Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Chemical method detection test paper for stool occult blood and preparation method of chemical method detection test paper

A technology of detection test paper and chemical method, which is applied in the direction of biological testing, analysis through chemical reaction of materials, material analysis through observation of the influence of chemical indicators, etc., can solve problems such as wide linear range, achieve low cost, Easy production and processing, and the effect of improving detection efficiency

Inactive Publication Date: 2019-09-24
ZHUHAI KEYU BIOLOGICAL ENG
View PDF9 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the sensitivity of the chemical method is low, its linear range is wide. The color depth of the chemical method is proportional to the Hb content and is not affected by the amount of bleeding. irreplaceable

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Chemical method detection test paper for stool occult blood and preparation method of chemical method detection test paper
  • Chemical method detection test paper for stool occult blood and preparation method of chemical method detection test paper

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0028] like figure 2 , the preparation method of the chemical method detection test paper of a kind of fecal occult blood described in the embodiment of the present invention, comprises the following steps in turn:

[0029] Step S1, after fully immersing the filter paper in the first immersion liquid, take it out, dry it, and form a solidified layer of the first immersion liquid on the surface of the filter paper, the first immersion liquid is composed of buffering agent, lemon yellow, surfactant, chelating agent and water. Specifically, it may be as follows: fully immerse the filter paper in the first immersion liquid, take it out, and dry it at a temperature of 50-90°C for 30-60 minutes to form a solidified layer of the first immersion liquid on the surface of the filter paper; wherein the first immersion liquid It is made of 45-60g buffer, 0.6-1.0g tartrazine, 10-15g surfactant, 1.0-3.0g chelating agent, and 1000g water, and the pH of the first soaking solution is 7-10. Wh...

Embodiment 1

[0034] This embodiment provides a chemical detection test paper for fecal occult blood, which is prepared by the following steps:

[0035] (1) configure the first soaking liquid: put 20g maleic acid and 40g tris, 0.8g tartrazine, 12g surfactant, 2g ethylenediaminetetraacetic acid disodium salt into the container, Add water to 1000g, mix well; the pH of the first dipping solution is 9 after testing with pH test paper;

[0036] (2) After fully immersing the filter paper in the first immersion liquid, take it out, and carry out the first drying at 70°C for 50 minutes to form a solidified layer of the first immersion liquid on the surface of the filter paper;

[0037] (3) Configure the second dipping solution: dissolve 1.5g of tetramethylbenzidine, 7g of cumene hydroperoxide, 50g of surfactant, and 1.2g of 4-hydroxyquinoline in 1L of ethanol;

[0038] (4) Immerse the filter paper that formed the first immersion solidified layer after the first drying into the second immersion liq...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to chemical method detection test paper for stool occult blood and a preparation method of the chemical method detection test paper. The test paper comprises filter paper; the surface of the filter paper is sequentially provided with a first immersion liquid solidified layer and a second immersion liquid solidified layer from inside to outside; a mixed liquid composed of a buffering agent, lemon yellow, a surfactant, a chelating agent and water is cured, so that the first immersion liquid solidified layer can be obtained; and tetramethyl benzidine, cumyl hydroperoxide, a surfactant and a catalyst are dissolved in an organic solvent so as to form a liquid, and the liquid is solidified, so that the second immersion liquid solidified layer can be formed. Therefore, the test paper can rapidly react with hemoglobin in excrement rapidly. The test paper has the advantages of high detection efficiency, obvious color gradations and easiness in visually identifying and judging the rough amount of the bleeding of a patient. The test paper can correctly guide clinical medication. The test paper is prepared through a two-step immersion liquid method; the consumption of the surfactant is low; sensitivity is high; a result can be measured rapidly and accurately; production and processing are easy; and cost is low.

Description

technical field [0001] The invention belongs to the technical field of feces detection, in particular to a test paper for chemical detection of fecal occult blood and a preparation method thereof. Background technique [0002] Fecal occult blood is an early warning of gastrointestinal abnormalities. When the amount of gastrointestinal bleeding is small, the appearance of feces may not change abnormally and cannot be recognized by naked eyes. Therefore, for patients suspected of chronic gastrointestinal bleeding, fecal occult blood examination should be performed, which is of great significance for early screening of gastrointestinal malignant tumors (such as gastric cancer, colorectal cancer, polyps, and adenomas). [0003] At present, the commonly used methods for detecting fecal occult blood are mainly chemical method and immunological method. The immunoassay for the determination of hemoglobin monoclonal antibody has high sensitivity and strong specificity, but it also h...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N33/52
CPCG01N21/78G01N33/526G01N2021/7759
Inventor 孙立平郭亚
Owner ZHUHAI KEYU BIOLOGICAL ENG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com