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A method to increase the amount of cocoon layer

A technology of cocoon layer and silkworm cocoon, applied in the field of active polypeptide synthesis, can solve problems such as lack of research, and achieve the effect of increasing the silk production of silkworms

Active Publication Date: 2020-12-11
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is a lack of research on silkworm neuropeptides regulating cocoon layer volume through energy distribution.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] According to the amino acid sequence pQLTFTSGWGa, an amino acid protected by a 9-fluorenylmethoxycarbonyl (Fmoc) group to the α-amino group is covalently linked to an insoluble solid-phase support (p-alkane) through a p-alkoxybenzyl alcohol arm Oxybenzyl alcohol type resin); use 90% mass fraction of trifluoroacetic acid (TFA) to remove the Fmoc protecting group on the α-amino group, wash the amino acid with 90% mass fraction of the TFA solution-arm-resin, the next A pre-activated α-amino protected amino acid is connected through a coupling reaction; after the reaction is completed, wash with 90% mass fraction of TFA solution, and then repeat the deprotection and coupling reaction until all amino acids are sequenced from the C-terminal (carboxyl terminal) to the N terminal (amino terminal) to obtain the target polypeptide; use hydrogen fluoride (HF) to hydrolyze the ester bond between the peptide chain and the solid phase carrier to obtain the silkworm adipokinin A polype...

Embodiment 2

[0035] According to the amino acid sequence pQLTFTSGWGa, an amino acid protected by a 9-fluorenylmethoxycarbonyl (Fmoc) group to the α-amino group is covalently linked to an insoluble solid-phase support (p-alkane) through a p-alkoxybenzyl alcohol arm Oxybenzyl alcohol type resin); use 90% mass fraction of trifluoroacetic acid (TFA) to remove the Fmoc protecting group on the α-amino group, wash the amino acid with 90% mass fraction of the TFA solution-arm-resin, the next A pre-activated α-amino protected amino acid is connected through a coupling reaction; after the reaction is completed, wash with 90% mass fraction of TFA solution, and then repeat the deprotection and coupling reaction until all amino acids are sequenced from the C-terminal (carboxyl terminal) to the N terminal (amino terminal) to obtain the target polypeptide; use hydrogen fluoride (HF) to hydrolyze the ester bond between the peptide chain and the solid phase carrier to obtain the silkworm adipokinin A polype...

Embodiment 3

[0040] According to the amino acid sequence pQLTFTSGWGa, an amino acid protected by a 9-fluorenylmethoxycarbonyl (Fmoc) group to the α-amino group is covalently linked to an insoluble solid-phase support (p-alkane) through a p-alkoxybenzyl alcohol arm Oxybenzyl alcohol type resin); use 90% mass fraction of trifluoroacetic acid (TFA) to remove the Fmoc protecting group on the α-amino group, wash the amino acid with 90% mass fraction of the TFA solution-arm-resin, the next A pre-activated α-amino protected amino acid is connected through a coupling reaction; after the reaction is completed, wash with 90% mass fraction of TFA solution, and then repeat the deprotection and coupling reaction until all amino acids are sequenced from the C-terminal (carboxyl terminal) to the N terminal (amino terminal) to obtain the target polypeptide; use hydrogen fluoride (HF) to hydrolyze the ester bond between the peptide chain and the solid phase carrier to obtain the silkworm adipokinin A polype...

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Abstract

The invention discloses a method for increasing silkworm cocoon shell weight. The method includes: synthesizing silkworm adipokinetic hormone A polypeptide from terminal C (carboxyl terminal) to terminal N (amino terminal) according to a sequence pQLTFTSGWGa; adopting a luciferase reporter gene method to detect activity of the synthesized silkworm adipokinetic hormone A polypeptide; allowing silkworm adipokinetic hormone A active polypeptide liquid to pass a filtering membrane for degerming, injecting into stomachs of silkworms, and using mulberry leaves to fed till being mature and cocooning,wherein the silkworm cocoon shell weight is increased. The method can increase the silkworm cocoon shell weight without increasing mulberry leaf intake of the silkworms and is realized by regulatingsilkworm energy distribution through newly found active polypeptide, and the method can play a positive role in studying energy distribution mechanism of the silkworms and further increasing silk yield of the silkworms.

Description

technical field [0001] The invention relates to a synthesis of active polypeptides for increasing the cocoon layer of silkworm cocoons, in particular to a method for increasing the cocoon layer of silkworm cocoons. Background technique [0002] The silkworm (Bombyx mori) is the largest economic resource insect on the earth today. It absorbs various nutrients from mulberry leaves and supplies them to various tissues and organs in the silkworm through metabolic and physiological processes for growth, development, and reproduction. Nutrients are supplied to the silk glands to synthesize silk substances to spin silk and form cocoons. Silkworm cocoons are composed of inner silkworm chrysalis and outer cocoon layer. The cocoon layer is made of regular glued cocoon silk. The cocoon silk is drawn out by boiling the cocoon and reeling to produce the raw silk known as the "fiber queen", which has become a traditional precious textile raw material in our country. . For this reason, o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C07K1/06C07K1/04C12Q1/66A01K67/04
CPCA01K67/04C07K7/06C12Q1/66
Inventor 时连根沈张飞屠振力
Owner ZHEJIANG UNIV
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