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Grh gene relevant with blattella germanica crust development, dsRNA of gene and preparation method and application of dsRNA

A German cockroach and epidermis technology, applied in the field of genetic engineering, can solve the problems of food stale, contaminated food, and spread of diseases.

Active Publication Date: 2020-01-17
GUANGMEIYUAN R & D CENT KEY LAB OF INSECT DEVELOPMENTAL BIOLOGY & APPL TECH SOUTH CHINA NORMAL UNIV MEIZHOU +1
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AI Technical Summary

Benefits of technology

This patented technology uses specific genes from Japanese cocks or other insects called grhn (Groundnut) gene to create small chemical substances like synthetic double stranded DNA (dsDNA). These tiny compounds then bind specifically to certain proteins found only inside these plants but outside their cells where they could harm them. By controlling how much bacteria have been introduced during plant infested areas by adding these special materials onto crops grown without causing damage it becomes possible to prevent damaging diseases such as root rot caused by fungi.

Problems solved by technology

This patented technical problem addressed in this patents relates to finding ways to effectively kill cokorrosensing insects that live at home or around us without causing damage to crops or other plants being cultivated nearby.

Method used

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  • Grh gene relevant with blattella germanica crust development, dsRNA of gene and preparation method and application of dsRNA
  • Grh gene relevant with blattella germanica crust development, dsRNA of gene and preparation method and application of dsRNA
  • Grh gene relevant with blattella germanica crust development, dsRNA of gene and preparation method and application of dsRNA

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Embodiment 2

[0045] Embodiment two of the present invention is: the application of dsRNA (in vivo experiment of German cockroach):

[0046] In order to verify the influence of the dsRNA targeting the Grh gene prepared by the present invention on the development of the epidermis of the German cockroach, it is implemented by injecting the dsRNA into the German cockroach. CK, which does not target any gene of Blattella germanica, was used as a negative control for dsRNA.

[0047] The steps are as follows: select 5-year-old (N5) and 6-year-old (N6) German cockroaches that have just molted and raise them, place them on the microscope dissecting table after anesthesia at low temperature on the second day after their molting, and then use microinjection Methods The dsRNA targeting the Grh gene was injected into the abdomen of the German cockroach according to a certain dose, and the injection was performed once in total. After the injection, the effect of dsRNA treatment on the molting of the Ger...

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Abstract

The invention discloses a Grh gene relevant with blattella germanica crust development, dsRNA of the gene and a preparation method and application of the dsRNA. The nucleotide sequence of the Grh geneis shown as SEQID No.1, and the dsRNA is double stranded RNA consisting of the nucleotide sequence as shown in SEQID No.2 and the nucleotide sequence which is backward complementary with the nucleotide sequence as shown in SEQID No.2. An RNAi jamming technique is adopted, and through dsRNA synthesis, crust developmental relevant gene Grh is targetedly silent to influence exuviate of roaches, andbased on blocking or destroying roach exuviate, the purpose of roach control is achieved. A new molecular target is provided for pest control.

Description

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Claims

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Application Information

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Owner GUANGMEIYUAN R & D CENT KEY LAB OF INSECT DEVELOPMENTAL BIOLOGY & APPL TECH SOUTH CHINA NORMAL UNIV MEIZHOU
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