Determination method of Panax notoginseng in Tianqi Dieda Fengshi ointment

A detection method and rheumatism technology, which can be used in measuring devices, instruments, scientific instruments, etc., can solve the problems of difficult extraction of active ingredients, excluding quantitative testing, complex formulas, etc., and achieve less residues in formula matrix, less interfering components, and repeatability. good stability

Active Publication Date: 2020-08-04
GUANGZHOU BAIYUNSHAN PHARM CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current quality inspection standard for Tianqi Dieda Fengshi Ointment is only the standard contained in the sixth volume of traditional Chinese medicine preparations (WS3-B-1114-92) issued by the Ministry of Standards, which can only detect Tianqi Dieda Rheumatism Licorice and rhubarb in ointment, and this standard can only be tested qualitatively, not including quantitative tests
In addition, the formula of Tianqi Dieda Fengshi Ointment is complicated, and the extraction of active ingredients is difficult. At present, there is no plan for quality control of it based on the content of a certain active ingredient.

Method used

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  • Determination method of Panax notoginseng in Tianqi Dieda Fengshi ointment
  • Determination method of Panax notoginseng in Tianqi Dieda Fengshi ointment
  • Determination method of Panax notoginseng in Tianqi Dieda Fengshi ointment

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Embodiment 1

[0055] This embodiment provides a method for detecting the content of Panax notoginseng in Tianqi Dieda Fengshi ointment, comprising the following steps:

[0056] 1. Preparation of reference solution

[0057] Take an appropriate amount of the reference substance of notoginseng saponin R1, weigh it accurately, add methanol to make a reference solution containing 0.1 mg per 1 ml, and obtain it.

[0058] 2. Preparation of the test solution

[0059] (1) Take 12.5g of Tianqi Dieda Fengshi Ointment, weigh it accurately, add 10ml of water, 25ml of dichloromethane, heat and reflux in a water bath until Tianqi Dieda Fengshi Ointment dissolves;

[0060] (2) Put it in a separatory funnel while it is still hot, let it stand still to separate the water layer, add 25ml of petroleum ether (60-90°C) (to remove impurities), heat and reflux in a water bath for 10min;

[0061] (3) Place in a separatory funnel again, stand to separate the water layer, extract 4 times (20ml, 20ml, 20ml, 10ml) wi...

Embodiment 2

[0122] This embodiment provides a method for detecting the content of Panax notoginseng in Tianqi Dieda Fengshi ointment, comprising the following steps:

[0123] 1. Preparation of reference solution

[0124] Take an appropriate amount of the reference substance of notoginseng saponin R1, weigh it accurately, add methanol to make a reference solution containing 0.1 mg per 1 ml, and obtain it.

[0125] 2. Preparation of the test solution

[0126] (1) Take 10g of Tianqi Dieda Rheumatism Ointment (referred to as test product 3-2), weigh it accurately, add 7ml of water, 20ml of dichloromethane, heat and reflux in a water bath until Tianqi Dieda Rheumatism Ointment dissolves;

[0127] (2) Put it in a separatory funnel while it is still hot, let it stand still to separate the water layer, add 20ml of petroleum ether (60-90°C), and heat to reflux in a water bath for 10min;

[0128] (3) Place in a separatory funnel again, let stand to separate the water layer, extract 5 times with w...

Embodiment 3

[0140] The present embodiment is the variation example of embodiment 1, with respect to embodiment 1, changes include in the preparation of need testing solution:

[0141] In step (1), Tianqi Dieda Fengshi ointment 15g, accurately weighed, add water 15ml, dichloromethane 30ml;

[0142] In step (3), extract with water-saturated n-butanol 3 times (20ml, 20ml, 20ml).

[0143] The effect of this embodiment is the same as that of Embodiment 2.

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Abstract

The invention relates to a method for detecting the content of pseudo-ginseng in pseudo-ginseng contusion and rheumatism ointment. The method comprises the steps of: preparation of a reference substance solution; preparation of a test solution: taking the pseudo-ginseng contusion and rheumatism ointment, and adding water and dichloromethane for extraction; stewing the extracting solution obtainedin the previous step, separating a water taking layer, and adding petroleum ether for extraction; stewing the extracting solution obtained in the previous step, separating the water taking layer, andadding n-butyl alcohol for extraction; washing the extract liquor obtained in the previous step by using an ammonia test solution to obtain a n-butyl alcohol solution; evaporating the n-butyl alcoholsolution obtained in the previous step to dryness to obtain residues, dissolving the residues in methanol to prepare the test solution; and chromatographic detection. According to the method providedby the invention, a suitable test product preparation process is adopted to extract notoginsenoside R1 from the pseudo-ginseng contusion and rheumatism ointment for liquid chromatographic analysis, formula matrix residue in the test solution is few, the loss of the notoginsenoside R1 is little, the interference elements are few, and an accurate content value can be obtained at proper washing gradient. Furthermore, the content of pseudo-ginseng is characterized by the notoginsenoside R1, so that the specificity is high, the method is not interfered by the chemical components of other medicinalmaterials, and the repeat stability is good.

Description

technical field [0001] The invention relates to the technical field of component detection of traditional Chinese medicine preparations, in particular to a method for detecting the content of notoginseng in Tianqi Dieda Fengshi ointment. Background technique [0002] Tianqi Dieda Fengshi Ointment is a compound preparation of traditional Chinese medicine. It is composed of 25 traditional Chinese medicines such as Sanqi, Dangguiwei, Drynaria, Dipsacus, Peach Kernel, Duhuo, and Clematis. , the effect of expelling wind and dampness, mainly treating soft tissue injuries, rheumatic lumbago and other diseases. The composition of the product is reasonable and the curative effect is definite. [0003] As an important traditional Chinese medicine compound preparation, the quality inspection of Tianqi Dieda Fengshi Ointment is very important. However, the current quality inspection standard for Tianqi Dieda Fengshi Ointment is only the standard contained in the sixth volume of tradit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74
Inventor 陈溪吴志红陈贤安
Owner GUANGZHOU BAIYUNSHAN PHARM CO LTD
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