A method for the determination of bittering agents naringin and denatonium benzoate in children's finger painting ink pads by ultra-high performance liquid chromatography

Abstract

The invention discloses a method for determining the bittering agent naringin and denatonium benzoate in children's finger painting ink pads by ultra-high performance liquid chromatography. 2 PO 4 (30:70, v / v) was used as solvent, and two kinds of bittering agents in children's finger painting ink pad samples were extracted by ultrasonic extraction, quantified by external standard method, and analyzed by UPLC. The linear range of naringin and denatonium benzoate is 0.1~5mg / L (R 2 ≥0.99), the detection limits (S / N>3) were 0.75 and 1.15mg / kg, respectively. The spiked recoveries of naringin and denatonium benzoate in children's finger painting ink pads at three concentration levels ranged from 86.7% to 108.4%; the relative standard deviation (RSD, n=6) was less than 5.0%.

Description

technical field [0001] The invention relates to a detection method, in particular to a method for detecting bittering agents naringin and denatonium benzoate in children's finger painting ink pads by ultra-high performance liquid chromatography. Background technique [0002] Bitter agent (Aversive agent), also known as aversive agent and anti-feeding agent, is a kind of product added to make its taste or smell bitter to prevent people, especially children, from inhaling or ingesting toxic and harmful substances, rodents and birds. Chemical agents widely used to destroy facilities. Denatonium benzoate, also known as Bitrex, is the most bitter compound known so far and is a typical representative of bittering agents. Bitter essence has stable chemical properties, low price, is easy to obtain, and is extremely soluble in water. In addition, its mammalian lethal dose (Median lethal dose, LD50) is 841mg / kg body weight, and only a small amount of addition (0.00001% ) can have an...

AI Technical Summary

Problems solved by technology

[0005] The matrix of children’s finger painting ink pads is complex. In order to avoid interference, it is necessary to add an extraction solvent with a high dilution factor for extraction. The standard recommended concentration of denatonium benzoate is 4 mg / kg, which is a challenge for the sensitivity of the instrument and method. The sensitivity of the instrument and assay method of above-mentioned denatonium benzoate can not meet the requirement

[0006] Children's finger painting inkpad is one of the finger painting pigments. Children use their fingers to dip the paint on the inkpad for creation. Children's consciousness is weak. If children's inkpad products do not add bittering agents or the amount of addition does not meet the standard requirements, it is easy to lick children's body and mind. serious health hazard

Ultra-high performance liquid chromatography has the advantages of fast analysis speed and high separation efficiency. At present, there is no report on the analysis of bittering agents naringin and denatonium benzoate in children's finger painting ink pads by ultra-high performance liquid chromatography.

Images