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Method for measuring content of gentiopicroside in arthralgia-treating pill

A kind of technology of gentiopicroside and determination method, which is applied in the field of quality inspection of traditional Chinese medicines, and can solve the problems of gentiopicroside content determination and the like

Inactive Publication Date: 2020-02-14
GUIZHOU XINBANG PHARMACEUTICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the existing quality inspection method of Joint Kebi Pills does not measure the content of gentiopicroside. The content determination project of gentiopicroside is very necessary

Method used

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  • Method for measuring content of gentiopicroside in arthralgia-treating pill

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Embodiment 1

[0091] Embodiment 1 of the present invention: the method for measuring the content of gentiopicroside in Joint Kebi Pills comprises the following steps:

[0092] (1) Chromatographic conditions and system suitability test: octadecylsilane bonded silica gel is used as filler; methanol: 0.05% phosphoric acid aqueous solution = 30:70 is used as mobile phase; column temperature is 35°C; detection wavelength is 224nm; The flow rate is 0.9mL / min; the number of theoretical plates should not be less than 4000 based on the peak of gentiopicroside;

[0093] (2) Preparation of reference substance solution: take an appropriate amount of gentiopicroside reference substance, accurately weigh it, add methanol to make a solution with a concentration of 0.2 mg / mL, and obtain it;

[0094] (3) Preparation of the test solution: Accurately weigh 3 g of Joint Kebi Pills, accurately add 100 mL of methanol, weigh the weight, extract by ultrasonic or heating under reflux for 45 min, or extract by micro...

Embodiment 2

[0096] Embodiment 2 of the present invention: the method for measuring the content of gentiopicroside in Joint Kebi Pills comprises the following steps:

[0097] (1) Chromatographic conditions and system suitability test: octadecylsilane bonded silica gel is used as filler; acetonitrile:0.05% glacial acetic acid=20:80 is used as mobile phase; column temperature is 35°C; detection wavelength is 224nm; The flow rate is 0.8mL / min; the number of theoretical plates calculated based on the peak of gentiopicroside should not be less than 4000;

[0098] (2) Preparation of reference substance solution: take an appropriate amount of gentiopicroside reference substance, accurately weigh it, add methanol to make a solution with a concentration of 0.2 mg / mL, and obtain it;

[0099] (3) Preparation of the test solution: Accurately weigh 3 g of Joint Kebi Pills, accurately add 100 mL of methanol, weigh the weight, extract by ultrasonic or heating under reflux for 45 min, or extract by microw...

Embodiment 3

[0101] Embodiment 3 of the present invention: the method for measuring the content of gentiopicroside in Joint Kebi Pills comprises the following steps:

[0102] (1) Chromatographic conditions and system suitability test: octadecylsilane bonded silica gel is used as filler; methanol:0.05% glacial acetic acid=35:65 is used as mobile phase; column temperature is 40°C; detection wavelength is 240nm; The flow rate is 1.5mL / min; the number of theoretical plates calculated based on the peak of gentiopicroside should not be less than 4000;

[0103] (2) Preparation of reference substance solution: take an appropriate amount of gentiopicroside reference substance, accurately weigh it, add methanol to make a solution with a concentration of 0.2 mg / mL, and obtain it;

[0104] (3) Preparation of the test solution: Accurately weigh 3 g of Joint Kebi Pills, accurately add 100 mL of methanol, weigh the weight, extract by ultrasonic or reflux for 45 min, or extract by microwave for 6 min, mak...

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Abstract

The invention discloses a method for measuring content of gentiopicroside in an arthralgia-treating pill. The method is a high performance liquid chromatography method which takes a gentiopicroside reference substance as a reference and takes methanol, water, 0.01-5% phosphoric acid aqueous solution, 0.01-5% glacial acetic acid and 0.01-0.5% formic acid aqueous solution in a ratio of (50-70): (50-30) or acetonitrile, water, 0.01-5% phosphoric acid aqueous solution, 0.01-5% glacial acetic acid and 0.01-0.5% formic acid aqueous solution in a ratio of (20-50): (80-50) as a mobile phase. The method disclosed by the invention is high in specificity, high in precision, high in repeatability, high in recovery rate, high in stability and accurate in measurement result, the aim of effectively controlling the quality of medicines is achieved, and the stability of the product quality and the safety and effectiveness of clinical medication are ensured.

Description

technical field [0001] The invention relates to a method for determining the content of gentiopicroside in Joint Kebi Pills, which belongs to the technical field of traditional Chinese medicine quality detection. Background technique [0002] Joint Kebi Pills have the effects of expelling wind and cold, activating collaterals and relieving pain. For arthritis, sore limbs, poor stretching and other diseases. Modern pharmacological experiments have proved that Joint Kebi Pills have antispasmodic effect on rabbit ileum; it has analgesic, sedative and anti-inflammatory effects at the same time: Pharmacological studies have shown that Joint Kebi Pills can significantly prolong the time caused by hot plate method in mice. The pain reaction time of the animals is shown to have obvious analgesic effect. [0003] Among them, gentiopicroside, as an important active ingredient in Joint Kebi Pills, has choleretic, anti-inflammatory, stomach-invigorating, and blood pressure-lowering ef...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/36G01N30/74B01J20/281
CPCG01N30/02G01N30/06G01N30/34G01N30/36G01N30/48G01N30/74G01N2030/027
Inventor 杨淙李苹孔令忠欧凌云
Owner GUIZHOU XINBANG PHARMACEUTICAL CO LTD
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