related parameters such as mean hemoglobin concentration (MCHC) will be falsely increased. Experiments have shown that plasma free hemoglobin (PHB) can be used to evaluate the degree of hemolysis. When PHB reaches 1.4g / L, it will have a significant impact on the measured values ​​of red blood cells and related para...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/72G01N33/80G01N33/49G01N33/48C12Q1/06
CPCG01N33/48G01N33/49G01N33/721G01N33/80
Inventor 彭朝阳沈红强毛建华舒强项文清周建明李伟蔡锋晴
Owner ZHEJIANG UNIV
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