62 results about "Heart rot" patented technology

The invention discloses bacillus velezensis HNt7 with broad-spectrum antibacterial activity as well as a preparation method and application. The antagonistic bacterium bacillus velezensis HNt7 has a number of CCTCC (China Center for Type Culture Collection) M 2018022. The preparation method comprises the following steps: A, selecting a bacillus velezensis HNt7 strain individual bacterial colony under a sterile condition and inoculating into a culture medium; adjusting the temperature and carrying out shake culture; B, filling a fermenting solution into each bottle and adding individual bacterial colony bacterium liquid according to the ratio; putting the bottle into a large-amplitude constant-temprature freezing shaking table and carrying out the shake culture; C, separating the antagonistic bacterium: immersing with alcohol with concentration and rinsing with sterile water; carrying out dark culture in a culture box and transferring a grown bacterial colony into a new culture medium and carrying out lineation separation and purification on the bacterial colony; D, after separating and purifying, obtaining the bacillus velezensis HNt7. The bacterium is used for preparing a medicinefor treating or preventing pear fruit rot disease. The strain has a remarkable disease-prevention effect and the prevention effect reaches 70.76 percent or more. After the bacillus velezensis HNt7 isapplied to pear trees, advanced defoliation of the pear trees is remarkably inhibited and the tree vigor is easy to enhance.

The invention provides a talaromyces flavus, and also relates to the application of the talaromyces flavus in the inhibition of a variety of plant pathogens. The preservation number of the talaromyces flavus qw12 is CGMCC5067, the talaromyces flavus colony is round, the texture is flocculent and fluffy or flocculent, and the front of the colony is yellow; the conidiophores have penicilli, and the spore is spherical; the primordium is nearly cylindrical, big and curved; the gymnothecium is spherical or nearly spherical, the ascus is spherical, and the ascospore is spherical or nearly sphericaland unicellular. The antagonistic bacterium provided by the invention can effectively inhibit the growth of valsa sordida, rhizoctonia solani, corn sheath blight fungi, curvularia lunata, colletotrichum gloeosporioides, fusarium graminearum, alternaria alternate, cryphonetria parasitica, colletotrichum nicotianae and other plant pathogens, effectively prevent plant diseases, reduce the dosage of chemical pesticide and the accumulation of the chemical pesticide in soil and help to increase the quality and yield of economic crops in China, and therefore economic and social benefits are remarkable.

The invention discloses a foliar fertilizer for controlling various physiological diseases of crops. The foliar fertilizer is prepared by urea, calcium superphosphate, potash fertilizer, zinc sulfate, magnesium sulfate, borax, ferrous sulfate, duck feather, carboxymethylcellulose, 35% of sulfuric acid, lime, celangulin, sanguinarine, Azadirachtin and other materials. The foliar fertilizer has full nutrition, high concentration and obvious fertilizer efficiency. The plants grow vigorously after being applied with the foliar fertilizer, the yield of the crops is obviously increased, the quality is better, and the disease and lodging resistance is greatly improved. In addition, the safe and pollution-free botanical pesticides contained such as celangulin, sanguinarine and Azadirachtin can effectively reduce the insect and diseases of the crops, and do not pollute the environment and crops. The foliar fertilizer can be used for controlling the root rot of sweet potato, heart rot of maize, withered tip of maize leaf, maize stripe mosaic disease, cotton red leaf blight, cotton leaf cast, red leaf and yellow leaf cast of mint, yellow leaf dwarf of beans, rice red blight and the like, and the effect is remarkable.

The present invention relates to a strawberry tree twig and leaf extract germicide and its production method. Said production method includes the following procedures: drying and pulverizing, reflux dissolving, centrifugal separation and vacuum concentration to obtain extract, then using (by weight portion) 30-80 weight portions of above-mentioned extract, 20-50 portions of solvent and 10-20 portions of adjuvant to prepare germicide emulsifiable concentrate or using 20-40 portions of the above-mentioned extract, 10-20 portions of solvent, 10-20 portions of adjuvant and 30-50 portions of deionized water to prepare them into germicide microemulsion. Said germicide can be mainly used for controlling cucumber anthrax bacteria, apple putrescence disease-producing germ and banded sclerotial blight of rice plant, etc.

The invention relates to the technical field of biological pesticide, and specifically relates to a composite biological pesticide, which is composed of the following raw materials: castor-oil plant powder, rapin, raw ginger, cranesbill, sweet wormwood, common cnidium fruit, thunder god vine, and a synergist. The composite biological pesticide can kill inspects, and can also be used as a plant disease prevention agent. The composite biological pesticide has a control effect of 95% to 100% on larva of insects with chewing mouth parts or piercing-sucking mouth parts if being used as a pesticide, and has a control effect of 90% to 100% on diseases such as plant virus disease, rotten disease, root-rot disease, wilt disease, scab disease, physiological disease, runt seedling, root decline, and the like, if being used as a disease prevention agent. The composite biological broad-spectrum pesticide is prepared from biological products and traditional Chinese herbals, so the pesticide can kill insects, while does not have any side or toxic effect on crops at the same time, people can safely enjoy the crops, and the crops is totally harmless for human health. Furthermore, the quality of agricultural products is prominently improved at the same time, so the composite biological broad-spectrum pesticide is suitable for vast popularization and application.

The invention discloses a method for planting strawberries. An appropriate field is selected for planting the strawberries, fertilizers are spread on the field, strawberry seedlings are planted on the field, fertilizers are applied to the strawberry seedlings in a timing mode within certain time and guarantee lighting, film covering is performed on strawberry strong seedlings in November, so that the strawberries are obtained in the next year, and Chinese herbal medicine insect repellant is spread in the period of film covering to perform insect disease prevention. Through the method for planting the strawberries, Chinese herbal medicine components included in the Chinese herbal medicine insect repellant fully have a good prevention effect on a rotting disease appearing in the strawberry planting, all the components in the Chinese herbal medicine insect repellent are mutually synergized and supplemented and have the effects of microorganism resistance, virus resistance, parasite resistance, fungus resistance and immunity enhancement, and therefore taste and edible performance of the strawberries are guaranteed.

The invention discloses an agricultural composition containing naphthylacetic acid and strobilurin fungicide; wherein the strobilurin fungicide is one of pyraclostrobin, trifloxystrobin, picoxystrobin, coumoxystrobin, and azoxystrobin, and the weight ratio of naphthylacetic acid to strobilurin fungicide is 50:1-1:100, preferably 1:1-1:60. The agricultural composition can be made into agriculturally-acceptable suspending agent, wettable powder, soluble powder, and paste. The formula of the agricultural composition is reasonable; the agricultural composition has a good effect on controlling fruit tree rot, and can promote wound healing and reduce the relapse rate; compared with the mono-component preparation, the provided agricultural composition has a prominent control effect and an remarkable synergetic effect, the cost is low, the safety is high and the agricultural composition meets the safety requirements of agricultural preparations. The agricultural composition has a prominent control effect on rotten diseases of apple trees and pear trees.

The invention provides a smearing preparation for controlling fruit tree rotten disease. The smearing preparation is prepared from the following raw materials in part by weight by the conventional method: 40 to 200 parts of 30 percent Xin bacteria amine mother liquor, 45 to 100 parts of auxin sodium nitrophenolate, 750 to 840 parts of film-forming agent polyvinyl alcohol and 5 to 20 parts of glycol. The invention is characterized in that: the smearing preparation has a broad-spectrum sterilization function, and is low in toxicity, safe, reliable, economical and practical, residue is avoided, and the medicine and rehabilitation are integrated; and the worldwide problem of controlling fruit tree rotten disease is solved.

The invention provides a high-activity multi-functional special pole coating agent for fruit trees and belongs to the technical field of planting of forest woods. The special pole coating agent provided by the invention is prepared by taking organic mineral materials, a high-activity microbial preparation, major elements, medium elements, trace elements, an anti-freezing and moisture-keeping material, amino acids, fulvic acid, chitin, a preparation for preventing and controlling plant diseases and insect pests, a synergistic auxiliary and water as raw materials. Due to the addition of the high-activity biological preparation, the major, the medium and the trace elements, the amino acids, fulvic acid, the organic mineral materials, the anti-freezing and moisture-keeping material, the material for preventing and controlling the plant diseases and the insect pests and the synergistic material, the biological activity of the product is greatly improved, and various nutrients required for the fruit trees are balanced. The special pole coating agent is directly sprayed on trunks and branches of the fruit trees, so that the using efficiency of fertilizers and pesticides is improved, the growth of the fruit trees and the swelling of the fruits are promoted, the effects of preventing the plant diseases and the insect pests, preventing freezing and promoting growth are enhanced, the effect of improving the quality of the fruits is significant and the special pole coating agent particularly has a significant effect of preventing and controlling rot which is so called a cancer for apple trees.

The invention provides an endophytic fungus-Talaromyces flavus Y28 with a biocontrol effect. The Talaromyces flavus Y28 is separated from a high-rot-resistance pear tree variety, and a preservation number of the Talaromyces flavus Y28 is CGMCC No.11918. The invention further provides an application of the Talaromyces flavus Y28 to prevention and control of a fruit tree rotten disease. As proved by tests, the antibacterial rate of the Talaromyces flavus Y28 in fungus in-vitro opposing antibacterial tests can be up to 70.50 percent, and the antibacterial rate in annual branch in-vitro secondary screening tests is 55.54 percent, so that the Talaromyces flavus Y28 is a good biocontrol strain for preventing and controlling the pear tree rotten disease.

The invention discloses a fertilizer, and especially relates to an organic water-soluble fertilizer containing amino acid microbes, and a preparation method thereof. The fertilizer is prepared through mainly using 7-13 parts by weight of a Trametes trogii Berkeley mycelium, 7-13 parts by weight of a poplar mycelium, 7-13 parts by weight of a Piptoporus soloniensis mycelium, 7-13 parts by weight of a Laetiporus sulphureus Laetiporus sulphureus, 3-5 parts by weight of calcium sulfate, 3-5 parts by weight of chitin, and 7-13 parts by weight of leaf humus. The fertilizer contains nutritional element substances comprising microbial fungi, fungal polysaccharides and the like needed in the growth process of crops. The fertilizer has the advantages of reduction of the application amounts of chemical fertilizers and pesticides, production increase, income increase, crop quality improvement, disease and pest damage prevention, and rotting disease and leaf spot disease prevention when the fertilizer is applied to the crops. When the fertilizer is applied to the crops through water washing or spray, the fertilizer allows nutritional components to be rapidly transferred to the crops, makes the crops have enough nutrition, promotes the high yield of the crops, improves the crop quality, and prevents the disease and pest damage.

The invention discloses a household orchid planting technology. The household orchid planting technology comprises: in planting, compost prepared from leaf mold or peat soil, sand and ceramsite in a ratio of 5:2:3 is selected as pot soil appropriately; in watering, orchid is watered to slightly wet as the orchid is fond of coolness; in fertilizing, thick fertilizer is avoided as the orchid requires less amount of fertilizer and extremely low concentration; in the aspect of a propagation method, a plant division method is adopted for propagation; in pest control, common diseases of the orchid comprise a rotten disease and black spot, and common injurious insects of the orchid comprise thrips and red spiders; in flower growing temperature, potted orchid is moved into a room when air temperature is cooled to 5 DEG C; and in a flower growing environment, the potted orchid is placed on an indoor antique shelf, a desk or window in the room to be appreciated.

The invention provides a method for grafting other species on a stiff branch of a tree. The method aims to solve the problems that in the prior art, after grafting, branch rotten disease is prone to occurring to a trunk and a backbone branch, a shoot is prone to being blown and broken by wind at the very year of grafting, shaping difficulty is large and pruning workload is large in one to three years after the grafting, and manpower is wasted for removing a large number of main branches and the backbone branches. The method comprises the four steps of (a) selection and remaining of a stock, (b) handling of the stock, (c) selection and manufacturing of a cion and (d) grafting.

The invention discloses paenibacillus sp. CL01 and an application thereof. The paenibacillus sp. CL01 is preserved in China General Microbiological Culture Collection Center on June 28, 2020, and thepreservation number of the strain is CGMCC No.20161. The strain is wide in antibacterial spectrum and strong in antibacterial ability, has a strong inhibition effect on common pathogenic fungi on peartrees such as colletotrichum gloeosporioides, alternaria alternata, alternaria alternata and valsa ceratosperma, and also has a strong antagonistic effect on phytopathogens such as rhizoctonia solani, botrytis cinerea, sclerotinia sclerotiorum and phytopathogen. The biocontrol microbial inoculum prepared from the strain has a relatively high biocontrol effect on fungal diseases of fruits and leaves on pear trees. The composition can replace or reduce the use of chemical pesticides, improves the safety of food and ecological environment, has good economic and social benefits, and has a very wide application prospect in biological prevention and control of plant diseases.

The invention discloses a farm chemical composition containing thymol. The effective components of the composition are thymol, carvacrol and menthol. The composition disclosed by the invention is a pure botanical natural farm chemical, has no harvesting interval when in use, and has no harm to human beings and pathogenic bacteria natural enemies; when diseases are treated, adverse reactions (suchas growth suppression, flower bud differentiation influence and the like) of plants cannot be caused; the composition has a strong killing effect on gray mold and powdery mildew of various crops, andalso has a very good treatment effect on some other crop diseases such as cowpea sooty mould, tomato leaf mold, tomato bacterial leaf spot, cucumber angular leaf spot, cucumber gummosis, watermelon shade skin disease, pepper stem rot, strawberry root rot and pineapple heart rot.

The rot disease is similar to the cold and can outbreak to attack the fruit tree at any time particularly in the peak season of spring. The rot disease can also outbreak in the autumn, which mainly depends on the amount of the precipitation in a year. The fundamental method for preventing and treating the rot disease is to make great effort to prevent and treat the rot disease in the infection period mainly in the autumn during production. The method for preventing and treating the rot disease of the fruit tree in autumn comprises the following steps: (1) tracking and treating the recurrent disease spots in the fruit tree growing season with Fubiqing, 843 healing agent and the like; (2) carefully and completely scaling the recurrent disease spots and eliminating bacteria source tissues; (3) pruning the fruit tree in winter and clearing the rotten branches; (4) preventing and treating the rot disease in autumn and in spring selectively for 2 to 3 consecutive years so as to fully prevent the fruit tree from suffering from the rot disease and restore the appearance of the whole orchard; (5) strengthening the disease resistance of the fruit tree by fertilizing and loading the fruit tree; and (6) scaling the dying tissues in the rhytidome, the dried stakes and the insect-plague branches in autumn according to the characteristics that the pathogenic bacterium spores propagate with rainwater in summer and spreading 50 times of 4 percent agricultural antibiotic 120 of the fruit tree liquid medicine on the fruit tree so as to eliminate the parts where the pathogenic bacteria exit for latent infection.

The invention discloses an in-vitro verification method for apple rot disease resistance. The method comprises the following steps: (1) culturing conidiophores of Valsa mali Miyabe et Yamada at 20-30 DEG C for 6-24 hours to obtain the bourgeoned conidiophores; (2) inoculating the isolated branch with the bourgeoned conidiophores in step (1), and culturing at 20-30 DEG C under the relative humidity of 60-100%; and after culturing for 5-16 days, taking out the branches, and identifying the resistance. The verification method for apple rot disease resistance uses the cultured spores in the bourgeoning state to infect the branches according to the characteristics of plant disease resistance research development and rot disease occurrence. The invention conforms to the law of infection of Valsa mali Miyabe et Yamada, has the advantages of moderate pathogenicity and uniform disease occurrence. The method can verify the resistance of apple varieties to the rot disease, and has the advantages of convenience, high speed, simple and easy realization, reliable result and the like.

The invention provides an insect-resistant agent for growing watermelons. The insect-resistant agent is prepared from, by weight, 23-32 parts of mugwort, 23-32 parts of cortex moutan radicis, 23-32 parts of euphorbia humifusa willd bark, 11-17 parts of longan kernels, 3-8 parts of semen sterculiae lychnophorae, 3-8 parts of lailaihui, 2-9 parts of radix et rhizomarhei and 6-8 parts of a plant leaf extracting solution. The insect-resistant agent can kill eggs of pests, emit specific smells used for dispelling mosquitoes and flies, inhibit breeding of harmful bacteria, fully ensure robust growth of the watermelons and prevent the watermelons from heart rotting and watermelon vines from rotting and lesion.

The invention relates to the technical field of identification of pathogenic microorganisms, in particular toprimers for identifying pathogenic microorganisms of apple tree branch and trunk rot and a disease degree grading method. The identification primers have good specificity, can conduct specific amplification on genomic DNA of three strains of methylobacterium brachiatum, sphingomonas aquatilis and aureobasidium pullulans in robust fruit trees, two strains of valsa mali and botryococcus brauni in diseased fruit trees, and four strains of sagonosporopsis lupini, cladosporium allicum, didymella keratinophil and cladosporium delicatulum in withered fruit tree after disease, are free of primer dimers and non-specific amplification, can be used for detecting a diseased state and a diseased degree of apple tree branch rot in an incubation period with a good effect, further judges the diseased state and the diseased degree of the apple tree branch rot, helps establish a detection and grading standard of the diseased degree, and provides a technical support for early detection and scientific prevention and treatment of the apple tree branch rot.

Disclosed is a method for preventing and treating pineapple heart rot. Nutritional agents are used, heart filling treatment is performed on pineapple plants for 50-60ml / plant before coming of the low temperature, and the nutritional agent formula is 0.1% to 0.4% of a borax solution and 1% to 3% of a calcium chloride solution. According to the method, the formula is simple, the pertinence is high, pineapple heart rot can be prevented and treated effectively, and time and labor are saved; the pineapple plant cold resistance and disease resistance can be improved apparently, and the yield is improved.

The invention discloses a light environment regulation and control method for reducing a heart rot rate of lettuces in a plant factory. The method comprises the step that in the cultivation process ofthe lettuces in the plant factory, green light is added for irritation to reduce the heart rot rate of the lettuces. According to the light environment regulation and control method for reducing theheart rot rate of the lettuces in the plant factory, in the cultivation process of the lettuces, on the basis of matching of red light and blue light, an appropriate proportion of green light is addedfor light environment regulation and control cultivation, so the yield can be significantly increased, and the heart rot rate of the lettuces is reduced.

The invention provides a cuttage planting method simple and easy in operation and high in survival rate and aims to overcome the defect of low survival rate of a vegetative propagation technique for artemisia ordosica cuttage planting in the prior art. The method has advantages that on the one hand, by mixing of raw materials including coarse sand, urea, composted farmyard manure, peat and the like in a cuttage planting process, the survival rate in artemisia ordosica cuttage is increased; besides, by control of adding quantities of various materials in appropriate ranges, optimal synergisticeffects of the materials can be achieved, and the survival rate in artemisia ordosica cuttage can be further increased; finally, by adding of carbendazol wettable powder and Bordeaux mixture for aftertreatment in a cuttage process, bactericidal and pesticidal effects in the cuttage process can be further enhanced, the rot disease and root rot can be better prevented and treated, influences of disease and pest damage are reduced, disease resistance of plants is improved, and the survival rate in cuttage is increased.

The invention discloses a pesticide used for preventing and treating the rot disease of fruit trees. The pesticide is prepared from the following raw materials by weight: 10 to 12 parts of pre-fractionation oil, 10 to 20 parts of light oil, 10 to 15 parts of a pesticide emulsifier, 5 to 10 parts of copper sulphate, 10 to 15 parts of kerosene, 5 to 10 parts of zinc sulfate, 10 to 15 parts of ammonium molybdate, 5 to 10 parts of a binder and 20 to 30 parts of water. The pesticide provided by the invention can kill and inhibit fruit tree rot disease germs, effectively infiltrate into the inner layers of fruit tree barks, form an effective water-proof protective layer on the surface and a protective film on wounds of the barks and effectively resist attack by outside germs. The pesticide can resist washing by heavy rain; the drug effect of the pesticide lasts for more than 50 d; and the pesticide has a rot disease germ inhibition rate of more than 90%, can substantially reduce influence of residual on fruit and pollution to the environment and is a nontoxic, nuisanceless and novel pesticide.

The invention discloses a quick healing method for a walnut tree large saw opening. The method includes six steps of cutting off large branch retaining piles, deeply cutting phloem into three equal parts, peeling phloem from xylem, covering the saw opening, adhering phloem to the saw opening, and shading and wrapping. Compared with the traditional saw opening which is simply sealed with a paint orsealing glue, the saw opening is not easy to be infected by bacteria, the wound healing speed is faster, the healing degree is better, scars are not easy to form on parts such as trunk and main branches, and rot disease is not easy to generate. Compared with the traditional method, the method can effectively solve the problems of slow healing of the large saw opening, rot caused by bacterial infection of the saw opening, dehydration of xylem exposed by the saw opening and tree growth declining caused by decay of the trunk pith due to bacterial infection, and lay a foundation for the healthy growth, yield and quality improvement of the tree body in the later period.

The invention discloses an application of brassinosteroid in prevention and treatment of apple tree canker. The brassinosteroid is brassinolide, 24-epibrassinolide or 28-epibrassinolide. The brassinosteroid can significantly improve the disease resistance of apple trees to the rot disease and reduce the occurrence degree of the rot disease; and meanwhile, operation is easy and convenient, the use amount of chemical pesticide can be reduced, and remarkable economic and ecological environment benefits are achieved.

The invention relates to a method for preventing orchid from rotten disease. The method comprises the following steps: (1) timely carrying out replacement of flowerpot and soil, and shearing off and intensively burning off all tissues of a diseased part; (2) cleaning and disinfecting cut with clean water, coating the cut with sulfur powder, and putting the cut at a cool ventilated place to dry; (3) potting again after the cut shrinks, not watering or spraying water to the cut within 3-5 days, and spraying the cut once with a solution which is prepared by adding water, 500-800 times amount of 50% thiram, into the 50% thiram; (4) isolating the rescued diseased or infected orchid plant from other orchid; (5) when the diseased or infected orchid plant grows normally, placing the diseased or infected orchid plant and other orchid together. The method disclosed by the invention is scientific and unique, simple in operation by adopting shearing, cleaning and disinfecting and isolating methods, and capable of effectively preventing spread of the rotten disease, so that the orchid rotten disease is fundamentally controlled.

The invention provides application of trichoderma harzianum or fermentation broth thereof in treatment and / or prevention of pitaya tree rot disease. According to the invention, the research results show that a trichoderma harzianum fermentation broth has a very good prevention and treatment effect on pitaya tree rot disease caused by the diffusion of Scytalidium dimidiatum conidium, can be used for preparing various preparations for preventing or treating pitaya fruit tree rot diseases, and provides a novel, efficient and environment-friendly biocontrol agent source for preventing and treating pitaya tree rot disease.

The invention relates to the technical field of vegetation control, in particular to a film-forming straw coating fertilizer and a preparation method thereof. Comprising the following raw materials in parts by weight: 1-2 parts of polyurethane, 1-3 parts of calcium bicarbonate, 1-3 parts of chitosan, 2-5 parts of compound amino acid, 1-2 parts of organic matters, 0.5-2 parts of microbial agent metabolites, 1-1.5 parts of nutrient ions, 0.2-1 part of copper sulfate, 0.5-1 part of a chelating agent and the balance of water. The problems that an existing fertilizer is single in function, and preparation cannot be treated are mainly solved. According to the invention, plants can be more robust, root growth is promoted, and the immunity of the plants is improved. And diseases such as rot, gummosis and dry rot of plants can be effectively treated. Meanwhile, the healing speed of plant wounds can be increased, and the anti-freezing and cold-resistant effects can also be achieved. The fertilizer is mainly used for fertilization treatment of fruit trees and other large plants with trunks.

The invention discloses a stone salt oil mixture which comprises 50 parts of water, 5 parts of quick lime, 4 parts of sulfur powder, 1.5 parts of salt and 3 parts of purified animal oil. The preparation method comprises: heating water to 20 DEG C, and adding quick lime to prepare lime water for later use; adding water into sulfur powder to dilute the sulfur powder into paste, slowly adding the paste into lime water, continuously performing boiling and stirring to fully mix the mixture, adding water again after the water is completely evaporated, and stopping heating until the color of the mixed liquid is dark brown; and adding purified animal oil into the mixed liquid, continuously performing stirring until the animal oil is completely dissolved, performing cooling, adding salt, and uniformly performing stirring to obtain the stone salt oil mixture. The stone salt oil mixture prepared by the invention is a comprehensive medicament integrating physical prevention and control and chemical prevention and control, has a protective medicament integrating contact killing, adhesion, decay, freezing injury prevention, sunburn resistance and scour prevention, and is simple to use. Pathogens of various rot diseases and various insect pests can be controlled.