Method for detecting human No.21 triple-chromosome and reagent box

Abstract

The present invention discloses a prenatal diagnosis method of Down's syndrome. It is characterized by using PCR to amplify at least three lock on the No.21 chromosome and observing the polymorphism of these loci. Besides, said invention also discloses the reagent kit for said invented method, including the specific primer for PCR amplification.

Description

technical field [0001] The invention relates to a method for prenatal diagnosis of Down's syndrome, in particular to a method for prenatal diagnosis of Down's syndrome using polymerase chain reaction (PCR), and also relates to a reagent for the diagnosis method of the present invention box. Background technique [0002] Down's syndrome, also known as congenital stupidity, or trisomy 21, is one of the most common chromosomal disorders. It is also the main cause of congenital mental retardation. Its clinical features are: severe mental retardation, unique facial and physical deformities (such as wide eye distance, low bridge of the nose, muscle weakness, short limbs, and penetrating hands, etc.), congenital heart disease, digestive tract malformations, etc., the incidence of leukemia much taller than the average person. [0003] The pathogenesis of the disease is that germ cells do not segregate during meiosis. The vast majority of cases are caused by the non-segregation o...

AI Technical Summary

Problems solved by technology

Because the size of the PCR product is different, even if the same primer is used, the amount of the amplified product will vary greatly, which may lead to quantitative errors and misdiagnosis of chromosomal trisomy syndrome

In addition, the instruments and equipment used in most reports are relatively expensive (such as using capillary electrophoresis equipment, or fully automatic DNA sequencers and other equipment worth hundreds of thousands of dollars), or using harmful reagents such as radioisotopes

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