A gene, protein, carrier, recombinant genetically engineered bacterium and application thereof for accelerating fruit ripening

A mature and genetic technology, applied in the field of agricultural biology, can solve problems such as patent publications that have not yet been found, and achieve good application prospects, good fruit commerciality, and the effect of accelerating fruit ripening

Active Publication Date: 2021-11-30
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Through the search, no patent publications related to the patent application of the present invention have been found

Method used

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  • A gene, protein, carrier, recombinant genetically engineered bacterium and application thereof for accelerating fruit ripening
  • A gene, protein, carrier, recombinant genetically engineered bacterium and application thereof for accelerating fruit ripening

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Embodiment Construction

[0028] The embodiments of the present invention will be described in detail below. It should be noted that the embodiments are illustrative, not restrictive, and cannot limit the protection scope of the present invention.

[0029] The raw materials used in the present invention, unless otherwise specified, are conventional commercially available products; the methods used in the present invention, unless otherwise specified, are conventional methods in the art.

[0030] A gene for accelerating fruit ripening, said gene is: the coding region is a DNA molecule shown in SEQ ID NO.1;

[0031] Alternatively: the coding region is a DNA molecule having 90% or more homology with the DNA molecule shown in SEQ ID NO.1.

[0032] The purpose of the present invention is to provide a gene (FRA gene, Fruit ripeningaccelerator) that accelerates fruit ripening, due to the particularity of the nucleotide sequence, any variant of the polynucleotide shown in SEQ ID NO.1, as long as it is compatib...

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Abstract

The present invention relates to a gene for accelerating fruit ripening, said gene is: the coding region is a DNA molecule shown in SEQ ID NO.1; or: the coding region is 90% identical to the DNA molecule shown in SEQ ID NO.1 and above homologous DNA molecules. For the first time, the present invention excavates a gene (FRA gene) that promotes early fruit ripening outside the ethylene biosynthesis pathway, and constructs a carrier, realizing the innovative research and development of new germplasm of early-maturing fruit by using the gene transgenic method. Expression of FRA gene can accelerate fruit ripening.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, in particular to a gene (FRA gene) for accelerating fruit ripening, a protein, a carrier, a recombinant genetically engineered bacterium and applications thereof. Background technique [0002] The phenomenon of fruit ripening is mainly controlled by a series of physiological and biochemical reactions triggered by ethylene. During the fruit ripening process, the respiration is strengthened, the starch is hydrolyzed into soluble sugars, and the organic acid is decomposed, which increases the sweetness of the fruit and reduces the sour taste; the hydrolysis of pectin makes the cell wall thinner, the fruit firmness decreases and softens, and the chlorophyll pigments degrade , Anthocyanins and carotenoid pigments are synthesized in large quantities, so that the color of the peel changes from green to red or yellow. EFE (ethylene forming enzyme) is a key enzyme in ethylene biosynthesis. The ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/84A01H5/00A01H6/82
CPCC07K14/415C12N15/8261
Inventor 冯涛李爱
Owner TIANJIN AGRICULTURE COLLEGE
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