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Content measurement method for verbascoside in Weixuening preparation

A technology of verbascoside and a determination method, which is applied in the field of quality detection of traditional Chinese medicines, and can solve problems such as the determination of the content of verbascoside components.

Inactive Publication Date: 2020-02-14
GUIZHOU XINBANG PHARMACEUTICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the existing quality inspection method of Jiejiekebi Pills does not measure the content of verbascoside. In order to further improve the quality inspection standards of Jiejiekebi Pills and ensure the quality of its medicines, the method of establishing the content of verbascoside in Jiejiekebi Pills is established. The content determination project is very necessary

Method used

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  • Content measurement method for verbascoside in Weixuening preparation
  • Content measurement method for verbascoside in Weixuening preparation

Examples

Experimental program
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Effect test

Embodiment 1

[0087] Embodiment 1 of the present invention: a method for determining the content of verbascoside in Weixuening preparations, the steps are as follows:

[0088](1) Chromatographic conditions and system suitability test: octadecylsilane bonded silica gel is used as filler; methanol is used as mobile phase A, 0.02% phosphoric acid aqueous solution is used as mobile phase B, gradient elution, elution condition: 0 ~5min: A:B=25:75; 5~35min: A:B=25~35:75~65; 35~70min: A:B=35~50:65~50; 70~80min: A:B =50~25: 50~75; 80~110min: A:B=25:75; the detection wavelength is 203nm, the flow rate is 0.8mL / min, the column temperature is 25°C, and the number of theoretical plates is not less than 3000;

[0089] (2) Preparation of the reference substance solution: take an appropriate amount of the verbascoside reference substance in the Weixuening preparation, accurately weigh it, and add armor to make a solution containing verbascoside per 1mL, to get final product;

[0090] (3) Preparation of ...

Embodiment 2

[0092] Embodiment 2 of the present invention: a method for determining the content of verbascoside in Weixuening preparations, the steps are as follows:

[0093] (1) Chromatographic conditions and system suitability test: octadecylsilane bonded silica gel is used as filler; methanol is used as mobile phase A, 0.2% phosphoric acid aqueous solution is used as mobile phase B, gradient elution, elution condition: 0 ~5min: A:B=25:75; 5~35min: A:B=25~35:75~65; 35~70min: A:B=35~50:65~50; 70~80min: A:B =50~25: 50~75; 80~110min: A:B=25:75; the detection wavelength is 203nm, the flow rate is 0.9mL / min, the column temperature is 30°C, and the number of theoretical plates is not low based on the peak of verbascoside at 3000;

[0094] (2) Preparation of reference substance solution: take an appropriate amount of verbascoside reference substance, weigh it accurately, add methanol to make every 1mL containing verbascoside Rg 1 0.1mg solution, ready;

[0095] (3) Preparation of the test so...

Embodiment 3

[0097] Embodiment 3 of the present invention: a method for measuring verbascoside content in Weixuening preparations, the steps are as follows:

[0098] (1) Chromatographic conditions and system suitability test: use octadecylsilane bonded silica gel as filler; use methanol as mobile phase A, 1% phosphoric acid aqueous solution as mobile phase B, gradient elution, elution condition: 0 ~5min: A:B=25:75; 5~35min: A:B=25~35:75~65; 35~70min: A:B=35~50:65~50; 70~80min: A:B =50~25: 50~75; 80~110min: A:B=25:75; the detection wavelength is 203nm, the flow rate is 1.0mL / min, the column temperature is 35°C, and the number of theoretical plates is not less than 3000;

[0099] (2) Preparation of reference substance solution: take an appropriate amount of verbascoside reference substance, weigh it accurately, add methanol to make every 1mL containing verbascoside Rg 1 0.1mg solution, ready;

[0100] (3) Preparation of the test solution: Accurately weigh 5-20g of Weixuening preparation o...

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Abstract

The invention discloses a content measurement method for verbascoside in Weixuening preparation. The content measurement method is a high performance liquid chromatography method taking a verbascosidereference substance as contrast, methyl alcohol as a mobile phase A and 0.02-2% phosphoric acid solution as a mobile phase B to elute in a gradient way. By the content measurement method, rapid, accurate, high-reproducibility and high-recycling rate measurement on content of the verbascoside in the Weixuening preparation can be achieved.

Description

technical field [0001] The invention relates to a content determination method of verbascoside in Weixuening preparation, which belongs to the technical field of traditional Chinese medicine quality detection. Background technique [0002] Weixuening traditional Chinese medicine preparation is composed of tiger shellfish, fried white peony root, Agrimony, rehmannia glutinosa, Caulis Spatholobus, Rehmannia glutinosa, Eclipta chinensis, and Radix Pseudostellariae. It has the effects of nourishing yin and nourishing blood, clearing away heat and cooling blood. For bleeding caused by yin deficiency and blood heat; thrombocytopenia sees the above syndromes. [0003] Weixuening traditional Chinese medicine preparation contains rehmannia glutinosa, which is mainly used for clearing heat and promoting body fluid, cooling blood to stop bleeding, clearing heat and cooling blood, nourishing yin and promoting body fluid, enriching blood and nourishing yin, benefiting essence and filling...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/32G01N30/34G01N30/36G01N30/74
CPCG01N30/02G01N30/06G01N30/32G01N30/34G01N30/36G01N30/74G01N2030/027G01N2030/324
Inventor 李苹孔令忠欧凌云杨淙
Owner GUIZHOU XINBANG PHARMACEUTICAL CO LTD
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