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Method for extracting 2-hydroxybenzylamine from tartary buckwheat

A technology of hydroxybenzylamine and tartary buckwheat, which is applied in the field of extracting 2-hydroxybenzylamine, and achieves the effect of low raw material cost

Active Publication Date: 2022-02-11
北京浩鼎瑞健康科技中心(有限合伙)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, in the prior art, 2-hydroxybenzylamine is usually prepared by the method of raw material synthesis, and there is no report of extracting 2-hydroxybenzylamine from plant raw materials.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method for extracting 2-hydroxybenzylamine from tartary buckwheat, specifically comprising the following steps:

[0033] (1) Dry tartary buckwheat root, stem and grain respectively, add liquid nitrogen and grind to obtain powder;

[0034] (2) Mix the powder with deionized water, adjust the pH value of the solution to 5.0, then add 0.5% powder weight mixed enzyme for enzymolysis, the enzymolysis temperature is 25°C, and the enzymolysis time is 40min. After the enzymolysis is completed, filter , to obtain the filter residue; wherein, the mixed enzyme is a mixture of cellulase and pectinase in a weight ratio of 2:1;

[0035](3) The filter residue obtained in step (2) is mixed with 95% ethanol at a solid-to-liquid ratio of 1g:10mL, and then the ultrasonic power density is 100W / cm 2 1. Ultrasonic extraction was carried out under the condition of ultrasonic frequency of 30kHz, the extraction temperature was 20°C, the extraction time was 30min, the extraction was repeated 3...

Embodiment 2

[0045] A method for extracting 2-hydroxybenzylamine from tartary buckwheat, specifically comprising the following steps:

[0046] (1) drying tartary buckwheat roots, stems and grains respectively, adding liquid nitrogen and grinding to obtain powder;

[0047] (2) Mix the powder with deionized water, adjust the pH value of the solution to 6.0, then add 0.8% powder weight mixed enzyme for enzymolysis, the enzymolysis temperature is 50°C, the enzymolysis time is 30min, and filter after the enzymolysis is completed , get filter residue; Wherein, mixed enzyme is the mixture that cellulase and pectinase are mixed in 5:1 by weight ratio;

[0048] (3) The filter residue obtained in step (2) is mixed with 95% ethanol at a solid-to-liquid ratio of 1g:20mL, and then the ultrasonic power density is 150W / cm 2 1. Ultrasonic extraction was carried out under the condition of ultrasonic frequency of 50kHz, the extraction temperature was 40°C, the extraction time was 20min, the extraction was ...

Embodiment 3

[0058] A method for extracting 2-hydroxybenzylamine from tartary buckwheat, specifically comprising the following steps:

[0059] (1) drying tartary buckwheat roots, stems and grains respectively, adding liquid nitrogen and grinding to obtain powder;

[0060] (2) Mix the powder with deionized water, adjust the pH value of the solution to 5.5, then add 0.6% powder weight mixed enzyme for enzymolysis, the enzymolysis temperature is 35°C, and the enzymolysis time is 35min. After the enzymolysis is completed, filter , to obtain the filter residue; wherein, the mixed enzyme is a mixture of cellulase and pectinase in a weight ratio of 3:1;

[0061] (3) the filter residue obtained in step (2) is mixed with 95% ethanol at a solid-to-liquid ratio of 1g:13mL, and then the ultrasonic power density is 120W / cm 2 1. Ultrasonic extraction was carried out under the condition of ultrasonic frequency of 35kHz, the extraction temperature was 25°C, the extraction time was 15min, the extraction w...

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Abstract

The invention discloses a method for extracting 2-hydroxybenzylamine from tartary buckwheat, which comprises the following steps: using tartary buckwheat roots, stems and grains as raw materials, sequentially performing liquid nitrogen grinding, enzymatic hydrolysis treatment, ultrasonic extraction, and alkaline solution extraction , acidify the lye with hydrochloric acid, adsorb it with NDA-150 resin, elute with different lye and water, and finally recrystallize to obtain 2-hydroxybenzylamine. The method uses tartary buckwheat roots, stems and grains as raw materials, and provides a new raw material and extraction method for the extraction process of 2-hydroxybenzylamine. The cost of raw materials is low, and the yield of 2-hydroxybenzylamine extracted by the extraction method is High, high purity, suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of active ingredient extraction, and in particular relates to a method for extracting 2-hydroxybenzylamine from tartary buckwheat. Background technique [0002] 2-Hydroxybenzylamine (2-HOBA), also known as salicylamine, is a potent γKA scavenger that clears γKAs 980 times faster than the formation of γKA protein adducts, thus protecting cells Protection from deleterious effects of γKA adducts. In vitro studies have demonstrated that 2-HOBA protects HepG2 cells from H 2 o 2 The effect of inducing cytotoxicity; the beneficial effect of 2-HOBA in oxidative stress-related diseases has also been found in multiple organs and systems in the body. 2-HOBA administration dose-dependently reduced biomarkers of oxidant damage and prolonged lifespan in C. elegans. Beneficial effects of 2-HOBA have been observed in various mouse models of neuropathology. After oral administration, 2-HOBA can pass through the blood-br...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C213/10C07C215/50C07C231/24C07C235/60
CPCC07C213/10C07C231/24C07C215/50C07C235/60
Inventor 蔡光泽刘新宇夏燕莉
Owner 北京浩鼎瑞健康科技中心(有限合伙)
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