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Lactobacillus helveticus, direct vat set starter for high-yield GABA and application of direct vat set starter.

A technology of direct input starter and lactobacillus, applied in the field of microorganisms, can solve the problems of poor fermentation performance and restrictions on the development and application of dairy products rich in γ-aminobutyric acid, and achieve high safety, excellent acid resistance and bile salt resistance Performance, Effect of High GABA Content

Active Publication Date: 2021-11-26
HANGZHOU WAHAHA TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, even though some lactic acid bacteria currently exist can metabolize GABA, they are not included in the "List of Strains Applicable to Food", or some strains have poor fermentation performance in the fermentation system containing dairy products such as milk or reconstituted milk. Development and application of dairy products rich in γ-aminobutyric acid

Method used

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  • Lactobacillus helveticus, direct vat set starter for high-yield GABA and application of direct vat set starter.
  • Lactobacillus helveticus, direct vat set starter for high-yield GABA and application of direct vat set starter.
  • Lactobacillus helveticus, direct vat set starter for high-yield GABA and application of direct vat set starter.

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Effect test

Embodiment 1

[0030] Example 1: Screening and identification of high-yield gamma-aminobutyric acid strains

[0031] 1 Screening of high-yielding gamma-aminobutyric acid strains

[0032] (1) Preparation of culture medium

[0033] MRS liquid medium: glucose 20g, yeast extract 5g, beef extract 10g, peptone 10g, dipotassium hydrogen phosphate 2g, sodium acetate 5g, diammonium hydrogen citrate 2g, magnesium sulfate heptahydrate 0.58g, manganese sulfate monohydrate 0.25g, Tween 80 1mL, distilled water 1000mL, sterilize at 115°C for 20min.

[0034] MRS solid medium: add 15g / L agar on the basis of MRS liquid medium.

[0035] MRS fermentation medium: Add 10 g / L inducer (sodium glutamate) on the basis of MRS liquid medium.

[0036] (2) Screening of lactic acid bacteria

[0037] Take 1 mL of dairy products such as yogurt and milk lumps sampled in Xinjiang and add them to 9 mL of sterile saline to make 10 -1 Concentration of the sample solution, after shaking and mixing, gradually dilute to the ap...

Embodiment 2

[0074] Embodiment 2: Acid-resistant and bile-salt-resistant ability of bacterial strains

[0075] Acid tolerance of the strain

[0076] After the 3878 and 3881 strains were subcultured twice, the bacterial liquid at the end of logarithmic growth was taken, centrifuged at 4000r / min for 10min, and the supernatant was removed to obtain the bacterial sludge. Add MRS or M17 solution with the same volume of pH 2.5 as the culture solution, pipette and mix well, incubate at 37°C, and measure the changes in the number of bacteria at 0 point and after incubation for 1h, 2h, and 3h by the dilution coating counting method, and set three parallel. The number of viable bacteria measured at different times is shown in Table 2.

[0077] Table 2

[0078]

[0079]

[0080] The results of the acid resistance test showed that pH 2.5 had no significant effect on the viability of the two strains at 2 hours. At 3 hours, the number of viable bacteria of the 3878 strain decreased by 0.7 order...

Embodiment 3

[0086] Embodiment 3: the preparation of the direct throw type starter of high yield GABA

[0087] 1 Preparation of 3878 and 3881 strain freeze-dried bacterial powder

[0088] Lactobacillus helveticus 3878 strain preserved in glycerol tubes was inoculated in its optimized medium at an inoculation amount of 1%, and cultured in a 37°C incubator for 24 hours. After 3 generations of activation, it was inoculated in a 10L fermenter for high-density anaerobic culture. Cultivate at 37°C for 15 hours to obtain a fermentation broth. Centrifuge at 8000r / min at 4°C for 15min, discard the supernatant, collect the bacterial cell pellet, rinse the bacterial cell once with sterile phosphate buffer (pH 7.0), and obtain the bacterial sludge of the 3878 strain.

[0089] Streptococcus thermophilus 3881 strain stored in glycerol tubes was inoculated in its optimized medium at an inoculation amount of 1%, cultured in a 43°C incubator for 24 hours, and after 3 generations of activation, inoculated ...

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Abstract

The invention relates to the technical field of microorganisms, and discloses lactobacillus helveticus, a direct vat set starter for high-yield GABA and application of the direct vat set starter. The direct vat set starter comprises lactobacillus helveticus 3878 and streptococcus thermophilus 3881 which are both preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation numbers of the lactobacillus helveticus 3878 and the streptococcus thermophilus 3881 are CGMCC No.21812 and CGMCC No.21811 respectively. After the two strains are compounded, the two strains can play a synergistic role, and compared with a single strain, the strain has higher gamma-aminobutyric acid production efficiency.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a Lactobacillus helveticus, a high-yield GABA direct-throwing starter and an application thereof. Background technique [0002] γ-aminobutyric acid (GABA for short), also known as aminobutyric acid, gamma aminobutyric acid, etc., is a non-protein amino acid widely present in animals, plants and microorganisms. Important inhibitory neurotransmitter. It participates in a variety of metabolic activities in the body and has important physiological functions, such as calming nerves, improving sleep, beautifying skin, lowering blood pressure, anti-epilepsy, anti-depression, delaying brain aging, supplementing human inhibitory neurotransmitters, and promoting kidney function. Improve and inhibit fatty liver and obesity. Because of its excellent physical and chemical properties, GABA is currently widely used in food, medicine and other fields, and has always been a research hotspot at...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23C9/123C12R1/225C12R1/46
CPCC12N1/20A23C9/1234A23C9/1238A23V2400/147A23V2400/249
Inventor 周晴晴陈苏李理高侃陈作国郑志瑶林国栋葛红娟李言郡余腾斐朱珺俞赟霞
Owner HANGZHOU WAHAHA TECH
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