28 results about "Heart rot" patented technology

The invention discloses streptomyces sp. SK-2 and an application thereof in prevention and treatment of white cone rot. The streptomyces sp. SK-2 is separated from rhizosphere soil of cedarwood soil, the biocontrol bacterium can remarkably inhibit the occurrence area of the white cone rot disease under the indoor condition, the prevention effect reaches 68% or above, and potential development and application value is achieved.

The invention relates to the technical field of plant disease-resistant gene identification and genetic engineering, in particular to a pyrus betulaefolia lectin receptor-like kinase gene sequence and application of the pyrus betulaefolia lectin receptor-like kinase gene sequence to improvement of apple and pear rot disease resistance. The pyrus betulaefolia canker-resistant gene sequence provided by the invention is used for improving the ability of resisting saprophytic fungus canker. The pyrus betulaefolia anti-disease gene provided by the invention is obtained by cloning from a pyrus betulaefolia L-LecRKs family for the first time, the function and possible action mechanism of the anti-disease gene PbeL-LecRK-S.4 in the aspect of disease resistance are further studied, and a theoretical basis is provided for breeding of pyrus betulaefolia for disease resistance. According to the invention, a pFGC5941-PbeL-LecRK-S.4 overexpression vector and a PTRV2-PbeL-LecRK-S.4 silence vector are constructed for the first time, and the pFGC5941-PbeL-LecRK-S.4 overexpression vector and the PTRV2-PbeL-LecRK-S.4 silence vector are introduced into pyrus betulaefolia ('Duli-G03') cells through transient expression verification of Huangguan pear fruits and agrobacterium tumefaciens-mediated transformation. The disease-resistant response of a pyrus betulaefolia disease-resistant gene PbeL-LecRK-S.4 overexpression suspension cell line and wild pyrus betulaefolia suspension cells after inoculation of the rot disease is studied. The pyrus betulaefolia anti-disease gene PbeL-LecRK-S.4 provided by the invention can obviously improve the resistance of pyrus betulaefolia to valsa ceratosperma.

The application provides a high-temperature germination and drought after rehydration indoor incubation flower bud method capable of improving crocus sativus L. yield and quality. Compared with a traditional two-stage cultivation method, the method initiates a high-temperature cumulative dormancy breaking method during crocus sativus L. indoor cultivation flowering period, not only promotes crocus sativus L. early flowering, but also increases crocus sativus L. flowering number, advances root system development time by two months, significantly prolongs corm vegetative growth period, and improves daughter corm yield. Meanwhile, the method can effectively increase crocus sativus L. stigma length, weight, improve crocus sativus L. glycoside content, enhance color, fragrance and taste intensity, thereby significantly improving crocus sativus L. stigma yield and overall quality. In addition, the method significantly reduces crocus sativus L. corm rot disease occurrence through high-temperature treatment and the bacteriostatic effect of quicklime, and greatly improves comprehensive economic benefits of crocus sativus L. production.

The invention provides a composition for preventing and treating rot of pear trees as well as a preparation method and application of the composition, and belongs to the technical field of pesticide preparation. The composition is prepared from osthole and tebuconazole. The biological source bactericide cnidium lactone and the chemical bactericide tebuconazole are combined for preventing and treating the bergamot pear tree rot disease or inhibiting the bergamot pear tree rot disease, the effect of preventing and treating the bergamot pear tree rot disease is improved, and the synergistic interaction effect is achieved. The method for preparing the composition is simple and easy to implement. According to the composition, the use of a chemical agent tebuconazole is reduced, a way for improving the environment and reducing pollution is provided, and a basis is provided for sustainable and long-term development of a method for preventing and treating the rot diseases of the bergamot pear trees.

The invention relates to the technical field of microorganisms and agriculture, and particularly discloses talaromyces sp. P85, a product containing the talaromyces sp. P85 and application of the talaromyces sp. P85. A functional microorganism capable of effectively preventing and controlling pineapple heart rot is separated and screened from healthy pineapple plant rhizosphere soil, and is identified as talaromyces sp. After the talaromyces sp. Is prepared into a fungicide, the disease-resistant and growth-promoting effects of plants can be remarkably improved, so that the talaromyces sp. Can be used for creating a novel biological prevention and control product for efficiently preventing and controlling pineapple heart rot.

The invention discloses a rapid and efficient pear and apple canker resistance evaluation method, and relates to the technical field of horticultural plant disease resistance evaluation.The method comprises the following steps of S1, test branch preparation, S2, pathogenic bacterium culture, S3, contact method inoculation and S4, resistance evaluation.The method can accurately evaluate the canker resistance of pear and apple resources, and the method has the advantages of being high in practicability and high in practicability. The method has the advantages of simple operation, no need of punching or preparation of fungus cakes, simplified operation steps, completion of inoculation of 100-120 parts of materials within 2 hours, efficiency more than 10 times of that of a traditional punching method, suitableness for rapid screening of large-scale germplasm resources, suitableness for canker resistance evaluation of different varieties of pears and apples, germplasm resources and filial generations, and wide application prospect. And a technical support is provided for disease-resistant variety breeding, resistance gene mining and green prevention and control.

The present application relates to the field of microbiological technology, and particularly relates to a walnut decay disease biocontrol bacterium Achromobacter insuavis P52 and application thereof.The Achromobacter insuavis is Achromobacter insuavis P52, which is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.28849.A strain of Achromobacter insuavis is isolated from a pot seedling of Populus trichocarpa, and the strain has a significant antagonistic effect on a walnut decay disease pathogenic fungus Cytospora chrysosperma, with an inhibition rate of 62.4%, and an in-vitro prevention and treatment effect on walnut branch decay disease of 76%.The Achromobacter insuavis in the present application has a significant prevention and treatment effect on walnut decay disease, and has a good application prospect.

The present application relates to a kind of Trichoderma fermentation liquor and preparation method thereof.The Trichoderma fermentation liquor is the fermentation liquor of Trichoderma sulphureum DH4-3, and the preservation number of Trichoderma sulphureum DH4-3 is CGMCC No.40704.The preparation method of the above-mentioned Trichoderma fermentation liquor includes the following steps: Trichoderma sulphureum DH4-3 is inoculated into culture medium and fermented.The Trichoderma fermentation liquor has inhibitory effect on 12 kinds of tested plant pathogenic fungi, such as poplar bark rot fungus, nursery wilt fungus, blueberry branch wilt fungus, apple rot fungus, cucumber sclerotium blight fungus, apple fruit rot fungus, melon vine cutting fungus, rice malignant worm fungus, red bean anthracnose fungus, tobacco brown spot fungus, tobacco target spot fungus and Pythium aphanidermatum, and has the advantages of good control effect, wide inhibitory spectrum and wide application range.

The invention discloses a compound microorganism for preventing and treating chestnut fruit rot and application of the compound microorganism. The compound microorganism is composed of trichoderma atroviride, bacillus siamensis and bacillus velezensis, and is constructed by directionally screening strains with broad-spectrum inhibition effect on seven core pathogenic bacteria from Chinese chestnut fruit endophytes and verifying compatibility. The method has the advantages that the compound flora synergistically prevents and controls diseases through direct antagonism and induction of plant system resistance, and the defensive enzyme activity and the content of total polyphenol and total flavone of Chinese chestnuts are remarkably improved; according to the compound microorganism, the indoor control effect reaches 90% (the morbidity is reduced), the diseased fruit rates of Chinese chestnut fruits treated in a chestnut garden are reduced by 60% and 42.86% respectively after the Chinese chestnut fruits are stored for 30 days and 120 days, the effectiveness and the stability are obviously higher than those of a single strain, the compound microorganism can be introduced in the flowering period or sprayed in the storage period, use is convenient and flexible, and an efficient and stable biocontrol strategy is provided for green prevention and control of the Chinese chestnut fruit rot.

The invention belongs to the technical field of gene engineering, and relates to application of a valsa ceratosperma virulence gene in plant disease control. The invention discloses a valsa pear tree valsa pyri virulence gene VpCYP38, the CDS region sequence of the valsa pear tree valsa pyri virulence gene VpCYP38 is shown as SEQ ID NO: 1, and the virulence of V. pyri is reduced by knocking out the valsa pear tree valsa pyri virulence gene VpCYP38 in V. pyri. The invention further constructs a dsRNA medicament based on the virulence gene VpCYP38 of the valsa ceratosperma, and the dsRNA medicament is targeted to the virulence gene VpCYP38 of the valsa ceratosperma in V. pyri through an exogenous spraying method and is used for preventing and treating plant diseases caused by V. pyri in host plants.

This invention relates to a topical agent for preventing and treating poplar canker and its application method. The topical agent, by weight percentage, comprises the following ingredients: 0.1-0.2% pyraclostrobin; 1-3% wetting and dispersing agent; 0.2%-0.8% thickener; 2-4% antifreeze agent; 0.01%-0.3% micronutrient fertilizer; 0.1%-0.3% defoamer; and the balance being deionized water. Before application, first use a scraper to clean the ulcerated areas on the poplar branches and trunks, thoroughly scraping away the rotten tissue, extending 2-3 cm beyond the diseased area on both the upper and lower surfaces. Then, evenly and promptly apply the topical agent to the treated wound surface, cover it with breathable non-woven fabric, and then apply another layer of topical agent over the non-woven fabric, ensuring close adhesion to the branches and trunk. The topical agent of this invention is simple to use, highly effective, and can effectively control canker, reduce the impact on poplar growth, and achieve efficient prevention and treatment of poplar canker.

The application discloses application of medicago acid in preparation of an anti-plant-fungus biological source medicine and belongs to the technical field of plant disease prevention and treatment. The application discloses that the medicago acid has a significant inhibiting effect on the growth of valsa mali and proves that the medicago acid can improve the expression of resistance genes in a plant body and activate the defense response of the plant body, thereby improving the resistance of the plant to fungi. The application provides a new medicine resource and a theoretical basis for developing a new type of plant disease-resistant agent, fills the vacancy of anti-fungal terpenoid compounds and provides a new idea for biological prevention and treatment of rot disease of Xinjiang wild fruit forest, cultivated apple orchard and other economic fruit orchard.

This invention discloses a Bacillus species and its applications, belonging to the field of microbial technology. The Bacillus species is a new species of the genus Bacillus (Bacillus sp. RP12), with the strain name RP12 and preservation number CCTCC No: M20221018. The Bacillus RP12 provided by this invention not only broadly antagonizes kiwifruit canker pathogens in multiple kiwifruit producing areas in China, and can be widely used to control kiwifruit canker in major kiwifruit producing areas of China; but also broadly antagonizes various plant pathogenic fungi, and can be used to control various plant diseases such as kiwifruit postharvest rot and wheat stem rot.

The invention provides bacillus velezensis CG-6 and application of the bacillus velezensis CG-6 in biological prevention and treatment of cubilose fruit rot. The preservation number of the bacillus velezensis CG-6 is CGMCC (China General Microbiological Culture Collection Center): NO.36885. The research finds that the sterile filtrate of the strain can effectively prevent and treat the cubilose fruit rot disease.

The invention belongs to the technical field of biological control, and particularly discloses streptomyces fusiformis AK308 and application thereof. The preservation number of the streptomyces fusiformis AK308 is CGMCC (China General Microbiological Culture Collection Center) No.35050. The streptomyces fusiformis AK308 has broad-spectrum antibacterial property and can be used for biocontrol bacteria to prevent and treat various plant fungal diseases such as apple alternaria leaf spot, wheat scab, apple rot, celery spot blight, corn ear rot, wheat root rot, potato black scurf or tomato early blight. The AK308 fermentation product has a good inhibition effect on 12 kinds of pathogenic fungi such as fusarium graminearum, celery spot blight and the like, the EC50 value of Botryosphaeria berengerana and the EC50 value of Valsa cerealosperma can be as low as 0.02 mu g / mL and 0.06 mu g / mL, and the strain has a wide application value. The AK308 fermentation product has a good inhibition effect on 12 kinds of pathogenic fungi such as wheat fusarium graminearum, celery spot blight and the like, and the EC 50 value of Botryosphaeria berengerana and Valsa cerealosperma can be as low as 0.02 mu g / mL and 0.06 mu g / mL.

The application discloses a kind of for preventing and treating chestnut fruit rot disease complex microorganism and its application.The application complex microorganism is composed of Trichoderma atroviride and Bacillus siamensis, Bacillus velezensis, by from chestnut fruit endophyte directional screening to seven kinds of core pathogenic bacteria have broad-spectrum inhibitory effect strain, is constructed after compatibility verification.It is superior in that: complex microbial population is synergistically controlled disease by direct antagonism and induced plant system resistance, significantly improves chestnut defense enzyme activity and total polyphenol, total flavone content;Indoor control efficiency reaches 90% (reduces the incidence), chestnut fruit in chestnut orchard treatment is reduced 60% and 42.86% respectively in 30 days and 120 days, effective and stability are obviously stronger than single strain, and the application complex microorganism can be introduced by flowering period or sprayed in storage period, and is convenient and flexible, provides efficient, stable biocontrol strategy for chestnut fruit rot green control.

This invention discloses a strain of Streptomyces SK-2 and its application in the control of white cone rot. The Streptomyces strain of this invention (… Streptomyces SK-2 (sp.) was isolated from the rhizosphere soil of Chinese fir soil. This biocontrol bacterium can significantly inhibit the occurrence area of ​​white cone rot disease under indoor conditions, with a prevention effect of more than 68%, and has potential development and application value.

This invention belongs to the field of biological control technology, specifically disclosing a strain of *Streptomyces fusiforme* AK308 and its applications. The *Streptomyces fusiforme* AK308 provided by this invention has the preservation number CGMCC No. 35050. This strain possesses broad-spectrum antifungal activity and can be used for biocontrol of various plant fungal diseases, including apple leaf spot, wheat scab, apple rot, celery leaf spot, corn ear rot, wheat root rot, potato black scurf, and tomato early blight. The fermentation product of AK308 has good inhibitory effects on 12 pathogenic fungi, including *Fusarium graminearum* and *Fusarium graminearum*. Botryosphaeria berengeriana and Valsa ceratosperma EC 50 The concentration can be as low as 0.02 μg / mL and 0.06 μg / mL, and this strain has a wide range of applications.

The invention relates to a fruit tree three-dimensional profiling pruning device which comprises an agricultural carrier, a liquid storage container, a hollow side plate, a water pump, a hollow rotating shaft, a liquid inlet hole, a shear knife, a flow guide channel, a liquid outlet hole, a ball and a driving assembly. Sealing and shearing oil in a liquid storage container is conveyed and pumped out through a water pump, after entering a hollow side plate, the sealing and shearing oil enters a hollow rotating shaft through a liquid inlet hole and then enters a flow guide channel through the hollow rotating shaft, and a driving assembly drives a shearing knife to rotate to prune fruit tree branches on the side face of an agricultural carrier; when the shearing knife is used, the balls on the shearing knife contract inwards under the extrusion action of the fruit tree branch shearing saw bite, the shearing sealing oil flows out of gaps between the balls and the liquid outlet holes, then the balls rotate under the friction action of the shearing saw bite, the shearing sealing oil is evenly smeared on the shearing saw bite, the shearing saw bite is protected, and frostbite and rot are prevented.

The application discloses a strain capable of long-acting antagonizing poplar decay pathogenic bacteria, which is Pseudomonas azotoformans, is isolated from poplar bark, and has a preservation number of CGMCC No.33365.The strain has a bacteriostatic rate of 9.68%-36.17% on live bacteria of Cytospora chrysosperma, a poplar decay pathogenic fungus, in a plate confrontation test; artificial inoculation prevention and control experiments show that the strain has a significant prevention and control effect on poplar decay; and field experiments show that the strain has a significant long-acting prevention and control effect on poplar decay.The 16s RNA gene sequence of the strain comprises the sequence shown in SEQ ID NO.1.The application also relates to application of the strain in preparation of poplar decay prevention and control products, and a composition, a bacterial agent or a biological prevention and control agent containing the strain.

The invention provides a salt-tolerant bacillus strain Sneb2652 and application thereof, and belongs to the technical field of microorganisms. The salt-tolerant bacillus sp. Sneb2652 is preserved in China General Microbiological Culture Collection Center (CGMCC), the address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is June 18, 2025, and the preservation number is CGMCC No.34935. The salt-tolerant bacillus sp. Sneb2652 has the advantages that the salt-tolerant bacillus sp. Sneb2652 can be used for preparing the salt-tolerant bacillus sp. Sneb2652, and the salt-tolerant bacillus sp. Sneb2652 can be used for preparing the salt-tolerant bacillus sp. According to the invention, a strain of salt-tolerant bacillus with a good prevention and treatment effect on the soft rot of Chinese cabbage is screened, and the salt-tolerant bacillus also has the effects of inhibiting pectobacterium carotovorum, muskmelon bacterial fruit blotch, apple ring spot, cucumber target leaf spot, apple valsa mali, tomato early blight and sweet melon fruit rot pathogen; the germination of Chinese cabbage seeds and the growth of radicles are facilitated, and the method has good use potential and application prospect in production practice.

The invention belongs to the technical field of agricultural production, and particularly relates to application of paclobutrazol to prevention and treatment of poplar rot. According to the method, paclobutrazol serving as a plant growth regulator is directly sprayed to poplar leaves; the spraying concentration of the paclobutrazol is 100 mg / L. Results of the embodiment of the invention show that paclobutrazol is directly sprayed on poplar leaves, so that pathogenic bacteria expansion of poplar rot can be inhibited, manual operations such as pruning can be reduced, plant stress resistance can be enhanced, self disease resistance of poplar can be improved, and disease recurrence can be reduced; furthermore, a method which is not easy to generate drug resistance and is environment-friendly is provided for preventing and treating poplar rot.

The invention relates to the technical field of microorganisms and agriculture, and particularly discloses a composition containing talaromyces sp. P85 and application of the composition. Based on talaromyces P85 and bacillus P20 which are separated and screened from healthy pineapple plant rhizosphere soil, the complex microbial inoculant is prepared, the disease resistance and growth promoting effects of plants can be remarkably improved, and therefore the complex microbial inoculant can be used for creating a novel biological prevention and control product for efficiently preventing and controlling pineapple heart rot.

The invention belongs to the technical field of microorganisms, and discloses an African trichoderma harzianum, a biocontrol inoculant and application of the African trichoderma harzianum, the African trichoderma harzianum is named as African trichoderma harzianum T10, the preservation number of the African trichoderma harzianum is CGMCC No.41709, the African trichoderma harzianum is preserved in China General Microbiological Culture Collection Center on December 11, 2024, and the preservation number of the African trichoderma harzianum is CGMCC No.41709. The preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The active ingredient of the biocontrol fungicide is the African trichoderma harzianum. The African trichoderma harzianum T10 has an inhibiting effect on apple tree canker and apple ring spot, can be used for preparing a biocontrol microbial inoculum, is applied to prevention and treatment of the apple tree canker and the apple ring spot, also has a growth promoting effect on fruit trees, can be used as an active component to prepare a plant growth promoting agent, and also can be applied to degradation of canker toxin. The African trichoderma harzianum T10 has the advantages of stable control effect and environmental friendliness.

The present application provides a kind of preventing and treating malus robusta branch disease biocontrol strain HTBS01 and its screening method and application.The biocontrol strain HTBS01 belongs to Baciilus subtilis, and the preservation number is CGMCC No.24547, which can be used to prevent and treat malus robusta branch diseases such as wheel pattern disease, rough bark disease, rot disease, etc.Inhibition rate of biocontrol strain HTBS01 to malus robusta wheel pattern disease, rough bark disease pathogen is more than 90% in indoor antagonism test, and the inhibition rate to rot disease pathogen is 66.67%.Forest biocontrol test shows that the control efficiency of biocontrol strain HTBS01 to rough bark disease of malus robusta of different ages is 18%-38%, and the control efficiency to rot disease is 47%-50%.The biocontrol strain HTBS01 provided in the present application can reduce the use of pesticides, is safe and non-toxic, and realizes the dual purpose of prevention and treatment of malus robusta branch diseases.

The invention discloses a CAPE1 peptide fragment at the C terminal of PtPR1 protein and application thereof, the CAPE1 peptide fragment of the PtPR1 protein has three types, namely PtPR1f, the amino acid sequence of which is shown as SEQ ID NO.1; the amino acid sequence of the PtPR1j is as shown in SEQ ID NO. 2; the amino acid sequence of the PtPR1l is as shown in SEQ ID NO. 3. Experimental results show that the CAPE1 peptide fragments at the C terminals of the three PtPR1 proteins provided by the invention can induce the woody plant to generate immune defense reaction at lower concentration; by using the three CAPE1 peptide fragments at the C terminal of the PtPR1 protein provided by the invention, the disease resistance of the woody plant to colletotrichum gloeosporioides, valsa poplar, botryosphaeria dothidea or phytophthora parasitica can be remarkably improved, and the CAPE1 peptide fragments have no inhibition effect on the growth of various pathogenic bacteria. Therefore, the strain can be applied to prevention and treatment of woody plant diseases, a new way is provided for improving the disease resistance of the woody plants, and the strain has a wide application prospect in the aspects of green prevention and control of the woody plant diseases and the like.

The present application belongs to the technical field of fruit tree disease prevention and treatment, and particularly relates to a method for preventing and treating apple tree bark rot, comprising the following steps: S1, peeling treatment, removing the bark of the apple tree in the diseased area, and then spraying the apple tree with a fungicide; S2, bridging branches, selecting healthy branches to form a bridge across the diseased area; S3, taking the distance between the two ends of the bridging branches as the height, and applying the medicine mud along the circumference of the apple tree; S4, wrapping the medicine mud and branches with a plastic film to form an outer film; and S5, updating the medicine mud every 1-3 months. The present application applies the medicine mud to the circumference of the diseased area, uses the medicine mud to release the fungicide slowly, and makes the diseased area of the apple tree in a fungicide-wrapped environment for a long time, so as to treat the diseased area of the apple tree and hinder the spread of the rot fungus.