Method for in-situ microbial remediation of petroleum-contaminated soil under the assistance of heat strengthening
A technology for microbial remediation and oil pollution, which is applied in the field of in-situ microbial remediation of petroleum hydrocarbon-contaminated soil through thermal strengthening, which can solve the problems of constraints and high remediation costs, and achieve the effects of increasing availability, high remediation efficiency, and shortening remediation period
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[0022] The present invention will be further described in conjunction with specific embodiments.
[0023] Aiming at a certain oil field, the technical scheme of the present invention is adopted to carry out a pilot test of soil restoration. The pilot test site is divided into Area A, Area B and Area C. In Area A, the heat-enhanced assisted in-situ microbial remediation technical solution of the present invention is adopted. In Area B, only the in-situ microbial remediation technical solution is used. In Area C, only in-situ Technical solution for thermal desorption repair.
[0024] 1. Preparation of free bacterial solution:
[0025] Take 100mL of the RCW bacteria solution for petroleum wastewater treatment and add it to 700mL enrichment medium, and cultivate it to the logarithmic growth phase (mixing bacteria for 28 hours before irradiation, and mixing bacteria for 12 hours after irradiation). Take 60 mL of the cultured strain and centrifuge. Remove the supernatant, add ste...
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