Method for evaluating quality of folium artemisiae argyi

A technology of quality assessment and artemisia leaf, applied in the field of quality assessment of mugwort leaves, can solve the problems of complex pre-treatment process of mugwort leaves, affecting the accuracy of mugwort leaf quality evaluation, incomplete quality evaluation of mugwort leaves, etc., to speed up the quality evaluation process, pertinence and practicality The effect of good performance and accurate quality assessment

Inactive Publication Date: 2020-01-07
GUANGZHOU JINAN BIOMEDICINE RES & DEV CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in the existing detection technology of Artemisia argyi, the content of volatile components such as eucalyptol is often used as the detection index of Artemisia argyi, which does not involve the detection of other volatile components and inorganic trace elements in Artemisia argyi. The quality evaluation is not comprehensive, which affects the accuracy of the quality evaluation of mugwort leaves
In addition, in the existing detection technology, the pretreatment process of Artemisia argyi is relatively complicated

Method used

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  • Method for evaluating quality of folium artemisiae argyi
  • Method for evaluating quality of folium artemisiae argyi
  • Method for evaluating quality of folium artemisiae argyi

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Experimental program
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Effect test

Embodiment 1

[0034] S1 Measurement of main component content: headspace-gas chromatography-mass spectrometry is used to quantitatively measure the main components in Artemisia argyi. The specific steps are as follows:

[0035] S11 Preparation of the test solution: crush Artemisia argyi (e.g., Nanyang Artemisia) in advance until it passes through a No. 4 sieve (ie 65 mesh), mix well, accurately weigh 0.8g of Artemisia argyi powder into a 20ml headspace bottle, add 7ml of ultra-pure water, seal the headspace bottle, shake well, and let it stand for 30 minutes to obtain the test solution.

[0036] Preparation of S12 reference substance solution: Accurately weigh the reference substances eucalyptol, thujone, L-camphor, and natural borneol, add methanol to dissolve, and prepare mixed reference substances of 0.01, 0.02, 0.04, 0.1, 0.2, and 0.4 mg / ml Solution, measure 1000ul mixed reference substance solution to 20ml headspace bottle, add 10ml water, seal the headspace bottle, shake well, let sta...

Embodiment 2

[0059] The embodiment of the present invention is basically the same as embodiment 1, the difference is:

[0060] When measuring the content of the main components in step S1, in the step S11, crush the leaves of mugwort in advance until they pass through a No. Pure water, seal the headspace bottle, shake well, and let it stand for 25 minutes to obtain the test solution.

[0061] In the step S12, accurately weigh the reference substances eucalyptol, thujone, L-camphor, and natural borneol, add methanol to dissolve, and prepare mixed reference substance solutions of 0.01, 0.02, 0.04, 0.1, 0.2, and 0.4 mg / ml , Measure 1000ul of the mixed reference substance solution into a 20ml headspace bottle, add 10ml of water, seal the headspace bottle, shake well, and let stand for 25min to obtain the reference substance solution.

[0062] When measuring the content of inorganic elements in step S2, in the step S211, pulverize the leaves of mugwort in advance to pass through No. 5 sieve (i...

Embodiment 3

[0064] The embodiment of the present invention is basically the same as embodiment 1, the difference is:

[0065] When measuring the main component content in step S1, in the step S11, crush the leaves of mugwort to 70 mesh in advance, mix well, accurately weigh 1.0g of leaves of mugwort powder into a 20ml headspace bottle, add 12ml of ultrapure water, and seal the headspace bottle, shake well, and let it stand for 20 minutes to obtain the test solution.

[0066] In the step S12, accurately weigh the reference substances eucalyptol, thujone, L-camphor, and natural borneol, add methanol to dissolve, and prepare mixed reference substance solutions of 0.01, 0.02, 0.04, 0.1, 0.2, and 0.4 mg / ml , Measure 1200ul of the mixed reference substance solution into a 20ml headspace bottle, add 13ml of water, seal the headspace bottle, shake well, and let stand for 20min to obtain the reference substance solution.

[0067] When measuring the content of inorganic elements in step S2, in the...

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Abstract

The invention provides a method for evaluating quality of folium artemisiae argyi. The method comprises the following steps of step S1, weighing 0.8 to 1.2g of folium artemisiae argyi powder into a headspace bottle, adding 7 to 12ml of water, sealing the headspace bottle, shaking uniformly, standing for 20 to 40min, obtaining a test article solution, preparing a reference solution, measuring the reference solution, the test article solution and 1ml of gas in the headspace bottle, and injecting the reference solution, the test article solution and the gas into a gas chromatography-mass spectrometer, analyzing a chromatogram-mass spectrogram, and obtaining the contents of various main components in the folium artemisiae argyi; step S2, weighing 0.15 to 0.35g of folium artemisiae argyi powderinto a digestion tank, adding 8 to 10ml of nitric acid, standing overnight, digesting, removing acid, keeping the volume constant, shaking uniformly, filtering, obtaining a test sample solution, testing the test sample solution by using an inductively coupled plasma source mass spectrometer, and obtaining the contents of inorganic elements; and step S3, performing quality evaluation on the foliumartemisiae argyi according to the contents of various main components and the contents of various inorganic elements in the folium artemisiae argyi, so that a quality evaluation result of the foliumartemisiae argyi can be obtained more comprehensively and accurately.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine preparations, in particular to a method for evaluating the quality of mugwort leaves. Background technique [0002] Artemisia argyi is the dry leaf of Ai (scientific name: Artemisia argyi Levl.et Vant) of Compositae. It is recorded in the "Huangdi Neijing". In recent years, with the extensive development of Artemisia argyi research, it has been found that the medicinal effect of Artemisia argyi is based on a large number of volatile components, flavonoids, tannins, polysaccharides and other complex chemical components. In addition, studies have shown that its The inorganic trace elements contained are the active ingredients of Aiye Lixue. [0003] At present, the existing detection standards of Artemisia argyi can be used, such as the varieties included in the Pharmacopoeia or the quality control industry standards of related preparations, existing patents, etc., to evaluate...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/16G01N21/71G01N1/44
CPCG01N1/44G01N21/71G01N30/02G01N30/16
Inventor 王一飞郭玉英马婧任哲
Owner GUANGZHOU JINAN BIOMEDICINE RES & DEV CENT
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