Volvariella volvacea spore mutagenesis method

A technology for spores and straw mushrooms, which is applied in the field of mutagenesis of straw mushroom spores, can solve the problems of unsatisfactory mutagenesis effect and difficult spore walls with dense structure, and achieve the effect of reducing the probability of reverse mutation.

Inactive Publication Date: 2020-01-14
CHANGSHU INSTITUTE OF TECHNOLOGY
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Problems solved by technology

[0006] Plasma with high energy can pass through the cell wall and cell membrane of vegetative cells more smoothly, but it is more difficult for the spore wall with dense structure and increased thickness (this chara

Method used

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  • Volvariella volvacea spore mutagenesis method
  • Volvariella volvacea spore mutagenesis method
  • Volvariella volvacea spore mutagenesis method

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Embodiment Construction

[0025] (1) Take straw mushrooms for enterprise production (Jiaxing Shi Jinqiu Ecological Agriculture Co., Ltd.) as the object, such as figure 1 shown, collect its scattered spores as figure 2 Shown: Select fresh, mature straw mushrooms with intact cap edges and process them on an ultra-clean workbench. Use 75% alcohol cotton to sterilize the surface of the cap. During this process, do not wet the gills with alcohol. Use a laboratory sterile knife to cut off the flesh on the surface of the cap to make the cap thinner. This process protects the shape of the cap. And cut off the stipe; put it in a sterile plate padded with black paper, put it in a constant temperature incubator at 28 °C, let the gill spores fall freely, when the spore print deposited on the black paper in the petri dish is obviously clear pink When the color is marked, stop the collection of spores; remove the cap on the ultra-clean workbench, and the collection is generally about 10-12 hours. If the time is to...

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Abstract

The invention discloses a volvariella volvacea spore mutagenesis method which comprises the following steps of (1) collecting volvariella volvacea spores; (2) scraping the spores into sterile water bya small sterile knife, performing dilution, and stopping dilution when the spore concentration reaches 10<6> to 10 <8>/mL; (3) before working, firstly starting a cooling water circulation machine, then opening a helium gas valve, enabling the gas input pressure to be between 0.15 MP and 0.2 MP, performing ultraviolet sterilization for 20 min, firing sterilized slide glass for 30s on an alcohol lamp, and putting the fired sterilized slide glass into a sterile empty plate; and (4) taking 1 mL of a spore suspension, adding 143 mL of sterile glycerol with the concentration being 5 percent, performing uniform shaking, recording the liquid as mother liquid, sucking 10 muL of the mother liquid, dripping the mother liquid onto the slide glass in a suspended way, transferring the sterile plate into an ARTP operation room, taking and putting the slide glass onto a corresponding mutagenesis position by a pair of special sterile tweezers, clamping an EP tube containing 1 mL of sterile water underthe mutagenesis position, and performing mutagenesis under the condition of the input power being 100 W, the jet flow being 10 SLW and the irradiation mutagenesis distance being 2 mm. The volvariellavolvacea spore mutagenesis method has the advantages that the back mutation probability caused by passage can be fundamentally reduced.

Description

technical field [0001] The invention relates to a method for mutagenesis of straw mushroom spores, which is used for cultivating new varieties of straw mushrooms with excellent traits, and can also be applied to other edible mushroom varieties. Background technique [0002] Straw mushrooms are the main export mushrooms in my country. They are very nutritious and delicious, and are deeply loved by consumers. Straw mushroom has low production cost and short cycle, and is suitable for growth in high temperature and high humidity environment. However, straw mushroom is not resistant to low temperature. At 4°C, mycelium will autolyse and die within 48h, and fruit body will soften, autolyze, and die within 24h. Browning and even rot, which seriously hindered the development of straw mushroom industry (Sun Xiaohong, 2006. Journal of Mycology, 25(1):88-93). Therefore, cultivating new varieties of straw mushroom with low temperature resistance and high biological efficiency is an ur...

Claims

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Application Information

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IPC IPC(8): C12N13/00C12R1/645
CPCC12N13/00
Inventor 姚璐晔冀宏蔡信琴马荧荧张迪
Owner CHANGSHU INSTITUTE OF TECHNOLOGY
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