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Method for increasing content of secondary metabolites in vaccinium bracteatum by means of G. gigantea infection

A technology of secondary metabolites, wufanshu, applied in the fields of soil microorganisms, environment, and food additives, can solve the problems of low content of secondary metabolites in the interior, and achieve the goal of improving viability, improving water absorption, and maintaining stability. Effect

Active Publication Date: 2020-04-14
宜兴乾元黑色食品科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the content of endogenous secondary metabolites in the leaves of Wufan tree grown in a natural state is low, and needs to be further improved.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The root system of the black rice tree was sterilized by 0.01% mercury liter, washed with distilled water, soaked in 1 spore / ml G. gigantea strain culture solution for 0.01h, and the infected black rice tree was cultured in normal soil for 1 day, and the leaves were measured by HPLC method The content of orientin, a secondary metabolite of flavonoids, showed that the content of orientin increased by 0.01% compared with that before G. gigantea infection.

Embodiment 2

[0033] The root system of the black rice tree was sterilized by 0.25% KMnO4, washed with sterilized water, soaked in 250 spores / ml G. gigantea strain culture solution for 0.5h to infect, and the inoculated black rice tree was cultured in normal soil for 3 months Adopt HPLC method to measure the content of secondary metabolite 10-O-p-hydroxycinnamoyl-dihydrocrystal aglycone in its plant, the result shows that the content of 10-O-p-hydroxycinnamoyl-dihydrocrystal aglycon is higher than that of G . gigantea strains increased by 0.5% before infection.

Embodiment 3

[0035]Adopt 10 milliliters that contain 500 spores / ml G. gigantea bacterial classification culture solution, pour in the soil around the root of the black rice tree planted in soil to infect, after infecting 1 month, adopt HPLC method to measure the number of times in the black rice tree plant Raw metabolites vitexin, isorientin, orientin, quercetin-3-O-β-D-glucuronide, (trans) 10-O-p-hydroxycinnamoyl-dihydro crystal blue Aglycone, (cis) 10-O-p-hydroxycinnamoyl-dihydrocrystal aglycone, (trans) 10-O-p-hydroxycinnamoyl-chrysanthemum aglycon, (cis) 10-O - the content of p-hydroxycinnamoyl-galinagenin, the results showed that the content of vitexin increased by 0.1%, the content of isoorientin increased by 0.5%, the content of orientin increased by 0.3%, quercetin- The content of 3-O-β-D-glucuronide increased by 0.01%, the content of (trans) 10-O-p-hydroxycinnamoyl-dihydrocrystal aglycone increased by 0.02%, (cis) 10-O - The content of p-hydroxycinnamoyl-dihydrocrystal aglycone i...

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Abstract

The invention discloses a method for increasing a content of secondary metabolites in vaccinium bracteatum by means of G. gigantea infection. According to the method for increasing the content of thesecondary metabolites in vaccinium bracteatum by means of G. gigantea infection, a root system of vaccinium bracteatum is infected with G. gigantea to form a symbiotic system of vaccinium bracteatum and G. gigantea. Through mutual effects and a mutual beneficial relation which are formed between G. gigantea and soil around the root system of vaccinium bracteatum, an significant increase of the content of the secondary metabolites in vaccinium bracteatum plants is promoted. By means of the method for increasing the content of the secondary metabolites in vaccinium bracteatum by means of G. gigantea infection, the content of the secondary metabolites in the vaccinium bracteatum plants can be significantly increased, wherein the content of flavone compounds is increased by 0.01% or above, thecontent of iridoid glycoside components is increased by 0.01% or above, the content of procyanidin components is increased by 0.01% or above, and the content of other secondary metabolites is increased by 0.01% or above. By means of the method for increasing the content of the secondary metabolites of vaccinium bracteatum by means of G. gigantea infection, the content of the efficacy components,namely the iridoid glycoside components, for dyeing branches and leaves of vaccinium bracteatum can be increased, so that dyeing power of the branches and leaves of vaccinium bracteatum is improved; and the content of the flavone secondary metabolites and the content of the procyanidin secondary metabolites can be increased, so that nutritional components of black rice are increased.

Description

technical field [0001] The invention belongs to the field of soil microbes and the environment, and also belongs to the field of food additives, and relates to a method for increasing the content of secondary metabolites of acacia plants by infecting with Mycocystis maxima. [0002] technical background [0003] Gigaspora gigantea (hereinafter referred to as G. gigantea) is an Arbuscular mycorrhiza fungi. Arbuscular mycorrhizal fungi can form a symbiotic relationship with plants, but the infection of arbuscular mycorrhizal fungi is exclusive, and only when they establish symbiosis with the host plant can they complete their own life cycle. The existence of arbuscular mycorrhizal fungi plays an important role in the survival of host plants. It can significantly increase the adaptability of host plants to adversity and enhance their survival competitiveness. [0004] Black rice tree, also known as Nanzhu, is an evergreen shrub or tree of Rhododendronaceae and Vaccinium genus. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G17/00A01G7/06
CPCA01G17/005A01G7/06
Inventor 张琳付红伟
Owner 宜兴乾元黑色食品科技有限公司
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