A method to increase the amount of cocoon layer

Abstract

The invention discloses a method for increasing silkworm cocoon shell weight. The method includes: synthesizing silkworm adipokinetic hormone A polypeptide from terminal C (carboxyl terminal) to terminal N (amino terminal) according to a sequence pQLTFTSGWGa; adopting a luciferase reporter gene method to detect activity of the synthesized silkworm adipokinetic hormone A polypeptide; allowing silkworm adipokinetic hormone A active polypeptide liquid to pass a filtering membrane for degerming, injecting into stomachs of silkworms, and using mulberry leaves to fed till being mature and cocooning,wherein the silkworm cocoon shell weight is increased. The method can increase the silkworm cocoon shell weight without increasing mulberry leaf intake of the silkworms and is realized by regulatingsilkworm energy distribution through newly found active polypeptide, and the method can play a positive role in studying energy distribution mechanism of the silkworms and further increasing silk yield of the silkworms.

Description

technical field

[0001] The invention relates to a synthesis of active polypeptides for increasing the cocoon layer of silkworm cocoons, in particular to a method for increasing the cocoon layer of silkworm cocoons. Background technique

[0002] The silkworm (Bombyx mori) is the largest economic resource insect on the earth today. It absorbs various nutrients from mulberry leaves and supplies them to various tissues and organs in the silkworm through metabolic and physiological processes for growth, development, and reproduction. Nutrients are supplied to the silk glands to synthesize silk substances to spin silk and form cocoons. Silkworm cocoons are composed of inner silkworm chrysalis and outer cocoon layer. The cocoon layer is made of regular glued cocoon silk. The cocoon silk is drawn out by boiling the cocoon and reeling to produce the raw silk known as the "fiber queen", which has become a traditional precious textile raw material in our country. . For this reason, o...