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Biomarkers used for diagnosing pancreatitis and derived from intestinal tract, screening method and application of biomarker

A biomarker and pancreatitis technology, applied in the field of biomarkers for the diagnosis of pancreatitis, can solve the problems of low specificity and unclear pathological basis, and achieve the effect of precise treatment plan and good clinical application prospect

Inactive Publication Date: 2019-10-22
PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The clinical manifestations of acute pancreatitis are diverse, with low specificity, and the predictive sensitivity for poor prognosis is lower than 40% (SteinbergWM. 61.), and the pathological basis of systemic inflammatory response syndrome in AP has not yet been clarified

Method used

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  • Biomarkers used for diagnosing pancreatitis and derived from intestinal tract, screening method and application of biomarker
  • Biomarkers used for diagnosing pancreatitis and derived from intestinal tract, screening method and application of biomarker
  • Biomarkers used for diagnosing pancreatitis and derived from intestinal tract, screening method and application of biomarker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Screening of intestinal microbial flora associated with pancreatitis

[0040] 1. Sample collection

[0041] Fresh, middle and late fecal samples were collected from patients with pancreatitis diagnosed in the hospital and normal subjects, and immediately frozen in a -80°C refrigerator. The sample information is shown in Table 1.

[0042] Table 1 Sample information

[0043]

[0044]

[0045] 2. DNA extraction

[0046] Procedure: Stool sample DNA according to MOBIO DNA Isolation Kit 12888-100 instruction manual for extraction.

[0047] The DNA extraction information is shown in Table 2. After the genomic DNA was extracted, the extracted genomic DNA was detected by 1% agarose gel electrophoresis.

[0048] Table 2 DNA extraction information

[0049]

[0050]

[0051] 3. PCR amplification

[0052] According to the designated sequencing region, synthesize specific primers with barcode.

[0053] In order to ensure the accuracy and reliability of ...

Embodiment 2

[0115] The clinical diagnostic value of embodiment 2 intestinal microbial flora

[0116] 9. Model prediction analysis

[0117] Random Forest (Random Forest) belongs to the machine learning algorithm. It is a classifier containing multiple decision trees. Its classification results are judged on different decision trees according to the attributes of each dimension of the detection sample, and all the judgment results are considered comprehensively. Finally, the final classification is given. For the classification problem, the maximum probability is taken, and the regression analysis takes the probability mean. It can efficiently and quickly select the most important species category (biomarker) for sample classification. Software: R (randomForest package), using random forest, setting 500 decision trees, classification level is species, and sorting by importance. For the sorted species, the importance is increased from large to small to build a classification model and calcu...

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Abstract

The invention relates to biomarkers used for diagnosing pancreatitis and derived from the intestinal tract, a screening method and application of the biomarkers. The biomarkers are composed of uncultured_bacterium_g__Eubacterium_hallii_group, Anaerococcus_prevotii_g__Anaerococcus and unclassified_g__Eubacterium_hallii_group. The biomarkers are enriched in intestinal floras. By detecting the abundance degree of the biomarkers, a patient with pancreatitis can be effectively identified, the detection and diagnosis steps of pancreatitis can be simplified, and the diagnosis and treatment efficiencyon pancreatitis are greatly improved.

Description

technical field [0001] The invention relates to the fields of biotechnology, disease diagnosis and biomedicine, in particular to a biomarker for diagnosing pancreatitis derived from the intestinal tract, a screening method and application thereof. Background technique [0002] Acute pancreatitis (AP) is one of the common diseases that cause acute abdominal pain. It is caused by a variety of causes that cause the activation of pancreatic enzymes in the pancreas, causing pancreatic tissue self-digestion, edema, and bleeding, and local inflammation in the pancreas. The main features of the disease, severe disease may occur systemic inflammatory response syndrome (Systemic inflammatory response syndrome, SIRS), accompanied by organ dysfunction (Organ dsfunction, OD). The clinical manifestations of acute pancreatitis are diverse, with low specificity, and the predictive sensitivity for poor prognosis is lower than 40% (SteinbergWM. 61.), and the pathological basis of systemic in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/06G01N33/569C12R1/01
CPCC12Q1/689C12Q2600/106C12Q2600/136G01N33/56911
Inventor 吴东柏小寅张晟瑜李佳宁施文李玥李景南杨爱明
Owner PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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