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Magnetic probe for identifying endothelial cells of lymphatic vessel and preparation of probe

A technology of endothelial cells and lymphatic vessels, applied in the field of medicine, can solve the problem of not separating lymphatic vessel endothelial cells

Pending Publication Date: 2019-11-05
GUANGXI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is still no separation of lymphatic endothelial cells with higher purity from mouse intestinal cancer by self-made dual-target magnetic beads

Method used

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  • Magnetic probe for identifying endothelial cells of lymphatic vessel and preparation of probe
  • Magnetic probe for identifying endothelial cells of lymphatic vessel and preparation of probe
  • Magnetic probe for identifying endothelial cells of lymphatic vessel and preparation of probe

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Experimental program
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Embodiment

[0039] Materials and methods

[0040] Cell lines and cell culture conditions

[0041] Colorectal cancer cell line (CT26) was purchased from ATCC cell bank, and CT26 cell line was incubated with DMEM medium containing 10% fetal bovine serum (Fetal Bovine Serum, FBS), 100 U / mL penicillin and 100 U / mL streptomycin at 37 °C, 5% CO 2 cultured in a cell culture incubator.

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Abstract

The invention provides a magnetic probe for identifying endothelial cells of lymphatic vessel and preparation of the probe. The magnetic probe comprises core-shell type Fe3O4@KCTS magnetic nanometer particles and double antibody, modifying the surfaces of the ore-shell type Fe3O4@KCTS magnetic nanometer particles, of lymphatic vessel endothelial cells. The double antibody of lymphatic vessel endothelial cells includes an LYVE-1 antibody or an antibody fragment thereof and a Podoplanin antibody or an antibody fragment thereof. The Fe3O4@KCTS-Double antibody magnetic nanometer particles are usedfor direct incubation and selection of cells to obtain high-purity lymphatic vessel endothelial cells. According to comparison, the cells selected via Fe3O4@KCTS-Double antibody can reach the doubledyeing rate of 88.9% and has higher tube forming and phagocytosis capabilities, and double-modal imaging can be realized for tumour diagnosis.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a magnetic probe for identifying lymphatic endothelial cells and its preparation. Background technique [0002] Accumulating evidence shows that the tumor microenvironment affects tumor biological properties, especially tumor-associated metastasis. However, many of the relevant underlying mechanisms remain poorly understood, especially in lymphatic endothelial cells. To study the role of lymphatic endothelial cells in cancer lymphatic metastasis, a reliable model is needed. Due to the lack of lymphatic vessel-specific markers, especially to distinguish them from vascular endothelium, functional investigations of lymphatic endothelial cells have rarely been reported. In recent years, some specific markers of lymphatic endothelial cells have been identified, such as lymphatic endothelial HA receptor 1 (LYVE-1), Podoplanin, vascular endothelial growth factor receptor 3 (VEGFR-3...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/574
CPCG01N33/54326G01N33/54346G01N33/57496
Inventor 赵永祥钟莉娉黄勇
Owner GUANGXI MEDICAL UNIVERSITY