Preparation method and application of chitosan immunoaffinity adsorbent for mycotoxins

A technology of mycotoxins and immunophiles, which is applied in chemical instruments and methods, selective adsorption, alkali metal oxides/hydroxides, etc., can solve problems such as limited applications, and achieve wide pH stability, uniform particle size, and source rich effect

Inactive Publication Date: 2019-12-06
JIANGSU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The currently prepared chitosan microspheres as carrier chromatographic fillers still have some shortcomings in terms of microsphere size uniformity and stability under acidic conditions, which limit its application.

Method used

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  • Preparation method and application of chitosan immunoaffinity adsorbent for mycotoxins
  • Preparation method and application of chitosan immunoaffinity adsorbent for mycotoxins
  • Preparation method and application of chitosan immunoaffinity adsorbent for mycotoxins

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Embodiment 1

[0027] Embodiment 1 Preparation of a mycotoxin chitosan microsphere immunoaffinity adsorbent

[0028] (1) Preparation of uniform particle size, pH stable chitosan microspheres:

[0029] a. Get 0.5g chitosan, dissolve in 50mL 2% acetic acid solution and be made into 1% chitosan solution;

[0030] b. Prepare 300mL of liquid paraffin and petroleum ether (v:v, 7:5) mixed oil phase (containing 4% span 80);

[0031] c. Pour the chitosan solution into the feeding tank of the membrane emulsifier, adjust the nitrogen pressure to 0.02MPa, and the magnetic stirring speed of the oil phase to 500rpm, so that the chitosan solution is pressed into the oil phase through the SPG membrane under nitrogen pressure ;

[0032] d. Add 7.5 mL of cross-linking agent for cross-linking reaction for 1 hour, then add 5 mL of 1M sodium hydroxide solution, and continue cross-linking for 2 hours;

[0033] e. After cross-linking, wash with petroleum ether, ethanol, and water until neutral, and wash with 5M...

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Abstract

The invention discloses a preparation method and application of a chitosan immunoaffinity adsorbent for mycotoxins, and belongs to the technical field of development and application of natural polysaccharides and preparation of functional materials. The preparation method comprises the steps: preparing a chitosan solution and an oil phase which is composed of liquid paraffin, an emulsifier span 80and petroleum ether, pouring the chitosan solution into a material inlet tank of a membrane emulsification machine, adjusting the nitrogen pressure and the rotation speed of magnetic stirring of theoil phase so as to make the chitocan solution pass through SPG membrane pores slowly and be pressed into the oil phase under the action of nitrogen pressure, adding a cross-linking agent after completion of emulsification, performing stirring for cross-linking, then performing washing by using petroleum ether, performing washing by using ethanol and water sequentially, performing reduction, performing water washing again so as to obtain chitosan microspheres, performing coupling on the chitosan microspheres and mycotoxin antibodies, performing washing by using distilled water and PBS so as toobtain the chitosan microsphere immunoaffinity adsorbent finally. The prepared chitosan microsphere immunoaffinity adsorbent has uniform particle size and stability within a wide pH range, and can beapplied to purifying separation and determination of the mycotoxins in food samples.

Description

technical field [0001] The invention relates to a preparation method and application of a mycotoxin chitosan immunoaffinity adsorbent, and belongs to the technical fields of development and application of natural polysaccharides and preparation of functional materials. Background technique [0002] Mycotoxins widely contaminate food, oilseeds, and food and other plant-derived products, which pose a huge threat to human health, and have carcinogenic, teratogenic, and mutagenic effects on animals and humans. Among the more serious mycotoxins, aflatoxins, zearalenone toxins, deoxynivalenol and fumonisins are the most representative. The World Health Organization (WHO) also listed the contamination of agricultural products by mycotoxins as an important source of foodborne diseases in 2002. At present, the detection of mycotoxin residues in agricultural products is mainly based on instrumental analysis methods such as HPLC and HPLC-MS. Due to the complex matrix and low mycotoxi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/24B01J20/28B01J20/30B01D15/08
CPCB01D15/08B01J20/24B01J20/28021
Inventor 王云牛瑞张成罗世龙王姝文杨雅梅
Owner JIANGSU UNIV
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