Preparation method and application of chitosan immunoaffinity adsorbent for mycotoxins

Abstract

The invention discloses a preparation method and application of a chitosan immunoaffinity adsorbent for mycotoxins, and belongs to the technical field of development and application of natural polysaccharides and preparation of functional materials. The preparation method comprises the steps: preparing a chitosan solution and an oil phase which is composed of liquid paraffin, an emulsifier span 80and petroleum ether, pouring the chitosan solution into a material inlet tank of a membrane emulsification machine, adjusting the nitrogen pressure and the rotation speed of magnetic stirring of theoil phase so as to make the chitocan solution pass through SPG membrane pores slowly and be pressed into the oil phase under the action of nitrogen pressure, adding a cross-linking agent after completion of emulsification, performing stirring for cross-linking, then performing washing by using petroleum ether, performing washing by using ethanol and water sequentially, performing reduction, performing water washing again so as to obtain chitosan microspheres, performing coupling on the chitosan microspheres and mycotoxin antibodies, performing washing by using distilled water and PBS so as toobtain the chitosan microsphere immunoaffinity adsorbent finally. The prepared chitosan microsphere immunoaffinity adsorbent has uniform particle size and stability within a wide pH range, and can beapplied to purifying separation and determination of the mycotoxins in food samples.

Description

technical field [0001] The invention relates to a preparation method and application of a mycotoxin chitosan immunoaffinity adsorbent, and belongs to the technical fields of development and application of natural polysaccharides and preparation of functional materials. Background technique [0002] Mycotoxins widely contaminate food, oilseeds, and food and other plant-derived products, which pose a huge threat to human health, and have carcinogenic, teratogenic, and mutagenic effects on animals and humans. Among the more serious mycotoxins, aflatoxins, zearalenone toxins, deoxynivalenol and fumonisins are the most representative. The World Health Organization (WHO) also listed the contamination of agricultural products by mycotoxins as an important source of foodborne diseases in 2002. At present, the detection of mycotoxin residues in agricultural products is mainly based on instrumental analysis methods such as HPLC and HPLC-MS. Due to the complex matrix and low mycotoxi...

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Problems solved by technology

The currently prepared chitosan microspheres as carrier chromatographic fillers still have some shortcomings in terms of microsphere size uniformity and stability under acidic conditions, which limit its application.

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