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Rice sheath blight effector gene RsIA-NP8 and application thereof

A rice sheath blight fungus and gene technology, applied in the field of genetic engineering, can solve problems such as ineffective effects

Active Publication Date: 2020-05-15
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Strangely, monocot pathogens also carry NLPs genes, but their role in pathogenicity remains obscure because they do not cause cell necrosis

Method used

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  • Rice sheath blight effector gene RsIA-NP8 and application thereof
  • Rice sheath blight effector gene RsIA-NP8 and application thereof
  • Rice sheath blight effector gene RsIA-NP8 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Predict the isolation and cloning of effector genes

[0032] Under the ultra-clean workbench, use tweezers to pick a small amount of Rhizoctonia solani AG1IA cells, insert them into 50ml PDA medium, cultivate them at 28°C and 200r / m for two days, filter and collect mycelium with four layers of gauze, and use Total RNA was extracted by liquid nitrogen grinding. Using oligodT as a primer, cDNA was obtained by reverse transcription.

[0033] Primers were designed according to the predicted sequences, and the primer sequences are as follows:

[0034] Forward primer: 5'-ATGCGTTTTGCGACCGTAGC-3' (SEQ ID NO: 3);

[0035] Reverse primer: 5'-GATGGCAACGCACGATTGTT-3' (SEQ ID NO: 4).

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PUM

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Abstract

The invention provides a rice sheath blight effector gene RsIA-NP8 and application thereof. A nucleotide sequence of thte gene is shown in SEQ ID NO:1; and an amino acid sequence of a gene-encoded protein is shown in SEQ ID NO:2. According to the rice sheath blight effector gene RsIA-NP8 and the application thereof disclosed by the invention, through cloning of the rhizoctonia solani effector geneand function analysis of the gene, revelation on specific interaction between the rice sheath blight and rice varieties as well as an evolved molecular mechanism are facilitated.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a rice sheath blight effector gene RsIA-NP8 and an application thereof. Background technique [0002] Rice sheath blight is a worldwide fungal disease of rice caused by Rhizoctonia solani Kühn, and is known as one of the three major diseases of rice. The disease has a wide distribution range. As far as the global rice-growing regions are concerned, it occurs frequently in rice-growing countries in Asia, America, and Africa. The disease was once the most important rice disease in the southern rice region of the United States. The fungus causes ribbon blight of corn, rice, and sorghum, cotton damping-off, aerial blight and stem rot of mung beans and soybeans, sheath rot, cabbage heart rot, potato black spot, and leaf rot. Face blight. The ability of sheath blight to infect a variety of crops is related to the secretion of effector molecules. This molecule...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/31C07K14/37C12N15/82A01H5/00A01H6/46
CPCC07K14/37C12N15/8282
Inventor 郑爱萍魏苗苗刘尧王爱军马丽李平
Owner SICHUAN AGRI UNIV
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