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Method for measuring content of secondary alkaloid in nicotine

A determination method and alkaloid technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of inaccurate detection technology and achieve the effect of improving sensitivity and accurate and effective detection results

Pending Publication Date: 2020-02-25
SHANGHAI NEW TOBACCO PRODUCTS RESEARCH INSTITUTE CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the detection methods are separated by classical liquid chromatography, combined with ultraviolet detection technology for qualitative analysis, but such detection technology is not accurate

Method used

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  • Method for measuring content of secondary alkaloid in nicotine
  • Method for measuring content of secondary alkaloid in nicotine
  • Method for measuring content of secondary alkaloid in nicotine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Detection target of the sample to be tested: the sample to be tested is high-concentration nicotine, targeting mesmin, codinine, 2S-nicotine-1-oxide, R,S-nornicotine, R,S- Anatabine, S-Pseudine and diene nicotine were determined.

[0080] Reagents: deuterated cotynine, cotynine, 2S-nicotine-1-oxide, R,S-nornicotine, R,S-anatabine, mesmin, S-basine, diene Seven secondary alkaloid standard samples of nicotine, acetic acid, ammonia water, formic acid (LC-MS grade), methanol (HPLC grade), high-purity water.

[0081] Equipment: High performance liquid chromatography (Agilent 1290) tandem triple quadrupole mass spectrometer (Agilent 6495)

[0082] 1. Preparation of standard working solution

[0083] 1) Internal standard solution

[0084] Cordinine-D 3 The mother liquor was diluted with methanol to a volume of 1 μg / mL for later use.

[0085] 2) Working solution

[0086] The concentration of the mother solution of the standard substance and the concentrations of the stand...

Embodiment 2

[0138] The selection of analysis conditions and parameters in Example 2 is basically the same as in Example 1, except that the chromatographic column is ACQUITY UPLC BEH C18 (particle size 1.7 μm, inner diameter 2.1 mm, length 100 mm).

Embodiment 3

[0140] The selection of analysis conditions and parameters in Example 3 is basically the same as in Example 1, except that the mobile phase B is a formic acid-acetonitrile solution with a volume fraction of formic acid of 0.025%.

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Abstract

The invention provides a method for measuring content of secondary alkaloid in nicotine. A method of liquid chromatography-multi-stage mass spectrography is used for accurately measuring content of low-content secondary alkaloid in the high-purity nicotine, wherein the secondary alkaloid comprises one or more of myosmine, cotinine, 2S-nicotine-1-oxide, R, S-nornicotine, R, S-anatabine, S-neonicotine and nicotyrine. The measuring method provided in the invention is suitable for measurement of content of secondary alkaloid in high-concentration and even high-purity nicotine, high-concentration nicotine and low-content secondary alkaloid are separated through liquid chromatography, and then qualitative and quantitative analysis is performed on alkaloid through mass spectrography, so that a detection result is accurate and effective.

Description

technical field [0001] The invention relates to a method for determining alkaloids, in particular to a method for determining the content of secondary alkaloids in nicotine. Background technique [0002] Tobacco products are showing a trend of diversified development, especially the new tobacco products represented by e-cigarettes are expanding in market size worldwide and have broad application prospects. [0003] Nicotine is the most abundant alkaloid in tobacco and smoke. It can act on the central nervous system and is the most important physiologically active component of tobacco. It is also the most important reason why people consume tobacco to achieve physical and psychological satisfaction. Therefore, in order to achieve a physiological feeling similar to real cigarettes, electronic cigarettes often contain different concentrations of nicotine (ie nicotine). The purity of nicotine will affect the sensory experience of electronic cigarettes to varying degrees. Using ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/89G01N30/54
CPCG01N30/54G01N30/89
Inventor 杨菁汪阳忠王天南费婷
Owner SHANGHAI NEW TOBACCO PRODUCTS RESEARCH INSTITUTE CO LTD
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