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Composition for inhibiting cancer metastasis and treating cancer

A composition and technology for inhibiting cancer, which can be used in drug combinations, medical preparations containing active ingredients, applications, etc., and can solve the problems of lack of cancer cell proliferation and metastatic drugs, etc.

Active Publication Date: 2019-09-27
ONCOCROSS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Also, current treatments focus on the apoptosis or removal of cancer cells, and thus, there is a lack of research on drugs for the proliferation and metastasis of cancer cells that directly affect the survival rate of cancer patients

Method used

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  • Composition for inhibiting cancer metastasis and treating cancer
  • Composition for inhibiting cancer metastasis and treating cancer
  • Composition for inhibiting cancer metastasis and treating cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0132] Example 1. Confirmation of anticancer and metastasis inhibitory effects on colorectal cancer

[0133] 1-1. Confirmation of cell viability

[0134] 1-1-1. Confirmation of cell viability based on individual administration

[0135] To confirm the survival of colorectal cancer cells when chlorphenensin (named OC-201), chloroquine (named OC-202), and chloropyrazine (named OC-203) were administered individually According to the manufacturer's protocol, MTT assay (Promega, Ltd.) was used to evaluate the cell viability of colorectal cancer cell lines CT26, HCT116 and SW480 cell lines. 5 × 10 per well in a 96-well plate 3 The cell density of each colorectal cancer cell line was inoculated, and chlorphenesin (OC-201), chloroquine (OC-202) and chlorpyrazine (OC-203) were respectively treated with OμM (control group DMSO treatment), 10μM, 25μM, Cells pretreated with 50μM, 100μM, 250μM, 500μM and 1mM for 24h, 48h or 72h were incubated with 5mg / mL MTT for 4 hours. Then, the cultu...

Embodiment 2

[0163] Example 2. Confirmation of the effect on pancreatic cancer

[0164] 2-1. Confirmation of cell viability

[0165] 2-1-1. Confirmation of cell viability based on individual administration

[0166] In order to confirm the effects of individual administration of chlorphenesin (OC-201), chloroquine (OC-202) and chlorpyrazine (OC-203) on the survival rate of pancreatic cancer cells, according to the manufacturer's protocol and using MTT assay (Promega, Ltd.) was used to evaluate the cell viability of pancreatic cancer cell lines Aspc-1, MIAPaCA2 and Panc-1. 5 × 10 per well in a 96-well plate 3 The cell density of each pancreatic cancer cell line was inoculated, and the concentration of chlorphenesin (OC-201) was 0 μM (DMSO treatment in the control group), 5 μM, 10 μM, 25 μM, 50 μM, 100 μM, 250 μM, 500 μM and 1 mM (1000 μM) respectively. , Chloroquine (OC-202) was treated with OμM (control group DMSO), 0.5 μM, 1 μM, 5 μM, 10 μM, 25 μM, 50 μM and 100 μM, and chloropyrazine (...

Embodiment 3

[0178] Example 3. Confirmation of the effect on biliary tract cancer

[0179] 3-1. Confirmation of cell viability

[0180] 3-1-1. Confirmation of cell viability based on individual administration

[0181] In order to confirm the effects of chlorphenesin (OC-201), chloroquine (OC-202) and chlorpyrazine (OC-203) on the survival rate of biliary tract cancer cells when administered alone, according to the manufacturer's protocol and using MTT assay (Promega, Ltd.) was used to evaluate the cell viability of biliary tract cancer cell lines SNU1079 and SNU308. 5 × 10 per well in a 96-well plate 3 The cell density of each biliary tract cancer cell line was inoculated, and the concentration of chlorphenesin (OC-201) was 0 μM (DMSO treatment in the control group), 5 μM, 10 μM, 25 μM, 50 μM, 100 μM, 250 μM, 500 μM and 1 mM (1000 μM) respectively. , and chloroquine (OC-202) and chlorpyrazine (OC-203) were pretreated with OμM (control group DMSO treatment), 1μM, 5μM, 10μM, 25μM, 50μM an...

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Abstract

The present invention relates to an anticancer and metastasis inhibiting effect by treatment with chlorphenesin, chloroquine, and chloropyrazine alone or in combination. Chlorphenesin, chloroquine, or chloropyrazine show the effect of killing cancer cells and inhibiting proliferation, and metastasis of cancer cells and particularly, a combination thereof is identified to have synergism. Cancer can be effectively prevented or treated by administering chlorphenesin, chloroquine, and chloropyrazine alone or in combination thereof.

Description

technical field [0001] The present invention relates to a composition for treating cancer and inhibiting cancer metastasis, and relates to the anticancer and cancer metastasis inhibiting effects based on single or combined treatment of chlorphenesin, chloroquine and chlorpyrazine. Background technique [0002] The smallest unit that makes up the human body is called a cell. Normal cells divide and grow through intracellular regulatory functions, and will die and disappear to maintain the balance of cell numbers. When cells are damaged for some reason, they are treated to make the cells function normally or cause them to die if they cannot be restored. However, due to various reasons, if the gene of the cell changes, the cell will not grow, so it will not grow completely, and it will not be able to regulate the cell cycle, so the cell will continue to divide. This phenomenon is called cancer (cancer) . Moreover, cancer not only invades surrounding tissues and organs, but al...

Claims

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Application Information

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IPC IPC(8): A61K31/27A23L33/10A61K45/06
CPCA23L33/10A61P35/00A61K31/085A61K31/4706A61K31/4965A61K2300/00A61K45/06A61K31/27A23V2200/308A23V2002/00
Inventor 金艺让
Owner ONCOCROSS CO LTD