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Rapid tissue culture reproduction method for malus micromalus 'fly fire'

A technology for tissue culture rapid propagation and begonia, applied in the field of plant tissue culture, can solve the problems of long propagation cycle of fire dancing begonia seeds, inability to carry out large-scale grafting propagation, and limited popularization and application, and achieves fast propagation speed and high rooting rate. , the effect of broad market prospects

Pending Publication Date: 2019-04-12
WUHAN ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the seed propagation cycle of Fire Dance Begonia is long and the resistance is poor. Nowadays, grafting is mostly used for propagation. However, due to the small number of scions, large-scale grafting cannot be carried out, thus limiting the popularization and application of this variety.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) From April to May, at 9 o'clock in the morning on a sunny day, collect the current-year-old branches containing axillary buds from the strong branches of the mother plant of the dancing crabapple, remove the leaves, and soak them in diluted soapy water for 10 minutes. Rinse with running water for 60 minutes and set aside;

[0021] (2) Disinfect the cleaned explants with 70% alcohol on the ultra-clean workbench for 30 seconds, rinse them with sterile water for 4 times, each time for 2 minutes; then disinfect them with 0.2% mercuric chloride for 8 minutes, and rinse them with sterile water for 5 minutes. times, 2 minutes each time;

[0022] (3) Put the explants on sterilized paper after disinfection, carefully clamp them with sterile tweezers, cut them into 2cm-sized stem segments with axillary buds with sterile blades, and insert them into sterile bud induction medium; bud induction The formula of the medium is: MS medium + 1.0mg·L -1 6-BA+0.2mg·L -1 NAA+3% sucros...

Embodiment 2

[0027] (1) From April to May, at 9 o'clock in the morning on a sunny day, collect the current-year-old branches containing axillary buds from the strong branches of the mother plant of the dancing crabapple, remove the leaves, and soak them in diluted soapy water for 10 minutes. Rinse with running water for 60 minutes and set aside;

[0028] (2) Disinfect the cleaned explants with 70% alcohol on the ultra-clean workbench for 30 seconds, rinse them with sterile water for 4 times, each time for 2 minutes; then disinfect them with 0.2% mercuric chloride for 8 minutes, and rinse them with sterile water for 5 minutes. times, 2 minutes each time;

[0029] (3) Put the explants on sterilized paper after disinfection, carefully clamp them with sterile tweezers, cut them into 2cm-sized stem segments with axillary buds with sterile blades, and insert them into sterile bud induction medium; bud induction The formula of the medium is: MS medium + 1.0mg·L -1 6-BA+0.2mg·L -1 NAA+3% sucros...

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PUM

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Abstract

The invention relates to a rapid tissue culture reproduction method for malus micromalus 'fly fire'. The method comprises the following steps: selecting and cleaning explants, sterilizing the explants, carrying out primary culture and subculture, and carrying out rooting culture, thereby obtaining tissue culture seedlings of the malus micromalus 'fly fire'. By adopting the method, the tissue culture seedlings of the malus micromalus 'fly fire' are high in rooting rate, high in survival rate and rapid in reproduction speed, the reproduction coefficient is up to 5.1 or greater, the rooting rateis up to 90% or greater, and in addition, the cultured malus micromalus 'fly fire' grows strongly.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture and rapid propagation of Begonia Dancing Fire. Background technique [0002] Fire Dance Begonia is a small apple of the genus Rosaceae introduced from North America, and its parents are unknown. Small deciduous tree, the main excellent characteristics are "four reds", that is, deep red flower color, red peel, red leaf color and reddish brown branch skin, large amount of flowers, long flowering period (more than 15 days), large fruit (diameter 1.5-2.5cm ), the fruit color is red, the amount of fruit is large, and it is arranged sequentially on the branches. It has a strong ability to bear fruit year after year and has a long fruit-hanging period. After treatment, it can hang until January of the next year. First, it is an excellent tree species for viewing flowers, leaves and fruits. [0003] However, the seed propagation cycle of Fire Da...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李长林金莉宿福园裴忺张剑峰姚延兴杨守坤陈绪柏
Owner WUHAN ACADEMY OF AGRI SCI
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