Application of grass fruit extract

A technology of extracts and tsaoko, applied in the application field of tsaoko extract, can solve the problems of long treatment course, poor curative effect, obvious side effects of drugs, etc.

Active Publication Date: 2021-10-22
CAPITALBIO CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the treatment plan for multidrug-resistant tuberculosis has limitations, the side effects of the drug are obvious, the curative effect is not good, the course of treatment is long, and the price is expensive.
At present, there is no report on the use of Tsaoguo extract for the preparation of drugs for the treatment of multidrug-resistant tuberculosis

Method used

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  • Application of grass fruit extract
  • Application of grass fruit extract
  • Application of grass fruit extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The preparation of alcohol extract under embodiment 1, different conditions

[0035] Take 10g of the mature fruit of Cao Guo, add 200mL of 80% ethanol (volume percentage, the remaining 20% ​​is water) and use a Soxhlet extractor to heat to boiling and reflux for extraction for 4h, and the extract is filtered and concentrated at 45°C to obtain an extract. The paste was frozen at -80°C, and then vacuum freeze-dried at -60 to -40°C for 24 hours to obtain a freeze-dried product, namely the tsokurol extract S1.

[0036]Take 10g of mature fruit of Cao Guo, add 150mL of 90% ethanol (volume percentage, the remaining 10% is water) and use a Soxhlet extractor to heat to boiling and reflux for extraction for 3h, and the extract is filtered and concentrated at 45°C to obtain an extract. The paste was frozen at -80°C, and then vacuum freeze-dried at -60 to -40°C for 24 hours to obtain a freeze-dried product, namely the tsokurol extract S2.

[0037] Take 10g of the mature fruit of C...

Embodiment 2

[0040] Embodiment 2, the preparation of extract

[0041] Take 10g of mature fruit of Cao Guo, add 150mL of 90% ethanol (volume percentage, the remaining 10% is water) and use a Soxhlet extractor to heat to boiling and reflux for extraction for 3h, and the extract is filtered and concentrated at 45°C to obtain an extract. The paste was frozen at -80°C, and then vacuum freeze-dried at -60 to -40°C for 24 hours to obtain a freeze-dried product, namely the turmeric alcohol extract.

[0042] Take 10g of the mature fruit of Cao Guo, add 150mL of deionized water and use a Soxhlet extractor to heat it to boiling and reflux for 3 hours, filter the extract and concentrate it at 45°C to obtain an extract, freeze the extract at -80°C, and then store it at -60~- Vacuum freeze-drying at 40° C. for 24 hours to obtain a freeze-dried product, namely the Tsaoko water extract.

[0043] Take 10g of the mature fruit of the southern crane locust, add 150mL of 90% ethanol (volume percentage, the re...

Embodiment 3

[0044] Embodiment 3, alcohol extract anti-tuberculosis experiment

[0045] 1. Materials and methods

[0046] (1) Test drug: the tsokurol extract prepared according to the method of Example 2 of the present invention was prepared into a mother solution with a concentration of 50 mg / mL with dimethyl sulfoxide (DMSO). The grass fruit water extract prepared by the method of Example 2 of the present invention was prepared into a mother solution with a concentration of 50 mg / mL with deionized water.

[0047] (2) Test tuberculosis strains: standard strain H37Rv (not drug-resistant); clinical strains 00419, 221090, 221114, 221136, 221173, 222155 (not drug-resistant, provided by the Chinese Center for Disease Control and Prevention, Center for Tuberculosis Prevention and Control); multi-drug resistant Strains MDR-A07 (resistant to rifampicin + isoniazid + ethambutol + rifabutin + levofloxacin + moxifloxacin + p-aminosalicylic acid + ethionamide), MDR-A08 (resistant to rifampicin MDR-...

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Abstract

The invention discloses the application of tsaoko extract. The invention provides the application of the herbal alcohol extract in the preparation of drug-resistant tuberculosis drugs, and the application in the preparation of drugs for inhibiting drug-resistant Mycobacterium tuberculosis. The invention adopts a Soxhlet extraction method, uses ethanol with a concentration of 80%-100% as a solvent for the grass fruit, heats and refluxes extraction, filters and concentrates, and freezes and dries to obtain the grass fruit alcohol extract. It has been proved by experiments that the alcohol extract of tsao berry has a strong anti-tuberculosis effect on Mycobacterium tuberculosis, especially multi-drug resistant Mycobacterium tuberculosis, and the cytotoxicity evaluation results of the alcohol extract of tsao berry have confirmed that its concentration is not more than 250 μg / mL No obvious cytotoxicity to human embryonic lung fibroblast MRC‑5. Therefore, the present invention has great significance for the development of anti-tuberculosis drugs, especially anti-drug-resistant tuberculosis drugs.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of grass fruit extract. Background technique [0002] Tuberculosis is a chronic infectious disease caused by Mycobacterium tuberculosis infection, which is mainly transmitted through the respiratory tract. It is one of the diseases with the longest history and serious consequences that endanger human life and health. China is a big tuberculosis country. With the application of antibiotics, drug-resistant tuberculosis has gradually become a difficult problem in the prevention and control of tuberculosis. At present, the situation of tuberculosis prevention and control is still very grim. According to the 2018 global tuberculosis report provided by the World Health Organization, there are about 10 million new tuberculosis patients in the world, and about 890,000 people in China. It is estimated that there were 560,000 drug-resistant tuberculosis cases in the worl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/9064A61K45/06A61P11/00A61P31/06A61K131/00
CPCA61K36/9064A61K45/06A61K2236/333A61K2236/51A61P11/00A61P31/06
Inventor 郭弘妍杨春花余金金邢婉丽程京
Owner CAPITALBIO CORP
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