Micro total analysis chip and in-situ reaction method
A technology of micro-total analysis and in-situ reaction, which is applied in the direction of material analysis, material excitation analysis, and material analysis through optical means, which can solve the problems of loss of preset samples and deviation of analysis results, etc., to simplify complexity and improve reliability. controlling effect
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Embodiment 1
[0053] This embodiment is used to illustrate a micro-total analysis chip pre-installed with a reagent solid reagent and an in-situ reaction method using the micro-total analysis chip.
[0054] Such as figure 1 As shown in (b), the KSCN solid is packaged with a soluble film by hot pressing to obtain a capsule, and then placed in the reaction pool of the micro total analysis chip. The soluble film used is a polyvinyl alcohol film with a thickness of 0.020 mm and a stable period of 5 s. The solution to be tested is introduced through the injection port and the flow channel of the micro total analysis chip. The soluble film remained intact for 5 s before the start of solution introduction, during which the solution was introduced and filled the reaction cell. After 5 s, the soluble film encapsulating the KSCN solid dissolved, and the solid sample in the film was released, and the Fe in the solution 3+ The solution was reacted to form a reddish-brown solution, which proved that ...
Embodiment 2
[0056] This embodiment is used to illustrate a micro-total analysis chip pre-installed with capsules packaged with different reagents and an in-situ reaction method using the micro-total analysis chip.
[0057] Such as figure 1 As shown in (c), the powdered yellow dye is encapsulated with a polyvinyl alcohol soluble film with a thickness of 0.023mm to obtain capsule a (stable period is 7s), and the powdered blue dye is encapsulated with a polyethylene film with a thickness of 0.046mm Capsule b was obtained by encapsulation with an alcohol-soluble film (the stability period was 3 minutes), and then the capsules a and b were simultaneously preset in the same reaction pool of the micro-total analysis chip. Introduce deionized water through the flow channel of the micro total analysis chip. During the 7 second period during the introduction of deionized water, the encapsulation film remained intact. After 7 seconds, the soluble film in capsule a began to dissolve, and the yellow...
Embodiment 3
[0059] This embodiment is used to illustrate a micro-total analysis chip pre-installed with capsules that simultaneously encapsulate reagents and capsules, and an in-situ reaction method using the micro-total analysis chip.
[0060] Such as figure 1 As shown in (b), the periodic acid solid is encapsulated with a polyvinyl alcohol soluble film with a thickness of 0.046mm to obtain capsule b (the stability period is 3min), and then the NaOH solid and capsule b are jointly used with a polyethylene film with a thickness of 0.023mm. Capsule a (with a stable period of 7s) was obtained by encapsulation with an alcohol-soluble film, and then the capsule a was preset in the reaction pool of the micro-total analysis chip. The phenolphthalein solution was introduced through the flow channel of the micro total analysis chip. The encapsulation membrane remains in place for a period of 7 seconds during liquid sample introduction. After 7 seconds, the soluble film in capsule a begins to di...
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Abstract
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