Method for extracting ceramide from malus micromalus rice bran

A technology of ceramide and rice bran, which is applied in the field of enzymatic hydrolysis, can solve the problems of insufficient extraction of ceramide and low yield, and achieve the effects of high extraction rate and purity, convenient extraction and short cycle

Pending Publication Date: 2022-07-29
GUANGDONG OCEAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to solve the problems of insufficient extraction of ceramide from sea red rice bran and low yield in existing

Method used

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  • Method for extracting ceramide from malus micromalus rice bran
  • Method for extracting ceramide from malus micromalus rice bran

Examples

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Embodiment 1

[0023] This example provides an application test of extracting ceramides from sea red rice bran by using double-enzymatic enzymolysis combined with ultrasonic thermal extraction technology.

[0024] Remove impurities from the dried sea red rice bran material, pulverize and sieve to obtain 20-40 mesh rice bran raw material powder, take 100 g of sea red rice bran, add 300 mL (calculated in enzyme activity units, sphingomyelinase and cellulase) The ratio is 4:6) in the enzymatic hydrolysis solution (concentration of 0.2w / v%) of the preparation, at 35 ° C, the pH is adjusted to 5, the enzymatic hydrolysis is carried out for 60 minutes, and the filtration is performed to obtain the enzymatic hydrolysis of rice bran (water). Take 1 g of enzymatically hydrolyzed rice bran (water), add 500 mL of 75% ethanol solution, stir for 20 minutes, let it stand and soak for 1 hour, then seal it and put it into an ultrasonic cleaner, start the program, select the ultrasonic power as 360w, and the ...

Embodiment 2

[0030] This example provides an application test of extracting ceramides from sea red rice bran by using double-enzymatic enzymolysis combined with ultrasonic thermal extraction technology.

[0031] The extraction process in this example is the same as that in Example 1, except that the concentration of the enzymatic hydrolysis solution is 0.4w / v%, the temperature of the enzymatic hydrolysis is 40°C, the pH of the enzymatic hydrolysis is 6, and the enzymatic hydrolysis time is 90 min; 400 mesh silica gel, the ratio of the loading amount of the ceramide extract to the silica gel is 3:100, and the elution speed of petroleum ether and ethyl acetate is 3BV / h; in the SPE small column, the working pressure is 5bar .

Embodiment 3

[0033] This example provides an application test of extracting ceramides from sea red rice bran by using double-enzymatic enzymolysis combined with ultrasonic thermal extraction technology.

[0034] The extraction process in this example is the same as that in Example 1, except that the concentration of the enzymatic hydrolysis solution is 0.5w / v%, the temperature of the enzymatic hydrolysis is 45°C, the pH of the enzymatic hydrolysis is 7, the enzymatic hydrolysis time is 120 min, and the concentration of the ethanol solution is 95 %; the ratio of the loading amount of the ceramide extract and the silica gel is 5:100, and the elution speed of the petroleum ether and ethyl acetate is 4BV / h; the consumption of the ethyl acetate and acetone is 2.5BV, The elution rate was 1.5 BV / h and the working pressure was 20 bar.

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Abstract

The invention discloses a method for extracting ceramide from malus micromalus rice bran, and belongs to the technical field of separation and purification. The method comprises the following steps: performing two-enzyme enzymolysis on rice bran through sphingomyelinase and cellulase, filtering, soaking with an ethanol solution, performing ultrasonic heat extraction, extracting with petroleum ether to obtain a ceramide extract, and performing silica gel column chromatography and SPE (Solid Phase Extraction) small column on the ceramide extract to obtain ceramide. According to the method, a double-enzyme composite enzymolysis method is combined with ultrasonic heat extraction and SPE column chromatography technologies, so that efficient extraction and purification of ceramide in the malus micromalus rice bran are realized.

Description

technical field [0001] The invention belongs to the technical field of enzymolysis, relates to the separation and purification of functional components, and in particular relates to a method for extracting ceramides from sea red rice bran. Background technique [0002] my country is a major grain-producing country with rice as the main grain. The annual output of rice can reach more than 180 million tons, and rice bran accounts for 5% to 8% of rice. It is a high-quality renewable resource. However, due to the in-depth development and utilization of rice bran. Levels are low and the vast majority is processed as feed. Sea rice is a new variety of saline-alkali-tolerant rice that grows on the beach. The by-product of sea red rice processing, sea red rice bran is rich in nutrients, including functional components such as dietary fiber, protein, vitamins, polysaccharides, fatty acids, phytic acid and minerals, and the content of various nutrients is much higher than that of whit...

Claims

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Application Information

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IPC IPC(8): C12P13/02C07C231/24C07C235/08C07C233/18
CPCC12P13/02C07C231/24C07C235/08C07C233/18
Inventor 钟赛意王刚刘寿春陈建平李瑞宋兵兵刘晓菲叶盛权
Owner GUANGDONG OCEAN UNIVERSITY
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