The present invention discloses a
malus micromalus makino
tissue culture method, which comprises: selecting
malus micromalus makino with good growth as a material, taking the sterile blade
cut block with a length of 0.5 cm and a width of 0.5 cm, the leaf stalk with a length of about 1 cm, and the stem section long strip, and uniformly inoculating into a culture medium a; after about 5 weeks, forming significant cluster buds,
cutting the cluster buds into small blocks, respectively inoculating into
culture mediums b and c, and proliferating; after four weeks, when the
cut block enters a
mass proliferation stage, carrying out transfer
inoculation of the differentiated
bud into a culture medium d, and culturing; after about 3 weeks, inoculating the root-free-like
seedling with a height of about 3 cm and strong growth into a culture medium e; after 3 weeks, growing white fibrous roots on the base portion of the
seedling; and placing the test-tube
plantlet in a ventilation place for 3 days, taking out the small
seedling,
transplanting into a mixing matrix, and continuously culturing. With the method of the present invention, a large number of the high quality
malus micromalus makino seedlings can be bred, and the
survival rate is more than or equal to 95%.