Application and related products of human EME1 gene

A gene and application technology, applied in the application of human EME1 gene and related products, can solve the problem of no report of EME1 function
CN110863050AInactive Publication Date: 2020-03-06CANCER CENT OF GUANGZHOU MEDICAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CANCER CENT OF GUANGZHOU MEDICAL UNIV
Publication Date
2020-03-06
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the field of biological medicine research, and particularly relates to an application of a human EME1 gene serving as a target in preparing a treatment medicament of nasopharyngeal carcinoma. According to extensive and deep research, after expression of the human EME1 gene is downregulated by adopting an RNAi method, proliferation of nasopharyngeal carcinoma cells can be effectively inhibited, apoptosis is promoted, and thus a growth process of nasopharyngeal carcinoma can be effectively controlled. An siRNA provided by the invention or a nucleic acid construct containing the siRNA sequence, and lentivirus can specifically inhibit the proliferation rate of nasopharyngeal carcinoma cells, promote apoptosis of the nasopharyngeal carcinoma cells, inhibit metastasis ofthe nasopharyngeal carcinoma cells, inhibit invasion of the nasopharyngeal carcinoma cells and inhibit growth of the nasopharyngeal carcinoma, so that nasopharyngeal carcinoma is treated, and a new direction is opened up for treatment of nasopharyngeal carcinoma.
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Description

technical field

[0001] The invention belongs to the field of biomedical research, and specifically relates to the use of human EME1 gene and related products. Background technique

[0002] EME1 is an endonuclease identified as a member of the XPF endonuclease family. EME1 plays an important role in DNA repair following topoisomerase 1 inhibition and its upregulation confers resistance to multiple DNA damaging agents (doi:10.1016 / j.dnarep.2007.02.019). This protein cooperates with the methanolsulfonate-sensitive UV-sensitive 81 protein to form an endonuclease complex. The Mus81-Eme1 complex induces DNA double bond breaks near the ICL (interstrandcrosslinks, ICL) through structure-specific endonuclease activity, suggesting that the complex plays an important role in ICL repair (doi:10.1038 / sj.emboj. 7601344). Consistent with this finding, normal cells from mice deficient in the Mus81 or Eme1 genes were hypersensitive to mitomycin and cisplatin (doi.org / 10.1093 / emboj / cdg580;...

Claims

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