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Tobacco heat shock protein hsp22 and its application

A heat shock protein and tobacco technology, applied in the field of tobacco genetic engineering, can solve the problems of poor appearance quality, affecting the grade and quality of tobacco, producing harmful substances, etc.

Active Publication Date: 2022-05-31
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

"Green-yellow smoke" not only has poor appearance, but also produces harmful substances when burned, which in turn affects the grade and quality of tobacco

Method used

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  • Tobacco heat shock protein hsp22 and its application
  • Tobacco heat shock protein hsp22 and its application
  • Tobacco heat shock protein hsp22 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The present embodiment is briefly described as follows with respect to the construction process of tobacco NtHSP22 gene cloning and silencing vector.

[0037] The PCR amplification program is: 95°C pre-denaturation for 3 min; 95°C denaturation for 15s, 55°C annealing for 15s, 72°C extension for 30s, 34°C

[0038] The PCR amplification product was detected by agarose gel electrophoresis, and the electrophoresis product was recovered for subsequent use.

Embodiment 2

~

1.5 or so, then centrifuge at 4000g for 5 min, collect the bacteria, and then use MMA (1 mL (1 M) MgCl

2

; 1 mL

(1 M, pH 5.6) MES; 75 μL (200 mM) As), resuspend, adjust OD

600

= about 1.0;

[0056] After the final room temperature was placed for about 3 h, it was used as a bacterial solution for transfection.

(3) instantaneous conversion

With 3

[0059] The tobacco phenotype changes after injection for 3 weeks are shown in Figure 1. It can be seen that Agrobacterium infection with TRV2-PDS

The leaves of N. benthamiana at 4 w seedling age were used as the experimental material. Using a 1 mL syringe, the

The bacterial solution for transfection was prepared and injected into tobacco leaves, and the injected tobacco was continued to be cultured in an artificial incubator to observe phenotypic changes.

change.

[0063].

The new leaves of the plant had bleaching phenomenon, indicating that the infection was successful; while there was no significant change in ...

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Abstract

The invention belongs to the field of tobacco genetic engineering, and in particular relates to a tobacco heat shock protein HSP 22 gene and a patent application for its application. The base sequence of the gene is shown in SEQ ID NO.1. Tobacco heat shock protein HSP 22 consists of 192 amino acid residues, of which amino acid 68‑159 is a conserved SHSP domain. This protein is related to the content of pigment substances in plant leaves. After reducing the expression of this protein, the content of pigment substances in leaves is significantly reduced. The pigment substances are: neoxanthin, violaxanthin, lutein, chlorophyll a / b ,β-carotene. Through preliminary research on the specific tobacco heat shock protein HSP 22, the present invention finds that it is highly correlated with the content of tobacco pigments, and after the gene is silenced, the content of pigments in tobacco is significantly reduced. Based on this characteristic, it can provide a certain application basis and reference for the cultivation of new tobacco varieties.

Description

Tobacco heat shock protein HSP22 and its application technical field The invention belongs to the field of tobacco genetic engineering, be specifically related to tobacco heat shock protein HSP 22 and application patent application thereof Please. Background technique [0002] Nicotiana, which people usually smoke, belongs to the Dicotyledonous plant class Anchiaceae Solanaceae Nicotiana, Nicotiana There are more than 60 species of (Nicotiana), and the tobacco used for the preparation of smoking is mainly two cultivars, the common tobacco (also known as Nicotiana tabacum) and Nicotiana rustica, of which the former dominates However, the cultivation area of ​​the latter is relatively small. Cultivated tobacco can be divided into flue-cured tobacco, sun-cured tobacco, There are six types of air-cured tobacco, burley tobacco, oriental tobacco and yellow-flowered tobacco, among which flue-cured tobacco is the most widely cultivated common tobacco. Flue-cured tobacco p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/82C12N15/11C12N15/10C07K14/415A01H5/00A01H6/82
CPCC07K14/415C12N15/825
Inventor 陈千思王晨周会娜刘萍萍李泽锋郑庆霞金静静徐国云魏攀
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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