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Method for using microorganisms to control iron deficiency yellowing of cinnamomum camphora

A technology for microbial control and camphor, which is applied in the field of prevention and control of iron-deficiency yellowing disease of forest trees, and achieves the effects of improving utilization rate, good practicability and promoting iron transport.

Inactive Publication Date: 2019-09-06
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It makes up for the gap in the use of microorganisms to prevent and control iron-deficiency chlorosis of camphor, and will not damage the tree during use, nor will it cause soil and environmental pollution, and has very good practicability

Method used

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  • Method for using microorganisms to control iron deficiency yellowing of cinnamomum camphora
  • Method for using microorganisms to control iron deficiency yellowing of cinnamomum camphora
  • Method for using microorganisms to control iron deficiency yellowing of cinnamomum camphora

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Preparation of siderophilic fermented broth of Lahnsia waterus JZ-GX1: 1) Pick a single colony from a solid NA plate, pick up a fresh bacterial lawn with a platinum wire inoculation loop and put it in 50mL NB medium, 28°C, 180r / min Cultivate overnight, then centrifuge (4°C, 10,000r / min, 10min) to collect the bacteria, wash twice with 0.85% normal saline, adjust the concentration of the bacteria suspension to 10 7 cfu / mL spare. 2) Introduce the bacterial suspension into KMB medium, the initial pH is 8.0, the liquid volume is 25mL / 50mL, inoculate with 1% inoculum, and cultivate at 28°C for 36h to obtain a fermented liquid containing siderophiles.

[0027] by FeCl 3 Experiment, tetrazolium salt experiment, Arnow's experiment and Shenker's experiment were used to detect the siderophilic type of Lahnella waterus JZ-GX1. In Shenker's experiment, the siderophore solution prepared by JZ-GX1 has an absorption peak at 230nm ( figure 1 a), indicating that the bacterium can prod...

Embodiment 2

[0031] The siderophilic fermentation broth of Lahnella water JZ-GX1 prepared in Example 1 was used to prevent and control iron-deficiency chlorosis of Cinnamomum camphora. The data in this example were statistically analyzed using EXCLE 2010 and SPSS 23.0 software, and the significance of the difference was analyzed using Duncan's new multiple range test (different lowercase letters on the histogram of the attached figure represent significant differences at the 0.05 level).

[0032]The camphor growth soil of the present embodiment is collected from the B layer soil of Beida Mountain, Nanjing Forestry University, and the parameters of the soil properties are measured as follows: pH7.6, organic matter content 30.1mg / kg, alkaline hydrolyzed nitrogen 1.92mg / kg, available phosphorus 0.24mg / kg, available potassium 499.92mg / kg.

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Abstract

The invention discloses a method for using microorganisms to control iron deficiency yellowing of cinnamomum camphora and belongs to the technical field of controlling iron deficiency yellowing diseases in trees. The method includes applying plant growth promoting rhizobacteria (PGPR), rahnella aquatilis JZ-G 1 ferritin fermentation broth into the root of a cinnamomum camphora tree to improve irondeficiency yellowing of the cinnamomum camphora. The PGPR, rahnella aquatilis JZ-GX1 ferrtin fermentation broth is applied to the control of iron deficiency yellowing of the cinnamomum camphora to promote iron transport, and multiple roles are played in improving plant iron nutrition; the PGPR, rahnella aquatilis JZ-GX1 ferrtin fermentation broth applied to alkaline or nutrient-poor soil as an important improvement measure to alleviate the symptoms of iron deficiency and chlorosis, and a broad application prospect is achieved.

Description

technical field [0001] The invention belongs to the technical field of prevention and treatment of iron-deficiency chlorosis of forest trees, and in particular relates to a method for preventing and treating iron-deficiency chlorosis of camphor camphor by using microorganisms. Background technique [0002] Iron (Fe) is an essential micronutrient element for plants. Although the total iron content is abundant in most soils, the soluble iron content that can be absorbed and utilized by plants is very low. Because the solubility of iron is greatly affected by the pH value, in alkaline or calcareous soil (pH7.4-8.5), the soluble iron content that can be absorbed and utilized by plants is only 10 -11 -10 -10 mol / L, far from meeting the normal growth of plants (10 -6 -10 -8 mol / L) needs. In addition, the increasingly serious soil salinization and excessive application of chemical fertilizers will further reduce the content of soluble iron in the soil. Therefore, iron deficienc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G17/00A01G7/06C12P13/04C12R1/01
CPCA01G7/06A01G17/005C12P13/04
Inventor 吴小芹孔维亮叶建仁赵银娟
Owner NANJING FORESTRY UNIV
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