Alga protein peptide with DPP-IV inhibition function and preparation method of alga protein peptide

A DPP-IV and algal protein technology, which is applied in the field of marine food processing to achieve the effects of good DPP-IV activity inhibition, good functional properties and low ash content

Inactive Publication Date: 2019-11-12
ANHUI GUOTAI BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, there are few reports on the evaluation of DPP-IV inhibitory activity of active peptides derived from algae protein obtained by enzymatic hydrolysis.

Method used

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  • Alga protein peptide with DPP-IV inhibition function and preparation method of alga protein peptide
  • Alga protein peptide with DPP-IV inhibition function and preparation method of alga protein peptide
  • Alga protein peptide with DPP-IV inhibition function and preparation method of alga protein peptide

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preparation example Construction

[0036] About the preparation method of protein peptide of the present invention, comprise the following steps:

[0037] Step1: Prepare Arthrospira protein liquid; select Arthrospira powder and dissolve it in water that meets the sanitary standards for drinking water, wait for it to completely dissolve, and use a high-speed shearing machine to process it for 2-3 minutes to obtain Arthrospira protein liquid.

[0038] Step2: protein modification; the Arthrospira protein liquid obtained in step1 is treated with an ultrasonic generator at a frequency of 60-90kHz for 20-30 minutes, and then treated with an ultra-high pressure microjet with a pressure of 200-260Mpa 1-3 Second, change the organizational structure of the Arthrospira protein to obtain the allosteric Arthrospira protein liquid.

[0039] Step3: step-by-step enzymatic hydrolysis; adjust the protein content of the Arthrospira protein solution obtained in step2 to 7-10%, and then add compound protease and flavor protease for...

Embodiment 1

[0054] Step1: Prepare Arthrospira protein liquid; select Arthrospira powder and dissolve it in water that meets the sanitary standards for drinking water, wait for it to completely dissolve, and use a high-speed shearing machine to process it for 2 minutes to obtain Arthrospira protein liquid.

[0055] Step2: protein modification; the Arthrospira protein liquid obtained in step1 was treated with an ultrasonic generator at a frequency of 60kHz for 20 minutes, and then treated once with an ultra-high pressure microjet with a pressure of 200Mpa to change the Arthrospira protein The organizational structure of the modified Arthrospira protein solution was obtained.

[0056] Step3: step-by-step enzymatic hydrolysis; adjust the protein content in the Arthrospira protein solution obtained in step2 to 7%, and then add compound protease and flavor protease in sequence for enzymolysis to obtain an enzymolysis solution.

[0057] Step4: secondary ultrafiltration; heat the enzymolysis solu...

Embodiment 2

[0071] Step1: Prepare Arthrospira protein liquid; select 50 g of Arthrospira powder and dissolve it in water that meets the sanitary standards for drinking water. After it is completely dissolved, use a high-speed shearing machine to process for 3 minutes to obtain Arthrospira protein liquid.

[0072] Step2: protein modification; the Arthrospira protein liquid obtained in step1 is treated with an ultrasonic generator at a frequency of 90kHz for 30 minutes, and then treated 3 times with an ultra-high pressure microjet with a pressure of 260Mpa to change its Arthrospira protein The organizational structure of the modified Arthrospira protein solution was obtained.

[0073] Step3: step-by-step enzymatic hydrolysis; adjust the protein content in the Arthrospira protein solution obtained in step2 to 10%, and then add compound protease and flavor protease in sequence for enzymolysis to obtain an enzymolysis solution.

[0074] Step4: secondary ultrafiltration; heat the enzymolysis so...

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Abstract

The invention relates to alga protein peptide with a DPP-IV inhibition function and a preparation method of the alga protein peptide. The alga protein peptide comprises components as follows: polypeptide with amino acid sequences of HGERVVI, TPDVSIS and YPLIDVK, and the content of the polypeptide is 52%-58%. The preparation method of the alga protein peptide with the DPP-IV inhibition function comprises the steps as follows: Step 1, preparation of a arthrospira protein solution; Step 2, protein allosterism; Step 3, stepped enzymolysis; Step 4, secondary ultrafiltration; Step 5, repeated elution; and Step 6, ingredient measurement.

Description

technical field [0001] The invention relates to the technical field of marine food processing, in particular to an algal protein peptide with DPP-IV inhibitory function and a preparation method thereof. Background technique [0002] Hypoglycemic functional factors refer to biologically active components that can reduce blood sugar concentration and improve symptoms of diabetic patients. At present, there are many natural product-type hypoglycemic factors, mineral-type hypoglycemic factors, and vitamin-type hypoglycemic factors with different mechanisms of action. Natural product hypoglycemic factors can be divided into flavonoids, active polysaccharides, alkaloids, saponins, terpenes, conjugated linoleic acid, polypeptides, etc. according to their chemical structures. Since the artificial synthesis of insulin, various oral or injectable drugs such as sulfonylureas, biguanides, insulin sensitizers, and sugar inhibitors have come out one after another. However, chemical drugs...

Claims

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Application Information

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IPC IPC(8): C07K7/06C07K1/14C07K1/36C07K1/34C07K1/20C07K1/16C12P21/06
CPCC07K7/06C12P21/06
Inventor 张恒罗永康刘怀高于海波
Owner ANHUI GUOTAI BIOTECHNOLOGY CO LTD
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