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Streptococcus thermophilus, sleep-aiding fermented milk base material rich in GABA, lactic acid bacteria beverage and preparation method

A technology of Streptococcus thermophilus and lactic acid bacteria beverages, which is applied in the fields of sleep aid fermented milk base material, lactic acid bacteria beverages and preparation, can solve the problems of limited application, high cost, and long fermentation time, and achieve high safety, improved efficiency, and taste smooth effect

Pending Publication Date: 2021-12-21
HANGZHOU WAHAHA TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the GABA-containing lactic acid bacteria beverages currently on the market and in the prior art are exogenously added with GABA (such as patent CN101467556), the fermentation time is long (generally more than 3 days), and the cost is high, which limits its application.

Method used

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  • Streptococcus thermophilus, sleep-aiding fermented milk base material rich in GABA, lactic acid bacteria beverage and preparation method
  • Streptococcus thermophilus, sleep-aiding fermented milk base material rich in GABA, lactic acid bacteria beverage and preparation method
  • Streptococcus thermophilus, sleep-aiding fermented milk base material rich in GABA, lactic acid bacteria beverage and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1: Screening and identification of high-yield gamma-aminobutyric acid strains

[0056] 1 Screening of high-yielding gamma-aminobutyric acid strains

[0057] (1) Preparation of culture medium

[0058] MRS liquid medium: glucose 20g, yeast extract 5g, beef extract 10g, peptone 10g, dipotassium hydrogen phosphate 2g, sodium acetate 5g, diammonium hydrogen citrate 2g, magnesium sulfate heptahydrate 0.58g, manganese sulfate monohydrate 0.25g, Tween 80 1mL, distilled water 1000mL, sterilize at 115°C for 20min.

[0059] MRS solid medium: add 15g / L agar on the basis of MRS liquid medium.

[0060] MRS fermentation medium: Add 10 g / L inducer (sodium glutamate) on the basis of MRS liquid medium.

[0061] (2) Screening of lactic acid bacteria

[0062] Take 1 mL of dairy products such as yogurt and milk lumps sampled in Xinjiang and add them to 9 mL of sterile saline to make 10 -1 Concentration of the sample solution, after shaking and mixing, gradually dilute to the ap...

Embodiment 2

[0100] Embodiment 2: Acid-resistant and bile-salt-resistant ability of bacterial strains

[0101] Acid tolerance of the strain

[0102] After the 3878 and 3881 strains were subcultured twice, the bacterial liquid at the end of logarithmic growth was taken, centrifuged at 4000r / min for 10min, and the supernatant was removed to obtain the bacterial sludge. Add MRS or M17 solution with the same volume of pH 2.5 as the culture solution, pipette and mix well, incubate at 37°C, and measure the changes in the number of bacteria at 0 point and after incubation for 1h, 2h, and 3h by the dilution coating counting method, and set three parallel. The number of viable bacteria measured at different times is shown in Table 2.

[0103] Table 2

[0104]

[0105] The results of the acid resistance test showed that pH 2.5 had no significant effect on the viability of the two strains at 2 hours. At 3 hours, the number of viable bacteria of the 3878 strain decreased by 0.7 orders of magnitu...

Embodiment 3

[0111] Embodiment 3: the preparation of direct-throwing type starter

[0112] 1 Preparation of 3878 and 3881 strain freeze-dried bacterial powder

[0113] Lactobacillus helveticus 3878 strain preserved in glycerol tubes was inoculated in its optimized medium at an inoculation amount of 1%, and cultured in a 37°C incubator for 24 hours. After 3 generations of activation, it was inoculated in a 10L fermenter for high-density anaerobic culture. Cultivate at 37°C for 15 hours to obtain a fermentation broth. Centrifuge at 8000r / min at 4°C for 15min, discard the supernatant, collect the bacterial cell pellet, rinse the bacterial cell once with sterile phosphate buffer (pH 7.0), and obtain the bacterial sludge of the 3878 strain.

[0114] Streptococcus thermophilus 3881 strain stored in glycerol tubes was inoculated in its optimized medium at an inoculation amount of 1%, cultured in a 43°C incubator for 24 hours, and after 3 generations of activation, inoculated in a 10L fermenter f...

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Abstract

The invention relates to the technical field of fermented food, and discloses streptococcus thermophilus, a sleep-aiding fermented milk base material rich in GABA, a lactic acid bacteria beverage and a preparation method. The raw materials of the fermented milk base material comprise a direct vat set leavening agent and milk liquid; and the direct vat set leavening agent comprises lactobacillus helveticus 3878 and streptococcus thermophilus 3881. The lactic acid bacteria beverage comprises the following raw materials in percentage by mass: 20-60% of the fermented milk base material, 4-10% of a sweet substance, 0.01-0.1% of a stabilizer, 0.05-0.5% of edible essence, 0.05-0.1% of a sour substance and the balance of water. The lactobacillus helveticus 3878 and the streptococcus thermophilus 3881 are compounded and fermented together to prepare the fermented milk base material and the lactic acid bacteria beverage, the two strains can play a synergistic role, and compared with a single strain, the lactic acid bacteria beverage have higher gamma-aminobutyric acid production efficiency, so that the obtained lactic acid bacteria beverage has higher GABA content and better sleep aiding effect.

Description

technical field [0001] The invention relates to the technical field of fermented food, in particular to a streptococcus thermophilus, a fermented milk base material rich in GABA, a lactic acid bacteria beverage and a preparation method. Background technique [0002] With the acceleration of the pace of life and the increase of pressure in contemporary society, various forms of sleep quality problems are gradually spreading among the population. A global sleep epidemiological survey conducted by WHO found that 27% of people worldwide have sleep quality problems, and 600 million people in my country also suffer from insomnia problems, and the insomnia population is gradually getting younger. Poor sleep quality is a risk factor for many diseases, and is closely related to hypertension, coronary heart disease, diabetes, etc. It can also affect people's mental health, causing fatigue, anxiety, depression, and increasing negative emotions. [0003] γ-aminobutyric acid (GABA for s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23C9/13A23C9/123C12P13/00C12R1/46C12R1/225
CPCC12N1/20C12P13/005A23C9/1234A23C9/1238A23C9/1307A23C9/1322A23V2400/147A23V2400/249Y02P60/87
Inventor 周晴晴李理陈苏王晓君王健李言郡余腾斐朱珺
Owner HANGZHOU WAHAHA TECH
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