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Lactobacillus paracasei for oil degradation and application thereof

A technology of Lactobacillus and para-cheese, applied in the field of lactic acid bacteria, can solve the problems such as the taste and flavor of high-fat food that are difficult to resist

Active Publication Date: 2020-11-13
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the ways to reduce fat intake are mainly to change food cooking methods, reduce intake or use fat substitutes, etc. Although these methods can reduce fat intake to a certain extent, it is still difficult for people to resist the temptation of high-fat foods taste and flavor

Method used

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  • Lactobacillus paracasei for oil degradation and application thereof
  • Lactobacillus paracasei for oil degradation and application thereof
  • Lactobacillus paracasei for oil degradation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] 1. Strain screening

[0074] (1) Domestication of strains

[0075] Under aseptic conditions, use a pipette gun to draw appropriate amount of samples of five kinds of distiller's grains, which are marked as "S1, S2, S3, S4, S5" respectively, and inoculate these five kinds of distiller's grains samples into MRS and M17 medium, respectively. They were cultured in a constant temperature incubator at 37°C and 42°C, with a total of 20 parallel samples, and then the initially cultured strains were streaked and domesticated.

[0076] After 48 hours of acclimatization culture in MRS and M17 medium, the strain culture results in distiller's grains were as follows: figure 1 , as shown in Table 2, the colonies in only 16 petri dishes are more in line with the colony morphological characteristics of lactic acid bacteria in appearance, and the primary screening culture is further carried out.

[0077] Table 2 Strain acclimation results

[0078]

[0079]

[0080] (2) Prelimin...

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PUM

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Abstract

The invention discloses an oil-degrading Lactobacillus paracasei F3, and the preservation number is CGMCC No.18355. Lactobacillus paracasei F3 can degrade blend oil, peanut oil and sesame oil, and the degradation effect on sesame oil is the best, with a degradation rate of 39.38%. The strain can detect obvious lipase activity, and the enzyme activity is as high as 11.08U / mL. At the same time, Lactobacillus paracasei F3 can degrade a variety of fatty acids in sesame oil, especially fatty acids such as linoleic acid, oleic acid, elaidic acid, palmitic acid, and linolenic acid, and the total degradation rate of fatty acids in sesame oil can reach 6.71mg / mL. Lactobacillus paracasei F3 exhibited excellent acid and bile salt resistance in the tolerance test of artificial gastric juice, artificial intestinal juice and bile salt, and can tolerate human gastric juice and intestinal juice, and can be used as a probiotic with excellent performance to survive in the intestinal tract and play a role. The Lactobacillus paracasei F3 of the present invention can be used as a food additive or directly made into food, health products and the like.

Description

technical field [0001] The invention belongs to the technical field of lactic acid bacteria, and relates to a strain of Lactobacillus paracasei which degrades oil and its application. Background technique [0002] Dietary fat is an important source of energy for the human body and plays an important role in maintaining the energy balance of the human body. With the development of the economy, adequate food supply and other factors, dietary fat intake has increased significantly. Excessive dietary fat intake can promote the occurrence of chronic diseases such as obesity, coronary heart disease, diabetes and tumors. At present, the ways to reduce fat intake are mainly to change food cooking methods, reduce intake or use fat substitutes, etc. Although these methods can reduce fat intake to a certain extent, it is still difficult for people to resist the temptation of high-fat foods taste and flavor. [0003] After the fat is ingested, it mainly depends on the pancreatic lipas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23C19/06A23L13/40A23L33/135C12R1/225
CPCC12N1/20A23C19/062A23L13/45A23L33/135A23V2002/00C12R2001/225C12N1/205A23V2400/165A23V2200/3204
Inventor 关成冉陶志强王丽顾瑞霞陈大卫张臣臣黄玉军陈霞马文龙
Owner YANGZHOU UNIV
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