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Method for breeding meloidogyne hapla by using eupatorium adenophora Spreng.

A technology of Eupatorium adenophorum and root-knot nematodes, which is applied to the field of cultivating root-knot nematodes, achieving the effects of easy planting, strong vitality and adaptability, and low cost

Active Publication Date: 2019-09-20
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far there is no method for cultivating M. borealis using Eupatorium adenophorum

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Select healthy plants of Eupatorium adenophorum that were born in the current year, the growth time is ≤2 months, the plant height is ≤1.0m, and the root system length is more than 10cm. Clean the root system and plants with tap water, and place them in a ventilated place. The upper part of the plant is about 1 / 3 of the height, the main bud is removed, and the remaining 2 / 3 of the leaves are reserved for future use.

[0026] Take soil suitable for planting and dry it at 100°C for 2 hours to kill other nematodes and eggs that may be contained in the soil, return to normal temperature and lay evenly in a container with good air permeability, with a soil thickness of about 1.8cm for later use. Get the vermiculite of 5mm diameter particle and carry out sterilization for later use.

[0027] Spread the prepared root system of Eupatorium adenophorum plant on sterile neutral filter paper, moisten the filter paper with distilled water, it is advisable that there is no visible wa...

Embodiment 2

[0029] The root system of the Eupatorium adenophorum plant was prepared according to the method in Example 1, and the root system was spread on sterile neutral filter paper, and the filter paper was moistened with distilled water, preferably without visible water under the filter paper. The purchased root-knot nematode oocysts were inoculated on the roots of Eupatorium adenophorum with a diameter of more than 1.0 mm, and 8 oocysts were inoculated per 1.0 cm root. After inoculation, cover the surface of the root system with moistened absorbent cotton (subject to the fact that no water can be squeezed by hand), transfer it to an artificial climate box with a temperature of 36°C and a relative humidity of 60% for 24 hours under light conditions, and lay the root system at the end of the plant after taking it out On the surface of the soil prepared by the method of Example 1, try not to bury the inoculation site in the soil, fill in the vermiculite prepared by the method of Example...

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Abstract

The invention discloses a method for breeding meloidogyne hapla by using eupatorium adenophora Spreng., and belongs to the technical field of biology. The method comprises the steps of collecting or purchasing the meloidogyne hapla or oocysts for cold storage for use, preparing a culture material of the eupatorium adenophora Spreng., preparing a planting medium, and conducting inoculation. The method has the advantages that the eupatorium adenophora Spreng. is high in vitality and adaptability, capable of surviving easily, easy to plant, insensitive to most kinds of meloidogyne spp. and high in specific correspondence to the meloidogyne hapla, and the single meloidogyne hapla is easily obtained; the cost of breeding the meloidogyne spp. by the eupatorium adenophora Spreng. is low; compared with other crops, the breeding cycle is longer, and living bodies of the meloidogyne hapla can be continuously obtained; repeated use can be achieved after one-time inoculation.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a method for cultivating root-knot nematode borealis. Background technique [0002] Root-knot nematode (Meloidogyne spp.) is a highly specialized omnivorous plant pathogenic nematode, and northern root-knot nematode (Meloidogyne hapla) is one of the main nematodes that attack crops. The root-knot nematode mainly parasitizes crops and causes tumors. In plots with high insect population density, many tumors are formed on the roots. In severe cases, the roots rot and fall off, resulting in yellowing of leaves, stop of growth, wilting and death of plants, especially serious damage to false planting beds and seedbeds of long runners. Meloidogyne borealis has a wide range of harms. Except for a few crops, it almost invades all dry crops, especially the annual Solanaceae crops and annual Cruciferae crops. In recent years, root-knot nematode disease has also become one ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033A01G22/00
CPCA01K67/033A01G22/00
Inventor 吴国星何霞红王扬朱书生高熹朱有勇王柱华刘晓慧兰明先
Owner YUNNAN AGRICULTURAL UNIVERSITY
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