Plant non-solid tissue culture medium and plant non-solid tissue culture method

Abstract

The invention discloses a plant non-solid tissue culture medium and a plant non-solid tissue culture method, wherein agar, agarose or carrageenan is added into the non-solid culture medium, the concentration of the agar is 1-5.5g / L, the concentration of the agarose is 1-5g / L, and the concentration of the carrageenan is 1.5-6g / L; the plant non-solid tissue culture method comprises the following steps: (1) selection and obtaining of an explant; (2) preparation of a non-solid medium, which relates to subpackaging the medium into a tissue culture container, sterilizing at high temperature and highpressure, cooling, then opening the bottle cap of a tissue container, and then enabling supports such as filter paper, non-woven fabrics and sponge, which are subjected to high-temperature high-pressure sterilization, to be tiled on a liquid surface; and (3) culturing. Based on current fluid media components, the agar, the agarose or the carrageenan is added into the plant non-solid tissue culture medium, wherein the concentration of the agar is 1-5.5g / L, the concentration of the agarose is 1-5g / L, the concentration of the carrageenan is 1.5-6g / L, so that the medium possesses a certain supporting capacity and has a certain supporting capacity to a support in the plant non-solid tissue culture process, the supporting capacity of the support to the explant is further improved, the explant is prevented from being dipped dead, and the plant non-solid tissue culture medium is convenient for industrialized promotion.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a plant non-solid tissue culture medium and a plant non-solid tissue culture method. Background technique [0002] At present, most of the tissue culture factories adopt the plant solid tissue culture method, the cultivation cost is high, the growth rate of the bottle seedlings is slow, and the root system of the bottle seedlings is difficult to clean and easily damaged. According to relevant literature and patent reports, the existing non-solid tissue culture method mainly adopts shallow layer liquid culture, which requires shaking culture. In addition, there are also patent reports that soak the sponge in the nutrient solution, and then soak the sponge with the nutrient solution. Putting it into a bottle for culturing the explants, the plant is prone to lack of nutrition, and the bottle needs to be opened halfway to replenish the liquid. The operation is complicated ...

AI Technical Summary

Problems solved by technology

[0002] At present, most of the tissue culture factories adopt the method of plant solid tissue culture, the cultivation cost is high, the growth rate of the bottle seedlings is slow, and the root system of the bottle seedlings is difficult to clean and easily damaged

According to relevant literature and patent reports, the existing non-solid tissue culture method mainly adopts shallow layer liquid culture, which requires shaking culture. In addition, there are also patent reports that soak the sponge in the nutrient solution, and then soak the sponge with the nutrient solution. Cultivate explants in bottles. This method is easy for plants to lack nutrients, and the bottle needs to be opened to replenish fluids in the middle. The operation is complicated and the risk of pollution is high, which is not convenient for industrialization.

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